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V-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors play important roles in the processes of plant growth, development, and stress responses. In this study, a full-length cDNA sequence of a MYB gene, designated FvMYB1, was isolated from Arizona ash (Fraxinus velutina Torr.) for the first time. Sequence analysis showed that the deduced amino acid sequence of FvMYB1 encoded novel MYB proteins with single DNA binding domains. Based on the R3 domain amino acid sequence, the FvMYB1 was closely related to some proteins whose functions were known. The expression pattern of FvMYB1 in different organs of Arizona ash was analyzed by semiquantitative RT-PCR and real-time PCR (qRT-PCR). Results showed that this gene was widely distributed in all the tested organs and the expression level of FvMYB1 was the highest in stems and the lowest in roots. The gene expression level decreased dramatically in roots under salt treatment (300 mM, 24 h), while no obvious change was observed in stems. Subcellular localization indicated that FvMYB1 was localized in the nucleus. This study will lead to further research in resistance to abiotic stress in Arizona ash. [ABSTRACT FROM AUTHOR]

Five putative acyl carrier protein (ACP) cDNAs were isolated from developing peanut seeds by searching ESTs of a peanut immature-seed cDNA library and PCR-based cloning. Five peanut ACPs contained a strictly conserved Ser residue in the Asp-Ser-Leu (DSL) motif, which is an important characteristic of ACPs in plants and bacteria. Three AhACPs, AhACP1, AhACP4, AhACP5, were predicted to be located in chloroplast, while two AhACPs, AhACP2 and AhACP3, in mitochondria. Comparison of genomic DNA and cDNA sequences demonstrated three chloroplast ACPs (cpACPs) comprising of four exons and three introns while two mitochondrial ACPs (mtACPs) contained two exons and one intron. More than two homologs of each AhACP were expressed in developing peanut seeds. Most homologs were verified by corresponding genomic DNA sequences. Semi-quantitative RT-PCR analysis suggested AhACP1 was a seed-predominant ACP isoform. AhACP4 and AhACP5 showed same mRNA profile in different organs and during seed development. Two mtACPs expressed highly in peanut flower tissue which was distinct from three cpACPs. [ABSTRACT FROM AUTHOR]