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Reports on the purification of the native trehalose/maltose-binding protein (TMBP), the cloning and sequencing of the malEFG gene cluster, and the expression of the malE gene in Escherichia coli, and the purification and characterization of its encoded binding protein. Results of the study.

Examines the synthesis and characterization of cytoplasmic trehalase of Escherichia coli. Bacterial strains and growth conditions; Cloning of TreF; Isolation of a mutant exhibiting an elevated level of chromosomally encoded TreF; Purification and properties of TreF.

Describes the molecular characterization of the treB gene that encodes the Escherichia coli enzyme II specific for trehalose. Ability of a gene bank of partially Sau3A-digested Escherichia coli DNA ligated in plasmid pBR322 to complement a mutant unable to metabolize trehalose at low osmolarity; Detection of one major transcript; Gene product of treB.

Reports on an improvement of a procedure using Escherichia coli to synthesize 14C-labeled trehalose from [14C]glucose. Preparative synthesis; Method for producing trehalose from glucose; Advantage of using E. coli strain.