15 results on '"Harwood, Valerie J."'
Search Results
2. The Effect of Protozoa Indigenous to Lakewater and Wastewater on Decay of Fecal Indicator Bacteria and Coliphage
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Korajkic, Asja, McMinn, Brian R., and Harwood, Valerie J.
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Microbiology (medical) ,Infectious Diseases ,bacteriophage ,General Immunology and Microbiology ,fecal indicator bacteria ,Immunology and Allergy ,predation ,ambient water ,wastewater ,Molecular Biology - Abstract
Fecal indicator bacteria (FIB: Escherichia coli and enterococci) are used to assess recreational water quality. Viral indicators (i.e., somatic and F+ coliphage), could improve the prediction of viral pathogens in recreational waters, however, the impact of environmental factors, including the effect of predatory protozoa source, on their survival in water is poorly understood. We investigated the effect of lakewater or wastewater protozoa, on the decay (decreasing concentrations over time) of culturable FIB and coliphages under sunlight and shaded conditions. FIB decay was generally greater than the coliphages and was more rapid when indicators were exposed to lake vs. wastewater protozoa. F+ coliphage decay was the least affected by experimental variables. Somatic coliphage decayed fastest in the presence of wastewater protozoa and sunlight, though their decay under shaded conditions was-10-fold less than F+ after 14 days. The protozoa source consistently contributed significantly to the decay of FIB, and somatic, though not the F+ coliphage. Sunlight generally accelerated decay, and shade reduced somatic coliphage decay to the lowest level among all the indicators. Differential responses of FIB, somatic, and F+ coliphages to environmental factors support the need for studies that address the relationship between the decay of coliphages and viral pathogens under environmentally relevant conditions.
- Published
- 2023
3. Microbes in beach sands: integrating environment, ecology and public health
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Whitman, Richard L., Harwood, Valerie J., Edge, Thomas A., Nevers, Meredith B., Byappanahalli, Muruleedhara, Vijayavel, Kannappan, Brandão, João, Sadowsky, Michael J., Alm, Elizabeth Wheeler, Crowe, Allan, Ferguson, Donna, Ge, Zhongfu, Halliday, Elizabeth, Kinzelman, Julie, Kleinheinz, Greg, Przybyla-Kelly, Kasia, Staley, Christopher, Staley, Zachery, and Solo-Gabriele, Helena M.
- Published
- 2014
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4. Current Status of Marker Genes of Bacteroides and Related Taxa for Identifying Sewage Pollution in Environmental Waters.
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Ahmed, Warish, Hughes, Bridie, and Harwood, Valerie J.
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BACTEROIDES ,SEWAGE ,POLYMERASE chain reaction ,WATER pollution ,DNA - Abstract
Microbial source tracking (MST) endeavors to determine sources of fecal pollution in environmental waters by capitalizing on the association of certain microorganisms with the gastrointestinal tract and feces of specific animal groups. Several decades of research have shown that bacteria belonging to the gut-associated order Bacteroidales, and particularly the genus Bacteroides, tend to co-evolve with the host, and are, therefore, particularly suitable candidates for MST applications. This review summarizes the current research on MST methods that employ genes belonging to Bacteroidales/Bacteroides as tracers or "markers" of sewage pollution, including known advantages and deficiencies of the many polymerase chain reaction (PCR)-based methods that have been published since 2000. Host specificity is a paramount criterion for confidence that detection of a marker is a true indicator of the target host. Host sensitivity, or the prevalence of the marker in feces/waste from the target host, is necessary for confidence that absence of the marker is indicative of the absence of the pollution source. Each of these parameters can vary widely depending on the type of waste assessed and the geographic location. Differential decay characteristics of bacterial targets and their associated DNA contribute to challenges in interpreting MST results in the context of human health risks. The HF183 marker, derived from the 16S rRNA gene of Bacteroides dorei and closely related taxa, has been used for almost two decades in MST studies, and is well characterized regarding host sensitivity and specificity, and in prevalence and concentration in sewage in many countries. Other markers such as HumM
2 and HumM3 show promise, but require further performance testing to demonstrate their widespread utility. An important limitation of the one-marker-one-assay approach commonly used for MST is that given the complexities of microbial persistence in environmental waters, and the methodological challenges of quantitative PCR (qPCR) in such samples, the absence of a given marker does not ensure the absence of fecal pollution in the source water. Approaches under development, such as microarray and community analysis, have the potential to improve MST practices, thereby increasing our ability to protect human and ecosystem health. [ABSTRACT FROM AUTHOR]- Published
- 2016
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5. Agrochemicals indirectly increase survival of E. coli O157:H7 and indicator bacteria by reducing ecosystem services.
