1. Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice.
- Author
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Aslam, Mohamad F., Frazer, David M., Faria, Nuno, Bruggraber, Sylvaine F. A., Wilkins, Sarah J., Mirciov, Cornel, Powell, Jonathan J., Anderson, Greg J., and Pereira, Dora I. A.
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FERRITIN ,NANOPARTICLES ,NANOMEDICAL research ,IRON in the body ,ENTEROCYTES - Abstract
The ferritin core is composed of fine nanoparticulate Fe
3+ oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe3+ poly-oxohydroxide (nanoFe3+ ). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption of the Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented with Fe2+ sulfate (FeSO4 ), nanoFe3+ , or no added Fe for a further 4 wk. A control group was Fe sufficient throughout. Direct intestinal absorption of nanoFe3+ was investigated using isolated duodenal loops. Our data show that FeSO4 and nanoFe3+ are equally bioavailable in WT mice, and at wk 8 the mean ± SEM hemoglobin increase was 18 ± 7 g/L in the FeSO4 group and 30 ± 5 g/L in the nanoFe3+ group. Oral iron failed to be utilized by Fpn-KO mice and was retained in enterocytes, irrespective of the iron source. In summary, although nanoFe3+ is taken up directly by the duodenum its homeostasis is under the normal regulatory control of dietary iron absorption, namely via ferroportin-dependent efflux from enterocytes, and thus offers potential as a novel oral iron supplement. [ABSTRACT FROM AUTHOR]- Published
- 2014
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