1. Knockdown of long non-coding RNA TP73-AS1 inhibits cell proliferation and induces apoptosis in esophageal squamous cell carcinoma.
- Author
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Zang W, Wang T, Wang Y, Chen X, Du Y, Sun Q, Li M, Dong Z, and Zhao G
- Subjects
- Animals, Apoptosis, Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell metabolism, Case-Control Studies, Cell Proliferation, Esophageal Neoplasms genetics, Esophageal Neoplasms metabolism, Female, Follow-Up Studies, Humans, Hydroxybutyrate Dehydrogenase genetics, Hydroxybutyrate Dehydrogenase metabolism, Lymphatic Metastasis, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Middle Aged, Neoplasm Staging, Prognosis, RNA, Long Noncoding genetics, RNA, Long Noncoding metabolism, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, Biomarkers, Tumor genetics, Carcinoma, Squamous Cell secondary, Esophageal Neoplasms pathology, Hydroxybutyrate Dehydrogenase antagonists & inhibitors, RNA, Long Noncoding antagonists & inhibitors
- Abstract
Recent studies have shown that long non-coding RNAs (lncRNAs) are involved in a variety of biological processes and diseases in humans, including cancer. Our study serves as the first comprehensive analysis of lncRNA TP73-AS1 in esophageal cancer. We utilized a lncRNA microarray to analyze the expression profile of lncRNAs in esophageal squamous cell carcinoma. Our results show that lncRNA TP73-AS1 and BDH2 levels are generally upregulated in esophageal cancer tissues and are strongly correlated with tumor location or TNM stage in clinical samples. LncRNA TP73-AS1 knockdown inhibited BDH2 expression in EC9706 and KYSE30 cells, whereas BDH2 knockdown repressed esophageal cancer cell proliferation and induced apoptosis via the caspase-3 dependent apoptotic pathway. Overexpression of BDH2 in lncRNA TP73-AS1 knockdown cells partially rescued cell proliferation rates and suppressed apoptosis. In mouse xenografts, tumor size was reduced in lncRNA TP73-ASI siRNA-transfected tumors, suggesting that downregulation of lncRNA TP73-AS1 attenuated EC proliferation in vitro and in vivo. In addition, BDH2 or lncRNA TP73-AS1 knockdown enhanced the chemosensitivity of esophageal cancer cells to 5-FU and cisplatin. Our results suggest that lncRNA TP73-AS1 may be a novel prognostic biomarker that could serve as a potential therapeutic target for the treatment of esophageal cancer.
- Published
- 2016
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