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Staley, Zachery R., Rohr, Jason R., Senkbeil, Jacob K., and Harwood, Valerie J.
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RUNOFF ,AGRICULTURAL pollution ,AGRICULTURAL chemicals ,ZOONOSES ,AQUATIC ecology ,ATRAZINE & the environment ,CHLOROTHALONIL - Abstract
Storm water and agricultural runoff frequently contain agrochemicals, fecal indicator bacteria (FIB), and zoonotic pathogens. Entry of such contaminants into aquatic ecosystems may affect ecology and human health. This study tested the hypothesis that the herbicide atrazine and the fungicide chlorothalonil indirectly affect the survival of FIB (Escherichia coli and Enterococcus faecalis) and a pathogen (E. coli O157:H7) by altering densities of protozoan predators or by altering competition from autochthonous bacteria. Streptomycin-resistant E. coli, En. faecalis, and E. coli O157:H7 were added to microcosms composed of Florida river water containing natural protozoan and bacterial populations. FIB, pathogen, and protozoan densities were monitored over six days. Known metabolic inhibitors, cycloheximide and streptomycin, were used to inhibit autochthonous protozoa or bacteria, respectively. The inhibitors made it possible to isolate the effects of predation or competition on survival of allochthonous bacteria, and each treatment increased the survival of FIB and pathogens. Chlorothalonil's effect was similar to that of cycloheximide, significantly reducing protozoan densities and elevating densities of FIB and pathogens relative to the control. Atrazine treatment did not affect protozoan densities, but, through an effect on competition, resulted in significantly greater densities of En. faecalis and E. coli O157:H7. Hence, by reducing predaceous protozoa and bacterial competitors that facilitate purifying water bodies of FIBs and human pathogens, chlorothalonil and atrazine indirectly diminished an ecosystem service of fresh water. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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6. Microbial source tracking markers for detection of fecal contamination in environmental waters: relationships between pathogens and human health outcomes.
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Harwood, Valerie J., Staley, Christopher, Badgley, Brian D., Borges, Kim, and Korajkic, Asja
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FECAL contamination , *AQUATIC microbiology , *PATHOGENIC microorganisms , *ENVIRONMENTAL health , *NUCLEOTIDE sequence , *ENVIRONMENTAL research , *MICROBIAL ecology - Abstract
Microbial source tracking ( MST) describes a suite of methods and an investigative strategy for determination of fecal pollution sources in environmental waters that rely on the association of certain fecal microorganisms with a particular host. MST is used to assess recreational water quality and associated human health risk, and total maximum daily load allocations. Many methods rely on signature molecules (markers) such as DNA sequences of host-associated microorganisms. Human sewage pollution is among the greatest concerns for human health due to (1) the known risk of exposure to human waste and (2) the public and regulatory will to reduce sewage pollution; however, methods to identify animal sources are receiving increasing attention as our understanding of zoonotic disease potential improves. Here, we review the performance of MST methods in initial reports and field studies, with particular emphasis on quantitative PCR ( qPCR). Relationships among human-associated MST markers, fecal indicator bacteria, pathogens, and human health outcomes are presented along with recommendations for future research. An integrated understanding of the advantages and drawbacks of the many MST methods targeting human sources advanced over the past several decades will benefit managers, regulators, researchers, and other users of this rapidly growing area of environmental microbiology. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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7. Environmental Persistence and Naturalization of Fecal Indicator Organisms
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Ferguson, Donna, Signoretto, Caterina, Hagedorn, Charles, editor, Blanch, Anicet R., editor, and Harwood, Valerie J., editor
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- 2011
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8. Relating MST Results to Fecal Indicator Bacteria, Pathogens, and Standards
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Kinzelman, Julie, Kay, David, Pond, Kathy, Hagedorn, Charles, editor, Blanch, Anicet R., editor, and Harwood, Valerie J., editor
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- 2011
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9. Precipitation influences pathogenic bacteria and antibiotic resistance gene abundance in storm drain outfalls in coastal sub-tropical waters.
