1. Fiber Laser-Based Lasso-Shaped Biosensor for High Precision Detection of Cancer Biomarker-CEACAM5 in Serum.
- Author
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Hu, Jie, Song, Enlai, Liu, Yuhui, Yang, Qiaochu, Sun, Junhui, Chen, Jinna, Meng, Yue, Jia, Yanwei, Yu, Zhiguang, Ran, Yang, Shao, Liyang, and Shum, Perry Ping
- Subjects
FIBER lasers ,EARLY detection of cancer ,CELL adhesion molecules ,BIOSENSORS ,CARCINOEMBRYONIC antigen ,TUMOR markers ,ELECTROCHEMILUMINESCENCE - Abstract
Detection of trace tumor markers in blood/serum is essential for the early screening and prognosis of cancer diseases, which requires high sensitivity and specificity of the assays and biosensors. A variety of label-free optical fiber-based biosensors has been developed and yielded great opportunities for Point-of-Care Testing (POCT) of cancer biomarkers. The fiber biosensor, however, suffers from a compromise between the responsivity and stability of the sensing signal, which would deteriorate the sensing performance. In addition, the sophistication of sensor preparation hinders the reproduction and scale-up fabrication. To address these issues, in this study, a straightforward lasso-shaped fiber laser biosensor was proposed for the specific determination of carcinoembryonic antigen (CEA)-related cell adhesion molecules 5 (CEACAM5) protein in serum. Due to the ultra-narrow linewidth of the laser, a very small variation of lasing signal caused by biomolecular bonding can be clearly distinguished via high-resolution spectral analysis. The limit of detection (LOD) of the proposed biosensor could reach 9.6 ng/mL according to the buffer test. The sensing capability was further validated by a human serum-based cancer diagnosis trial, enabling great potential for clinical use. The high reproduction of fabrication allowed the mass production of the sensor and extended its utility to a broader biosensing field. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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