21 results on '"Cerdá-Reverter, José Miguel"'
Search Results
2. Fish Pigmentation. A Key Issue for the Sustainable Development of Fish Farming
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Cal, Laura, Suarez-Bregua, Paula, Moran, Paloma, Cerdá-Reverter, José Miguel, Rotllant, Josep, and Yúfera, Manuel, editor
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- 2018
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3. Agouti-Signalling Protein Overexpression Reduces Aggressiveness in Zebrafish.
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Rocha, Ana, Godino-Gimeno, Alejandra, Rotllant, Josep, and Cerdá-Reverter, José Miguel
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BRACHYDANIO ,ANIMAL aggression ,PROTEIN overexpression ,MELANOCORTIN receptors ,GENETIC overexpression ,FISH growth ,MIRROR images ,FOOD consumption - Abstract
Simple Summary: Enhanced feeding can be associated with aggressive behaviour since food resources are the main reason for agonistic behaviour (any social behaviour related to fighting). The overexpression of the gene agouti-signalling protein (Asip1) in transgenic zebrafish (asip1-Tg) results in enhanced food intake and linear growth. Our next question was if asip1-Tg animals exhibit a dominant phenotype associated with the feeding-enhanced levels when compared to wild-type (WT) fish. To address this question, we quantified the aggressive behaviour by conducting dyadic fights with real opponents as well as by exposing the animals to their specular image using mirrors. The results indicate that asip1-Tg are less aggressive than WT zebrafish in both dyadic fights and mirror-stimulus tests. These findings provide direct evidence of the role of the melanocortin system in the regulation of fish behaviour. The subordinate personality observed in asip1-Tg suggests that this transgene would be non-threatening to native populations in the event of an escape from aquaculture facilities. These results provide a genetic modification strategy to enhance growth in fish through high feeding motivation without promoting aggressiveness. This suggests that inhibiting the melanocortin system could be a viable target for genetically engineered fish. It is worth noting that the regulatory approval for such genetically engineered fish would be subject to the guidelines and regulations of the U.S. Food and Drug Association. Feeding motivation plays a crucial role in food intake and growth. It closely depends on hunger and satiation, which are controlled by the melanocortin system. Overexpression of the inverse agonist agouti-signalling protein (ASIP) and agouti-related protein (AGRP) leads to enhanced food intake, linear growth, and weight. In zebrafish, overexpression of Agrp leads to the development of obesity, in contrast to the phenotype observed in transgenic zebrafish that overexpress asip1 under the control of a constitutive promoter (asip1-Tg). Previous studies have demonstrated that asip1-Tg zebrafish exhibit larger sizes but do not become obese. These fish display increased feeding motivation, resulting in a higher feeding rate, yet a higher food ration is not essential in order to grow larger than wild-type (WT) fish. This is most likely attributed to their improved intestinal permeability to amino acids and enhanced locomotor activity. A relationship between high feeding motivation and aggression has been previously reported in some other transgenic species showing enhanced growth. This study aims to elucidate whether the hunger observed in asip1-Tg is linked to aggressive behaviour. Dominance and aggressiveness were quantified using dyadic fights and mirror-stimulus tests, in addition to the analysis of basal cortisol levels. The results indicate that asip1-Tg are less aggressive than WT zebrafish in both dyadic fights and mirror-stimulus tests. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Loss‐ of‐ function mutations in melanocortin-1 receptor modulate immune
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Fierro-Castro, Camino, Rotllant, Josep, Cerdá-Reverter, José Miguel, Tort, Lluis, and Ministerio de Ciencia e Innovación (España)
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Fish ,Macrophage ,Immune modulation ,MC1R ,Melanocortin receptor ,HPI - Abstract