- Author
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Ahmed, Warish, Zhang, Qian, Lobos, Aldo, Senkbeil, Jacob, Sadowsky, Michael J., Harwood, Valerie J., Saeidi, Nazanin, Marinoni, Oswald, and Ishii, Satoshi
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STORM drains , *PATHOGENIC bacteria , *GENOTYPE-environment interaction , *METEOROLOGICAL precipitation , *HEALTH risk assessment , *TERRITORIAL waters - Abstract
Stormwater contamination can threaten the health of aquatic ecosystems and human exposed to runoff via nutrient and pathogen influxes. In this study, the concentrations of 11 bacterial pathogens and 47 antibiotic resistance genes (ARGs) were determined by using high-throughput microfluidic qPCR (MFQPCR) in several storm drain outfalls (SDOs) during dry and wet weather in Tampa Bay, Florida, USA. Data generated in this study were also compared with the levels of fecal indicator bacteria (FIB) and sewage-associated molecular markers (i.e., Bacteroides HF183 and crAssphage markers) in same SDOs collected in a recent study ( Ahmed et al., 2018 ). Concentration of FIB, sewage-associated markers, bacterial pathogens and many ARGs in water samples were relatively high and SDOs may be potentially hotspots for microbial contamination in Tampa Bay. Mean concentrations of culturable E. coli and Enterococcus spp. were tenfold higher in wet compared to dry weather. The majority of microbiological contaminants followed this trend. E. coli eaeA , encoding the virulence factor intimin, was correlated with levels of 20 ARGs, and was more frequently detected in wet weather than dry weather samples. The bla KPC gene associated with carbapenem resistant Enterobacteriaceae and the beta-lactam resistant gene ( bla NPS ) were only detected in wet weather samples. Frequency of integron genes Intl2 and Intl3 detection increased by 42% in wet weather samples. Culturable E. coli and Enterococcus spp. significantly correlated with 19 of 47 (40%) ARG tested. Sewage-associated markers crAssphage and HF183 significantly correlated ( p < 0.05) with the following ARGs: intl1 , sul1 , tet (M), ampC , mexB , and tet (W). The presence of sewage-associated marker genes along with ARGs associated with sewage suggested that aging sewage infrastructure contributed to contaminant loading in the Bay. Further research should focus on collecting spatial and temporal data on the microbiological contaminants especially viruses in SDOs. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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10. Evaluation of the novel crAssphage marker for sewage pollution tracking in storm drain outfalls in Tampa, Florida.
- Author
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Ahmed, Warish, Lobos, Aldo, Senkbeil, Jacob, Peraud, Jayme, Gallard, Javier, and Harwood, Valerie J.
