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The present investigation on the study of colony collapse disorder revealed the presence of Nosema apis and Foulbrood in honey bee colonies as a prominent factor responsible for the colony collapse in North Indian climatic condition, nevertheless other infection causing pathogens like Varroa mite, Red ant, application of the hazardous pesticide, wax eating moth were nil in the infection carrying colony. Humanly contrived supplements such as Vitamin B Complex 15 ml, and 75 mg Oxytetracycline three times a day each in five days interval was recorded to give the phenomenal improvement in honeybee efficiency. The minimal transformation after the first medication in all the parameter were beholden after 5 days of observation, but after 2nd dose of medicine usage a significant improvement was seen in flight activity, pollen foraging efficiency, the death rate was inspected to lower upto 2-8 bees per day after 10th day of 3rd dose of medication and observation their flight activity ranges from 33-62 per 10 minute, pollen foraging efficiency was 21-56 per minute, death rate 0-2 per day and worker and queen were almost normal in health. [ABSTRACT FROM AUTHOR]