9 `pages, 5 figures, 3 tables, The melanocortin system is an ancient neuroendocrine system conserved from teleosts to mammals. The melanocortin system is a set of complex neuroendocrine signaling pathways involved in numerous physiological processes, and particularly associated with the hypothalamic-pituitary-interrenal (HPI) axis response. The melanocortin 1 receptor (MC1R) is the central melanocortin receptor involved in pigmentation in vertebrates, including fish. In order to assess the immune role of MC1R, this study used a homozygous Mc1r knockout zebrafish. Hence, skin cortisol levels, variations in the blood leucocyte population, as well as the expression levels of immune genes in various tissues of wild-type TU strain (Tübingen, Nüsslein-Volhard Lab) (WT) and homozygous mc1r knockout zebrafish (mc1rK.O.) stimulated with LPS was carried out. Results show that the mc1rK.O. mutant fish produce lower levels of cortisol in mucus and fewer macrophages in blood after exposure to LPS compared to control fish. Regarding the expression of immune genes, mutant fish show a significant increase in the expression of the anti-inflammatory interleukin il10. These results suggest that the mc1rK.O. mutant fish may follow an alternative mechanism among the immune responses, where macrophages seem to have an anti-inflammatory function, attenuating nitric oxide (NO) production and providing an advantage through the mitigation of excessive or strong inflammatory reactions. Nonetheless, a lower number of this cell type could imply a reduced phagocytic potential in the face of an infection. At the same time, lower cortisol levels in the mc1rK.O. mutant fish could be an advantage as for the lower susceptibility to stress and the physiological and metabolic consequences of high cortisol levels, Part of this work was funded by grant number AGL2017-89648P from the MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe
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- 2022
5. Role of the Melanocortin System in Gonadal Steroidogenesis of Zebrafish
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Navarro, Sandra, Crespo, Diego, Schulz, Rüdiger W, Ge, Wei, Rotllant, Josep, Cerdá-Reverter, José Miguel, Rocha, Ana, Sub Developmental Biology, Developmental Biology, Agencia Estatal de Investigación (España), Fundação para a Ciência e a Tecnologia (Portugal), Sub Developmental Biology, and Developmental Biology
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fish ,General Veterinary ,agouti-signaling protein ,POMC ,veterinary(all) ,spermatogenesis ,ovarian follicles ,reproduction ,ASIP ,MSH ,Animal Science and Zoology ,Conserve and sustainably use the oceans, seas and marine resources for sustainable development - Abstract
17 pages, 2 tables, 5 figures.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, In teleost, as in other vertebrates, stress affects reproduction. A key component of the stress response is the pituitary secretion of the adrenocorticotropic hormone (ACTH), which binds to the melanocortin 2 receptor (MC2R) in the adrenal glands and activates cortisol biosynthesis. In zebrafish, Mc2r was identified in male and female gonads, while ACTH has been shown to have a physiological role in modulating reproductive activity. In this study, the hypothesis that other melanocortins may also affect how the zebrafish gonadal function is explored, specifically steroid biosynthesis, given the presence of members of the melanocortin signaling system in zebrafish gonads. Using cell culture, expression analysis, and cellular localization of gene expression, our new observations demonstrated that melanocortin receptors, accessory proteins, antagonists, and agonists are expressed in both the ovary and testis of zebrafish (n = 4 each sex). Moreover, melanocortin peptides modulate both basal and gonadotropin-stimulated steroid release from zebrafish gonads (n = 15 for males and n = 50 for females). In situ hybridization in ovaries (n = 3) of zebrafish showed mc1r and mc4r in follicular cells and adjacent to cortical alveoli in the ooplasm of previtellogenic and vitellogenic oocytes. In zebrafish testes (n = 3), mc4r and mc1r were detected exclusively in germ cells, specifically in spermatogonia and spermatocytes. Our results suggest that melanocortins are, directly or indirectly, involved in the endocrine control of vitellogenesis in females, through modulation of estradiol synthesis via autocrine or paracrine actions in zebrafish ovaries. Adult zebrafish testes were sensitive to low doses of ACTH, eliciting testosterone production, which indicates a potential role of this peptide as a paracrine regulator of testicular function., This research was supported by Spanish State Agency of Research (AEI), grant number AGL2016-74857-C3-3-R and PID2019-103969RB-C33 to JMCR and AGL2017-89648P to JR, Portuguese national funds by FCT-Foundation for Science and Technology through the projects PTDC/CVT-CVT/3205/2020, UIDB/04423/2020 and UIDP/04423/2020 and PTDC/CVT-CVT/3205/2020 to AR and National Agency for Research and Development (ANID), Scholarship Program, DOCTORADO BECAS CHILE fellowship 2013–72140242 to SN
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- 2022
6. Characterization of sea bass FSHβ 5′ flanking region: transcriptional control by 17β-estradiol
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Muriach, Borja, Carrillo, Manuel, Zanuy, Silvia, and Cerdá-Reverter, José Miguel
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- 2014
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7. Central regulation of food intake in fish: an evolutionary perspective.
