1. Chemical characterization of Copaiba essential oil and study of its cellular cytotoxicity.
- Author
-
Marques dos Santos, Jacqueline, Barbosa de Souza, Valéria, Almeida Schenka, André, de Barros Mazon, Silvia, Alberici Oliveira, Rosana Maria, Queiroga, Carmen Lucia, Barbosa da Silva Cunha, Ildenize, Bonilha Dezena, Renan Marcel, Nogueira Eberlim, Marcos, and Pires Rosa, Paulo César
- Subjects
- *
ESSENTIAL oils , *CYTOTOXINS , *PEARSON correlation (Statistics) , *TERPENES - Abstract
Introduction: copaifera is a genus of plants that comprises several species that produce copaiba oil, which is widely used for various purposes, such as healing, antiinflammatory, antimicrobial, wound antiseptic, anti-tumor, among other functions. There are very few chemical studies to characterize copaiba oil. Aim: To characterize 3 different species of copaiba oil Subsequently, the effects of oils were estimated on neoplastic cells in a human glioma protocol (U251). Methods: 2 methods of analysis were used for the chemical characterization, GC-MS and ESI-MS. Results: Through these analytical techniques, 20 types of components were found in the oils. Obtaining the cell viability and cytotoxicity of the oil we performed two methods, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Neutral Red (NR), both assays were quantified by spectrophotometry. Concentrations of 1 to 10-5 µg/mL were used for 3 different species of copaiba oil and doxorubicin hydrochloride was used as a positive control. The 3 different species of copaiba oil from Pernambuco (oil 1) and Manaus (oils 2 and 3) were cytotoxic in U251, the IC50 value obtained was 6.171·10-2 μg/mL, 8.344·10-2 μg/mL and 1.385·10-4 μg/mL, respectively. They also presented cytotoxic effect, with IC50, 2.4·10-1 μg/mL, 3.7·10-2 μg/mL and 4.6·10-2 µg/mL, respectively. A high correlation was evidenced between the MTT and Neutral Red studies for the three different species of copaiba oil, with α=0.05 and r values above 0.9, using the Pearson correlation coefficient. All types of resin oils positively affected cell proliferation of in vitro studies with a correspondence between concentration and effect. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF