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[Objective] Pseudo response regulators (PRRs) are important genes that regulate the flowering pathway in higher plants. The aim of this study is to clone the NtPRR37 gene from Nicotiana tabacum L. and analyze its response to different photoperiods and its effect on flowering, providing a target gene for tobacco flowering regulation. [Method] We cloned NtPRR37 gene from N. tabacum L. by homologous cloning method. Then we performed bioinformatics analysis, tissue-specific expression analysis, and expression pattern analysis of NtPRR37 gene under different light duration treatments. Concurrently, we used Virus Induced Gene Silence (VIGS) to reduce the expression of NtPRR37 and observed the phenotype and detected the expression changes of flowering-related genes. [Result] The fulllength CDS of NtPRR37 was 2 472 bp, encoded a peptide of 823 amino acids with a relative molecular weight of 90.16 kD. It contained REC and CCT domains, which were typical conserved domains of the PRRs gene family. Through homology evolution analysis, it was found that tobacco NtPRR37 belonged to the same branch as PRR37 from Nicotiana tomentosiformis, Nicotiana sylvestris, and Nicotiana benthamiana in evolution. The quantitative reverse transcription-PCR (RT-qPCR) analysis showed that, the expressions of NtPRR37 varied in various tissues of N. tabacum L. at full-bloom stage, with the highest expression in pistils and the lowest expression in lateral roots. Under different photoperiod treatments, the expression of NtPRR37 increased with the prolongation of light exposure. In different light duration treatments, the expression of NtPRR37 increased with the extension of light time, with the lowest expression under darkness, and demonstratted circadian rhythmicity. In NtPRR37-silenced plants, the expression of NtPRR37 was significantly downregulated, and the flowering period was advanced, which may be related to the significant upregulation of flowering-related genes (NtFT4, NtAP1, NtCO, NtSOC1). [Conclusion] The expression of NtPRR37 is regulated by the photoperiod, and NtPRR37 acts as a flowering repressor during the flowering process of tobacco. [ABSTRACT FROM AUTHOR]

In recent years, the medicinal and feeding value of Gelsemium elegans has become increasingly prominent. However, G. elegans is not tolerant to low temperatures during its growth process. Exploring its low temperature response genes may lay the foundation for the research of cold resistance breeding of G. elegans. Lipoxygenase（LOX）has a significant impact on the physiological and biochemical processes of plants in seed aging, stress resistance, and other aspects. The LOX genes of G. elegans in response to low temperature stress were mined from the transcriptome database of G. elegans constructed by our team. A full-length cDNA sequence of the GeLOX1 gene was cloned from the leaves of G. elegans using RT-PCR technology, and its bioinformatics, subcellular localization, gene expression, prokaryotic expression, and plate stress were analyzed. The results showed that the amino acid length of the protein encoded by the GeLOX1 was 761 aa, and the relative molecular weight of the protein was 87.00 kD. It was predicted to be an unstable hydrophilic protein, containing 28 serine phosphorylation sites, 22 threonine phosphorylation sites, and 9 tyrosine phosphorylation sites. The analysis results of evolutionary tree indicated that GeLOX1 belonged to the 9-LOX family. The subcellular localization test results showed that the GeLOX1 was located in the cytoplasm. Real time fluorescence quantitative PCR analysis revealed that the GeLOX1 was highly expressed in the roots of the G. elegans, and its expressions showed a downward trend under 4℃ low temperature stress. After prokaryotic expression induction, the recombinant protein of the GeLOX1 showed a target band at approximately 111 kD, and the accumulation of the recombinant protein reached its peak at 8 h of induction. In addition, the plate stress experiment showed that the prokaryotic expression strain of the GeLOX1 was more sensitive to low temperature stress compared to the control group. The research results indicate that GeLOX1 of G. elegans can respond to low temperature stress. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Hydrobiology / Shuisheng Shengwu Xuebao is the property of Editorial Department of Journal of Hydrobiology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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[Objectives]The study aimed to investigate the sequence and expression characteristics of caffeoyl-CoA O-methyltransferase gene in Oenanthe javanica(OjCCoAOMT) and to understand its role in lignin metabolism. [Methods]The OjCCoAOMT gene was cloned from O.javanica using RT-PCR technique. The bioinformatics methods were used to analyze putative amino acid sequence and RT-qPCR method was used to study the expression profiles of OjCCoAOMT gene in different tissues of O.javanica and under different storage conditions. [Results]Sequence analysis result showed that OjCCoAOMT gene contained an open reading frame(ORF) with length of 726 bp,encoded 241 amino acids. The relative molecular mass of OjCCoAOMT protein was 27.111×103. Theoretical isoelectric point was 5.38. The protein belonged to hydrophobic protein and the ratio of its unordered sequence was 12.45%. Tertiary structure of OjCCoAOMT protein mainly consisted of eight α-helix and seven β-sheet. The analysis results of multiple alignment and phylogenetic tree showed that OjCCoAOMT had the nearest phylogeny with CCoAOMT protein from the same family species Petroselinum crispum. RT-qPCR analysis showed that the expression profiles of OjCCoAOMT gene were significantly different in different tissues(leaf blade and petiole) of ‘Baguazhoushuiqin’ and ‘Nanxuanbaguazhouzishuiqin’. The expression levels of OjCCoAOMT gene were different under various storage conditions,such as room temperature(25℃),low temperature(4℃) and darkness at room temperature(25℃). The expression level was peak at 4℃. Moreover,significant differences also existed between two varieties. [Conclusions]OjCCoAOMT protein has high conservation. The relative expression level of OjCCoAOMT gene has tissue and material specialties under different treatments. Moreover,OjCCoAOMT gene has high expression level under the treatment of 4℃. [ABSTRACT FROM AUTHOR]

AP2/ERF is a kind of important transcription factors widely existing in plants. AP2/ERF regulate the expression of some genes involved in abiotic stress, and help plants improve the ability of adversity stress. We obtained a gene LaAP2 by cloning a significantly up-regulated expression AP2/ERF family gene sequence by RT-PCR technology, based on the previous analysis of Lepidium apetalum transcriptome database. Bioinformatics analysis of LaAP2 encoded amino acid sequence composition, physical and chemical properties, structure, hydrophobicity, transmembrane region, signal peptide, subcellular localization and secondary protein structure. Real-time PCR was used to detect the expression of LaAP2 gene at 4 ℃ low temperature stress. The result showed that the full-length cDNA of the gene was 1 005 bp, and the encoded amino acid sequence contains a AP2 and a B3 domain, belonging to the AP2/ERF transcription factor RAV subfamily. The mole-cular weight of putative LaAP2 protein was 37.744 67 kD and the isoelectric point was 9.49. The amino acid sequence has high homology with Camelina sativa, Arabidopsis thaliana and rape. Phylogenetic analysis showed that the genetic relationship was close to that of A. thaliana. The amino acid sequence analysis showed that LaAP2 gene encoding protein had no signal peptide segments, no transmembrane region, did not belong to the secreted protein. It could be the hydrophilic protein. The possibility of localization in cytoplasm was 56.5%, and in nucleus was 21.7%. The main structural elements were random coil, extending strand and alpha helix. We analyzed the expression of AP2/ERF gene in Lepidium apetalum seedlings under 4 ℃ low temperature stress through Real-time PCR technology, which showed that LaAP2 gene expression was decreased firstly and then increased. The results suggest that LaAP2 play a role in regulating the resistance of low temperature stress in Lepidieae seedling. This study lays the foundation for further study on the function of LaAP2 on the L. apetalum seed germination and seedling growth in tolerance to low temperature. [ABSTRACT FROM AUTHOR]

Phytochrome(PHY)is one of the most important light receptors in plants. It is involved in many physiological processes such as the regulation of seed germination, seedling growth, stem elongation, cotyledon extension, and flowering control. In this study, a cDNA encoding PHYA was firstly amplified from Akebia trifoliata by using the RT-PCR technique(designated as AktrPHYA1, GenBank accession number: KP864055), which consists of 3 496 bp, with a 3 426-bp open reading frame(ORF)encoding a 1 141-amino-acid protein. The N-terminal of AktrPHYA1 is dedicates for light acceptation and contains a GAF domain and a phytochrome domain. The C-terminal is a light-regulated region, and is comprised of two PAS domains, a His Kinase A domain and a Histidine kinase-like ATPase domain. The homology protein alignment showed that the AktrPHYA1 shared 83% and 82% identities with the PHYAs from Nelumbo nucifera and Aquileqia formosa, respectively. Phylogenetic tree analysis indicated that monocots and dicots gathered into two branches. In the dicots, Akebia trifoliate, Nelumbo nucifera and Aquileqia formosa clustered together, indicating they are closely genetically related. Expression analysis indicated that AktrPHYA1 was detected in all tissues under investigation, including leaves, stems, male and female flowers and seeds. It was the most strongly expressed in seeds, whereas the most weakly in leaves. The expression pattern implied the likely roles of AktrPHYA1 in Akebia trifoliata. This work will provide information for further research of its functions in plants. [ABSTRACT FROM AUTHOR]