1. Proteomic analysis of proteins increased or reduced by ethanol of Lactobacillus plantarum ST4 isolated from Makgeolli, traditional Korean rice wine.
- Author
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Lee SG, Lee KW, Park TH, Park JY, Han NS, and Kim JH
- Subjects
- Bacterial Proteins analysis, Bacterial Proteins genetics, Fermentation, Gene Expression Regulation, Bacterial, Lactobacillus plantarum chemistry, Lactobacillus plantarum genetics, Molecular Sequence Data, Oryza metabolism, Wine analysis, Bacterial Proteins metabolism, Ethanol metabolism, Lactobacillus plantarum isolation & purification, Lactobacillus plantarum metabolism, Oryza microbiology, Proteomics, Wine microbiology
- Abstract
LAB were isolated from makgeolli locally produced around Jinju, Gyeongnam, S. Korea during spring of 2011. Randomly selected 11 isolates from MRS agar plates were identified first by API CHL 50 kits and then 16S rRNA gene sequencing. All 11 isolates were identified as Lactobacillus plantarum. Among them, ST4 grew in MRS broth with ethanol up to 10%, showing the highest alcohol resistance. L. plantarum ST4 was moderately resistant against acid and bile salts. When cellular proteins of L. plantarum ST4 under ethanol stress were analyzed by two-dimensional gel electrophoresis (2DE), the intensities of 6 spots increased, whereas 22 spots decreased at least 2-fold. Those 28 spots were identified by peptide mass fingerprinting (PMF). FusA2 (elongation factor G) increased 18.8-fold (6% ethanol) compared with control. Other proteins were AtpD (ATP synthase subunit beta), DnaK, GroEL, Tuf (elongation factor Tu), and Npr2 (NADH peroxidase), respectively. Among the 22 proteins decreased in intensities, lactate dehydrogenases (LdhD and LdhL1) were included.
- Published
- 2012
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