1. Protective effect of royal jelly on fluoride-induced nephrotoxicity in rats via the some protein biomarkers signalling pathways: a new approach for kidney damage.
- Author
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Aslan A, Beyaz S, Gok O, Can MI, Parlak G, Gundogdu R, Ozercan IH, and Baspinar S
- Subjects
- Animals, Male, Rats, Antioxidants metabolism, bcl-2-Associated X Protein metabolism, Biomarkers, Brain-Derived Neurotrophic Factor metabolism, Brain-Derived Neurotrophic Factor pharmacology, Caspase 3 metabolism, Caspase 6 metabolism, Caspase 6 pharmacology, Caspase 9 metabolism, Cyclooxygenase 2 metabolism, Cyclooxygenase 2 pharmacology, Fluorides toxicity, Glycogen Synthase Kinase 3 metabolism, Glycogen Synthase Kinase 3 pharmacology, Kidney, Proto-Oncogene Proteins c-bcl-2 metabolism, Proto-Oncogene Proteins c-bcl-2 pharmacology, Rats, Wistar, Tumor Necrosis Factor-alpha metabolism, Vascular Endothelial Growth Factor A metabolism, Kidney Diseases chemically induced, Kidney Diseases drug therapy, Kidney Diseases prevention & control, Fatty Acids pharmacology
- Abstract
Introduction: Protective effect of royal jelly (RJ) on fluoride-induced nephrotoxicity was investigated in this study., Methods: 42 healthy male Wistar rats (n = 42, 8 weeks of age) were divided equally into 6 groups with 7 rats in each; (1) Group-1: Controls fed with standard diet; (2) Group-2: RJ [100 mg/kg] bw (body weight), by oral gavage; (3) Group-3: Fluoride [50 mg/kg] bw, in drinking water; (4) Group-4: Fluoride [100 mg/kg] bw, in drinking water; (5) Group-5: RJ [100 mg/kg] bw, by oral gavage + Fluoride [50 mg/kg] bw, in drinking water; (6) Group-6: RJ [100 mg/kg] bw, by oral gavage + Fluoride [100 mg/kg] bw, in drinking water. After 8 weeks, all rats were decapitated and their kidney tissues were removed for further analysis. The protein expression levels of caspase-3, caspase-6, caspase-9, Bcl-2, Bax, VEGF, GSK-3, BDNF, COX-2 and TNF-α proteins in kidney tissue were analysed by western blotting technique., Results: RJ increased Bcl-2, COX-2, GSK-3, TNF-α and VEGF protein levels and a decreased caspase-3, caspase -6, caspase-9, Bax and BDNF protein levels in fluoride-treated rats., Conclusion: RJ application may have a promising therapeutical potential in the treatment of many diseases in the future by reducing kidney damage.
- Published
- 2022
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