1. Cell lysis directed by [[sigma].sup.E] in early stationary phase and effect of induction of the rpoE gene on global gene expression in Escherichia coli
- Author
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Kabir, Shahinur, Yamashita, Daisuke, Koyama, Satoshi, Oshima, Taku, Kurokawa, Ken, Maeda, Maki, Tsunedomi, Ryouichi, Murata, Masayuki, Wada, Chieko, Mori, Hirotada, and Yamada, Mamoru
- Subjects
Gene expression -- Research ,DNA microarrays -- Research ,Escherichia coli -- Genetic aspects ,Genetic research ,Biological sciences - Abstract
It has been shown that Escherichia coli cells with increased expression of the rpoE gene encoding [[sigma].sup.E] exhibit enhanced cell lysis in early stationary phase. Further analysis of the lysis phenomenon was performed using a transient expression system of the rpoE gene and by DNA microarray. The former analysis revealed a [[sigma].sup.E]-directed cell lysis, specific for early stationary phase but not for the exponential phase. The microarray analysis with RNAs from exponential and early stationary phase cells revealed that a large number of genes were up-or down-regulated when the rpoE gene was induced, and that several genes were induced in a phase-specific manner. The upregulated genes include many previously identified [[sigma].sup.E] regulon genes, suggesting that a large number of genes are under the control of [[sigma].sup.E] in this organism. These genes are involved in various cellular activities, including the cell envelope, cellular processes, regulatory functions, transport and translation. Genes that are presumably related to phase-specific cell lysis in E. coli are discussed.
- Published
- 2005