1. In vitro fermentation of hyaluronan by human gut microbiota: Changes in microbiota community and potential degradation mechanism.
- Author
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Pan L, Ai X, Fu T, Ren L, Shang Q, Li G, and Yu G
- Subjects
- Adult, Bacteria enzymology, Bacteria metabolism, Bacterial Proteins metabolism, Fatty Acids, Volatile analysis, Fatty Acids, Volatile metabolism, Feces microbiology, Female, Glycoside Hydrolases metabolism, Humans, Male, Polysaccharide-Lyases metabolism, Fermentation physiology, Gastrointestinal Microbiome physiology, Hyaluronic Acid metabolism
- Abstract
Hyaluronan (HA) has been widely used as a dietary supplement which can be degraded by gut microbiota. However, the interactions between HA and gut microbiota have not been fully characterized. Here, using an in vitro system, we found that HA is readily fermented by human gut microbiota but with differing fermentative activities among individuals. HA-fermentation boosted Bacteroides spp., Bifidobacterium spp., Dialister spp., Faecalibacterium spp. and produced a significant amount of acetate, propionate and butyrate. Fermentation products profiling indicated that HA could be degraded into unsaturated even-numbered and saturated odd-numbered oligosaccharides. Further, polysaccharide lyases (PLs) and glycoside hydrolases (GHs) including GH88, PL8, PL29, PL35 and PL33 were identified from B. ovatus E3, which can help to explain the structure of the fermentation products. Collectively, our study sheds new light into the metabolism of HA and forms the basis for understanding the bioavailability of HA from a gut microbiota perspective., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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