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The hypothesis whether estrone (E 1 ) could exhibit a direct action at uterus and white adipose tissue (WAT), under obesity was tested. In uterine tissue of obese rats, E 1 increased nitric oxide (NO) synthesis, and reduced reactive oxygen species (ROS) production. The anti-oxidative action of E 1 was sustained under inflammatory stress or high glucose levels. ICI 182780 or G15 compounds were employed as ER or GPER antagonists respectively. The action of E 1 on ROS release involved ER participation; instead GPER mediated the acute stimulation on NO production. The antioxidative effect depends on NO-ROS balance. NO synthase (NOS) blockage suppressed the reduction in ROS synthesis elicited by E 1 , effect mediated by cNOS and not by iNOS. On WAT explants, E 1 reduced ROS and thiobarbituric acid reactive substances production, and diminished leptin release. In summary, the data provide evidence that, in uterus and WAT, E 1 counteracts inflammatory and oxidative stress induced by obesity., Competing Interests: Declaration of competing interest The author(s) declare(s) that there is no conflict of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)