1. Abundance of Mobilized Colistin Resistance Gene ( mcr-1 ) in Commensal Escherichia coli from Diverse Sources.
- Author
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Das T, Islam MZ, Rana EA, Dutta A, Ahmed S, Barua H, and Biswas PK
- Subjects
- Animals, Chickens, Drug Resistance, Multiple, Bacterial genetics, Escherichia coli isolation & purification, Escherichia coli Proteins genetics, Farms, Food Microbiology, Genes, Bacterial, Humans, Microbial Sensitivity Tests, Anti-Bacterial Agents pharmacology, Colistin pharmacology, Escherichia coli drug effects, Escherichia coli genetics
- Abstract
Aims: Antimicrobial resistance (AMR) spreads not only by pathogenic but also by commensal bacteria, and the latter can become a reservoir for resistance genes. This study was aimed to investigate the AMR patterns along with the presence of mobilized colistin resistance ( mcr ) genes in commensal Escherichia coli circulating in chickens, farm environments, street foods, and human patients. Materials and Methods: By a cross-sectional survey, isolates obtained from 530 samples were tested for their AMR profiles against 9 antimicrobials. Minimum inhibitory concentration (MIC) of the phenotypically colistin-resistant isolates was determined and screened for a set of mcr genes followed by sequencing of mcr-1 gene in the multidrug-resistant (MDR) isolates. Results: A total of 313 E. coli strains were isolated and confirmed by polymerase chain reaction. Antimicrobial susceptibility testing revealed that about 98% (confidence interval [95% CI] 95-99) of the isolates were MDR, and 58% (95% CI 52-63) isolates exhibited resistance to colistin. MIC values of colistin against the isolates ranged from 4 to 64 mg/L. Except for human patients, 20.4% colistin-resistant isolates from other sources of isolation had mcr-1 gene. Conclusions: There is abundance of commensal MDR E. coli strains with the acquisition of mcr-1 gene circulating in chickens and farm environments in Bangladesh.
- Published
- 2021
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