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STORM drains , *SEWAGE purification , *BACTERIOPHAGES , *FECAL contamination , *NUCLEIC acid isolation methods - Abstract
CrAssphage are recently-discovered DNA bacteriophages that are prevalent and abundant in human feces and sewage. We assessed the performance characteristics of a crAssphage quantitative PCR (qPCR) assay for quantifying sewage impacts in stormwater and surface water in subtropical Tampa, Florida. The mean concentrations of crAssphage in untreated sewage ranged from 9.08 to 9.98 log 10 gene copies/L. Specificity was 0.927 against 83 non-human fecal reference samples and the sensitivity was 1.0. Cross-reactivity was observed in DNA extracted from soiled poultry litter but the concentrations were substantially lower than untreated sewage. The presence of the crAssphage marker was monitored in water samples from storm drain outfalls during dry and wet weather conditions in Tampa, Florida. In dry weather conditions, 41.6% of storm drain outfalls samples were positive for the crAssphage marker and the concentrations ranged from 3.60 to 4.65 log 10 gene copies/L of water. After a significant rain event, 66.6% of stormwater outlet samples were positive for the crAssphage marker and the concentration ranged from 3.62 to 4.91 log 10 gene copies/L of water. The presence of the most commonly used Bacteroides HF183 marker in storm drain outfalls was also tested along with the crAssphage. Thirteen samples (55%) were either positive (i.e., both markers were present) or negative (i.e., both markers were absent) for both the markers. Due to the observed cross-reactivity of this marker with DNA extracted from poultry litter samples, it is recommended that this marker should be used in conjunction with additional markers such as HF183. Our data indicate that the crAssphage marker is highly sensitive to sewage, is adequately specific, and will be a valuable addition to the MST toolbox. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
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11. Transport and attenuation of Salmonella enterica, fecal indicator bacteria and a poultry litter marker gene are correlated in soil columns.
- Author
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Mantha, Sirisha, Anderson, Angela, Acharya, Saraswati Poudel, Harwood, Valerie J., and Weidhaas, Jennifer
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POULTRY , *AGRICULTURE , *IRRIGATION , *PATHOGENIC microorganisms , *GROUNDWATER - Abstract
Millions of tons of fecal-contaminated poultry litter are applied to U.S. agricultural fields annually. Precipitation and irrigation facilitate transport of fecal-derived pathogens and fecal indicator bacteria (FIB) to groundwater. The goal of this study was to compare transport of pathogens, FIB, and a microbial source tracking marker gene for poultry litter (LA35) in a simulated soil-to-groundwater system. Nine laboratory soil columns containing four different soil types were used to evaluate microbial transport to groundwater via infiltration. Quantitative polymerase chain reaction was used to monitor Salmonella enterica Typhimurium, Escherichia coli , Enterococcus spp., Brevibacterium sp. LA35 and Bacteroidales leached from soil columns inoculated with poultry litter. S . enterica was correlated with LA35 poultry litter marker gene and FIB concentrations in column soils containing organic matter, but not in acid washed sands. In contrast, S . enterica was found to correlate with LA35 and FIB in the leachate from columns containing sand, but not with leachate from organic soil columns. The majority of recovered DNA was found in leachate of predominately sandy soil columns, and in the soil of loamy columns. At least 90% of the DNA retained in soils for each microbial target was found in the top 3 cm of the column. These studies suggest that poultry litter associated pathogens and FIB are rapidly released from litter, but are influenced by complex attenuation mechanisms during infiltration, including soil type. This study advances our understanding of the potential for subsurface transport of poultry litter associated pathogens and FIB, and support the use of the LA35 marker gene for evaluating poultry litter impacts on groundwater. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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12. Multiple lines of evidence to identify sewage as the cause of water quality impairment in an urbanized tropical watershed.
- Author
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Kirs, Marek, Kisand, Veljo, Wong, Mayee, Caffaro-Filho, Roberto A., Moravcik, Philip, Harwood, Valerie J., Yoneyama, Bunnie, and Fujioka, Roger S.
