1. Laccase mediated transformation of 17β-estradiol in soil.
- Author
-
Singh R, Cabrera ML, Radcliffe DE, Zhang H, and Huang Q
- Subjects
- Biodegradation, Environmental, Estradiol analysis, Estradiol chemistry, Humic Substances analysis, Laccase chemistry, Soil classification, Soil Pollutants analysis, Soil Pollutants chemistry, Estradiol metabolism, Laccase metabolism, Soil Pollutants metabolism
- Abstract
It is known that 17β-estradiol (E2) can be transformed by reactions mediated by some oxidoreductases such as laccase in water. Whether or how such reactions can happen in soil is however unknown although they may significantly impact the environmental fate of E2 that is introduced to soil by land application of animal wastes. We herein studied the reaction of E2 in a model soil mediated by laccase, and found that the reaction behaviors differ significantly from those in water partly because of the dramatic difference in laccase stability. We also examined E2 transformation in soil using (14)C-labeling in combination with soil organic matter extraction and size exclusion chromatography, which indicated that applied (14)C radioactivity was preferably bound to humic acids. The study provides useful information for understanding the environmental fate of E2 and for developing a novel soil remediation strategy via enzyme-enhanced humification reactions., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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