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Start Over Author "Rawan Sayed" Topic extracellular vesicles
4 results on '"Rawan Sayed"'

1. Extracellular Vesicles of Alzheimer's Disease Patients as a Biomarker for Disease Progression.

Author

Aharon A, Spector P, Ahmad RS, Horrany N, Sabbach A, Brenner B, and Aharon-Peretz J

Subjects
Aged, Alzheimer Disease genetics, Cytokines metabolism, Exosomes metabolism, Female, Gene Expression Regulation, Humans, Intercellular Signaling Peptides and Proteins metabolism, Male, MicroRNAs genetics, MicroRNAs metabolism, Middle Aged, Myelin-Oligodendrocyte Glycoprotein metabolism, Neurons metabolism, Severity of Illness Index, Alzheimer Disease metabolism, Alzheimer Disease pathology, Biomarkers metabolism, Disease Progression, Extracellular Vesicles metabolism
Abstract

Alzheimer's disease (AD) is a progressive neurodegenerative brain pathology and the most common form of dementia. Evidence suggests that extracellular vesicles (EVs) containing cytokines and microRNA are involved in inflammation regulation. The current study aimed to explore a potential impact of AD patients' EVs on disease progression. Blood samples were collected after obtaining signed informed consent (No. 0462-14-RMB) from 42 AD patients at three stages of disease severity and from 19 healthy controls (HC). EV size and concentration were studied by nanotracking analysis. EV membrane antigens were defined by flow cytometry and Western blot; EV protein contents were screened by protein array; the miRNA content was screened by nanostring technology and validated by RT-PCR. HC and AD patients' EVs consisted of a mixture of small (< 100 nm) and larger vesicles. The myelin oligodendrocyte glycoprotein (MOG) expression on EVs correlated with disease severity. EVs of patients with moderate and severe AD had significantly higher levels of MOG, compared with mild AD patients. Levels of EVs expressing the axonal glycoprotein CD171 were significantly higher in severe AD patients than in HC. Increase in endothelial EVs was observed in AD patients. An above twofold increase was found in the content of inflammatory cytokines and > 50% decrease in growth factors in AD patients' EVs compared with HC-EVs. Levels of let-7g-5p, miR126-3p, miR142-3p, miR-146a-5p, and mir223-3p correlated with disease severity. Neural damage, specific miRNA downregulation, and inflammatory cytokine upregulation, found in patients' EVs, might be used as a biomarker reflecting AD severity.

Published
2020
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2. Extracellular Vesicles of Alzheimer's Disease Patients as a Biomarker for Disease Progression

Author

Rawan Sayed Ahmad, Judith Aharon-Peretz, Annie Sabbach, Polina Spector, Anat Aharon, Nizar Horrany, and Benjamin Brenner

Subjects
0301 basic medicine, Male, medicine.medical_treatment, Neuroscience (miscellaneous), Inflammation, Disease, Exosomes, Severity of Illness Index, Flow cytometry, 03 medical and health sciences, Cellular and Molecular Neuroscience, Extracellular Vesicles, 0302 clinical medicine, Downregulation and upregulation, Western blot, Alzheimer Disease, Medicine, Dementia, Humans, Aged, Neurons, medicine.diagnostic_test, business.industry, Middle Aged, medicine.disease, MicroRNAs, 030104 developmental biology, Cytokine, Neurology, Gene Expression Regulation, Immunology, Disease Progression, Biomarker (medicine), Cytokines, Intercellular Signaling Peptides and Proteins, Female, Myelin-Oligodendrocyte Glycoprotein, medicine.symptom, business, 030217 neurology & neurosurgery, Biomarkers
Abstract

Alzheimer's disease (AD) is a progressive neurodegenerative brain pathology and the most common form of dementia. Evidence suggests that extracellular vesicles (EVs) containing cytokines and microRNA are involved in inflammation regulation. The current study aimed to explore a potential impact of AD patients' EVs on disease progression. Blood samples were collected after obtaining signed informed consent (No. 0462-14-RMB) from 42 AD patients at three stages of disease severity and from 19 healthy controls (HC). EV size and concentration were studied by nanotracking analysis. EV membrane antigens were defined by flow cytometry and Western blot; EV protein contents were screened by protein array; the miRNA content was screened by nanostring technology and validated by RT-PCR. HC and AD patients' EVs consisted of a mixture of small ( 100 nm) and larger vesicles. The myelin oligodendrocyte glycoprotein (MOG) expression on EVs correlated with disease severity. EVs of patients with moderate and severe AD had significantly higher levels of MOG, compared with mild AD patients. Levels of EVs expressing the axonal glycoprotein CD171 were significantly higher in severe AD patients than in HC. Increase in endothelial EVs was observed in AD patients. An above twofold increase was found in the content of inflammatory cytokines and 50% decrease in growth factors in AD patients' EVs compared with HC-EVs. Levels of let-7g-5p, miR126-3p, miR142-3p, miR-146a-5p, and mir223-3p correlated with disease severity. Neural damage, specific miRNA downregulation, and inflammatory cytokine upregulation, found in patients' EVs, might be used as a biomarker reflecting AD severity.

Published
2020

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