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SEWAGE , *WATER quality , *WATERSHEDS , *ESCHERICHIA coli ,HEALTH risk factors - Abstract
Indicator bacteria, which are conventionally used to evaluate recreational water quality, can originate from various non-human enteric and extra-enteric sources, hence they may not be indicative of human health risk nor do they provide information on the sources of contamination. In this study we utilized traditional (enterococci and Escherichia coli ) and alternative ( Clostridium perfringens) indicator bacteria, F + -specific coliphage, molecular markers for microorganisms associated with human sewage (human-associated Bacteroides and polyomaviruses), and microbial community analysis tools (16S rRNA gene fragment amplicon sequencing), to identify and evaluate human sewage-related impact in the Manoa watershed in Honolulu, Hawaii. Elevated concentrations of enterococci (geometric mean ranging from 1604 to 2575 CFU 100 mL −1 ) and C. perfringens (45–77 CFU 100 mL −1 ) indicated impairment of the urbanized section of the stream, while indicator bacteria concentrations decreased downstream in the tidally influenced Ala Wai Canal. The threshold values triggering water quality violation notifications in Hawaii were exceeded in 33.3–75.0% of samples collected at sites in the urbanized section of Manoa Stream, but were not exceeded in any of the samples collected at an upstream site located in a forested area. Correlation between indicator bacteria concentrations and rainfall amounts was weak to moderate but significant ( E. coli R = 0.251, P = 0.009; enterococci R = 0.369, P < 0.001; C. perfringens R = 0.343, P < 0.001), while concentrations of human fecal-associated molecular markers were not significantly correlated with rainfall (human-associated Bacteroides , R = 0.131, P = 0.256; human-associated polyomaviruses, R = 0.213, P = 0.464). Presence of human sewage was confirmed by detection of human-associated Bacteroides and human polyomavirus in the urbanized section of Manoa Stream (83.3–100% and 41.7–66.7% positive samples respectively). It was further confirmed by microbial community analyses which suggested that an average 2.4–3.4% of the total bacterial population in this section was associated with sewage. Microbial community profiles were significantly influenced by rainfall (R 2 = 0.4390, P < 0.001), pH (R 2 = 0.3077, P = 0.006), salinity (R 2 = 0.2614, P = 0.038), and conductivity (R 2 = 0.2676, P = 0.031). Although microbial diversity fluctuated throughout the watershed, it was lower in the impaired section. Leaking sewer systems and illegal cross-connections are implicated in the impairment of the watershed, hence both the sewer and the storm water lines should be routinely inspected. Collectively, our data suggest that information derived from the analysis of microbial communities complements current marker-based microbial source tracking techniques and environmental monitoring programs. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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13. Differential decomposition of bacterial and viral fecal indicators in common human pollution types.
- Author
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Wanjugi, Pauline, Sivaganesan, Mano, Korajkic, Asja, Kelty, Catherine A., McMinn, Brian, Ulrich, Robert, Harwood, Valerie J., and Shanks, Orin C.
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INDICATOR organisms in water pollution , *BACTERIAL genetics , *BIODEGRADATION , *WATER quality management , *AQUATIC microbiology , *MARINE ecology - Abstract
Understanding the decomposition of microorganisms associated with different human fecal pollution types is necessary for proper implementation of many water quality management practices, as well as predicting associated public health risks. Here, the decomposition of select cultivated and molecular indicators of fecal pollution originating from fresh human feces, septage, and primary effluent sewage in a subtropical marine environment was assessed over a six day period with an emphasis on the influence of ambient sunlight and indigenous microbiota. Ambient water mixed with each fecal pollution type was placed in dialysis bags and incubated in situ in a submersible aquatic mesocosm. Genetic and cultivated fecal indicators including fecal indicator bacteria (enterococci, E. coli , and Bacteroidales ), coliphage (somatic and F+), Bacteroides fragilis phage (GB-124), and human-associated genetic indicators (HF183/BacR287 and HumM2) were measured in each sample. Simple linear regression assessing treatment trends in each pollution type over time showed significant decay (p ≤ 0.05) in most treatments for feces and sewage (27/28 and 32/40, respectively), compared to septage (6/26). A two-way analysis of variance of log 10 reduction values for sewage and feces experiments indicated that treatments differentially impact survival of cultivated bacteria, cultivated phage, and genetic indicators. Findings suggest that sunlight is critical for phage decay, and indigenous microbiota play a lesser role. For bacterial cultivated and genetic indicators, the influence of indigenous microbiota varied by pollution type. This study offers new insights on the decomposition of common human fecal pollution types in a subtropical marine environment with important implications for water quality management applications. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
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14. Relationship of human-associated microbial source tracking markers with Enterococci in Gulf of Mexico waters
- Author
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Gordon, Katrina V., Brownell, Miriam, Wang, Shiao Y., Lepo, Joe Eugene, Mott, Joanna, Nathaniel, Rajkumar, Kilgen, Marilyn, Hellein, Kristen N., Kennedy, Elizabeth, and Harwood, Valerie J.
- Subjects
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SEWAGE disposal plants , *WATER pollution , *ECOSYSTEM health , *SALINE waters , *MEMBRANE separation , *HEALTH risk assessment , *ENTEROCOCCUS - Abstract
Abstract: Human and ecosystem health can be damaged by fecal contamination of recreational waters. Microbial source tracking (MST) can be used to specifically detect domestic sewage containing human waste, thereby informing both risk assessment and remediation strategies. Previously, an inter-laboratory collaboration developed standardized PCR methods for a bacterial, an archaeal, and a viral indicator of human sewage. Here we present results for two subsequent years of field testing in fresh and salt water by five laboratories across the U.S. Gulf Coast (two in Florida and one each in Mississippi, Louisiana and Texas) using common standard operating procedures (SOPs) developed previously. Culturable enterococci were enumerated by membrane filtration, and PCR was used to detect three MST markers targeting domestic sewage: human-associated Bacteroides (HF183), Methanobrevibacter smithii and human polyomaviruses BK and JC (HPyVs). Detection of sewage markers in surface waters was significantly associated with higher enterococci levels and with exceedance of the recreational water quality standard in four or three regions, respectively. Sewage markers were frequently co-detected in single samples, e.g., M. smithii and HF183 were co-detected in 81% of Louisiana samples, and HPyVs and M. smithii were co-detected in over 40% of southwest Florida and Mississippi samples. This study demonstrates the robustness and inter-laboratory transferability of these three markers for the detection of pollution from domestic sewage in the waters impacting the Gulf of Mexico over a coastal range of over 1000 miles. [Copyright &y& Elsevier]
- Published
- 2013
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15. Assessment of sources of human pathogens and fecal contamination in a Florida freshwater lake
- Author
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Staley, Christopher, Reckhow, Kenneth H., Lukasik, Jerzy, and Harwood, Valerie J.
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PATHOGENIC microorganisms , *FECAL contamination , *FRESHWATER ecology , *LAKES , *AQUATIC microbiology , *GENETIC markers , *SEDIMENT analysis , *BAYESIAN analysis - Abstract
Abstract: We investigated the potential for a variety of environmental reservoirs to harbor or contribute fecal indicator bacteria (FIB), DNA markers of human fecal contamination, and human pathogens to a freshwater lake. We hypothesized that submerged aquatic vegetation (SAV), sediments, and stormwater act as reservoirs and/or provide inputs of FIB and human pathogens to this inland water. Analysis included microbial source tracking (MST) markers of sewage contamination (Enterococcus faecium esp gene, human-associated Bacteroides HF183, and human polyomaviruses), pathogens (Salmonella, Cryptosporidium, Giardia, and enteric viruses), and FIB (fecal coliforms, Escherichia coli, and enterococci). Bayesian analysis was used to assess relationships among microbial and physicochemical variables. FIB in the water were correlated with concentrations in SAV and sediment. Furthermore, the correlation of antecedent rainfall and major rain events with FIB concentrations and detection of human markers and pathogens points toward multiple reservoirs for microbial contaminants in this system. Although pathogens and human-source markers were detected in 55% and 21% of samples, respectively, markers rarely coincided with pathogen detection. Bayesian analysis revealed that low concentrations (<45 CFU × 100 ml−1) of fecal coliforms were associated with 93% probability that pathogens would not be detected; furthermore the Bayes net model showed associations between elevated temperature and rainfall with fecal coliform and enterococci concentrations, but not E. coli. These data indicate that many under-studied matrices (e.g. SAV, sediment, stormwater) are important reservoirs for FIB and potentially human pathogens and demonstrate the usefulness of Bayes net analysis for water quality assessment. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
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