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Luis Soengas, José, Cerdá-Reverter, José Miguel, and Jesús Delgado, María
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FISH evolution , *OSTEICHTHYES , *FOOD consumption , *CONSERVED sequences (Genetics) , *HOMOLOGY (Biochemistry) - Abstract
Evidence indicates that central regulation of food intake is well conserved along the vertebrate lineage, at least between teleost fish and mammals. However, several differences arise in the comparison between both groups. In this review, we describe similarities and differences between teleost fish and mammals on an evolutionary perspective. We focussed on the existing knowledge of specific fish features conditioning food intake, anatomical homologies and analogies between both groups as well as the main signalling pathways of neuroendocrine and metabolic nature involved in the homeostatic and hedonic central regulation of food intake. [ABSTRACT FROM AUTHOR]
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- 2018
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8. Transcriptional regulation of Aanat-2 and Hiomt-2 in goldfish
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Velarde, Elena, Cerdá-Reverter, José Miguel, Alonso-Gómez, Ángel Luis, Sánchez, Elena, Isorna, Esther, and Delgado, María Jesús
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Transcriptional regulation ,Pineal ,Fish ,Liver ,Arylalkylamine N-acetyltransferase ,Gut ,sense organs ,Hydroxyindole-O-methyltransferase ,Retina - Abstract
24 p., il. y bibliografía Melatonin is currently proposed to be synthesized in non-photosensitive organs of vertebrates, besides its well-known sites of synthesis, the pineal gland and the retina. However, very few studies have demonstrated gene expression of MEL synthesizing enzymes in extrapineal and extraretinal locations. In the present study, present study focuses on the circadian expression of the two key enzymes of the melatoninergic pathway, the AANAT and the HIOMT in central and peripheral locations of the goldfish we give report of the full-length cloning of the two enzymes catalyzing the final steps of melatonin biosynthesis, the arylalkylamine N-acetyltransferase (AANAT) and the hydroxyindole-O-methyltransferase (HIOMT), in a teleosts fish, the goldfish (Carassius auratus). Both enzymes showed high similarity with other teleost sequences, corresponding to the goldfish AANAT-2 and HIOMT-2. Two forms of AANATs were widely known to exist in teleosts, but this is the first time that two isoforms of HIOMT are deduced from a phylogenetic analysis. Both enzymes were detected in several peripheral locations, including liver and gut, being present results the first to find HIOMT in non-photosensitive structures of a fish species. No studies exist on transcriptional regulation of the expression of MEL biosynthesis enzymes in non photosensitive structures in fish The daily expression pattern of both genes in pineal gland, retina, liver and gut was investigated using quantitative real time RT-PCR and cosinor analysis. This is the first time that a rhythmic expression of AANAT and HIOMT are found in digestive tissues of a vertebrate species, supporting a functional melatonin synthesis pathway in liver and gut of the goldfish. Besides, the transcriptional regulation of Aanat-2 in pineal and peripheral locations of goldfish maintained under different lighting conditions was investigated expression of gAANAT-2 is analyzed under different lighting conditions including continuous light and darkness, revealing, as expected, light-dependent rhythms in pineal gland and retina, but also in liver. Nevertheless, the persistence in hindgut of these Aanat-2 rhythms in constant conditions suggests that the expression of this transcript is under circadian clock and entrained by non-photic cues. Our results reinforce the existence of melatonin synthesis in gut and liver of the goldfish, while the rhythmic expression profiles reported point to a regulation of both genes in gut and liver by peripheral oscillators entrainable to non-photic cues. Supported by the Spanish Ministry of Science and Innovation (AGL2007-65744-C03-03 to MJD and AGL2007-65744-C03-02 to JMC-R) and by BSCH-UCM (GR58/08-A). E. Velarde is a predoctoral fellow from the Ministry of Science and Innovation and the European Social Fund.
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- 2010
9. Cytoarchitectonic study of the brain of a perciform species, the sea bass (Dicentrarchus labrax). II The diencephalon
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Cerdá-Reverter, José Miguel, Zanuy, Silvia, Muñoz-Cueto, José Antonio, Fundación Balaguer Gonel Hermanos, Comisión Interministerial de Ciencia y Tecnología, CICYT (España), and European Commission
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Neuroanatomy ,Fish ,Teleost ,Brain ,Cytoarchitecture ,Diencephalon - Abstract
The cytoarchitecture of nuclei in the preoptic area, ventral thalamus, dorsal thalamus, epithalamus, hypothalamus, posterior tuberculum, synencephalon, and pretectum and the accessory optic nuclei was analyzed in a perciform teleost, the sea bass Dicentrarchus labrax, by using serial sections stained with cresyl-violet. In general, the cytoarchitecture of the preoptic area, ventral and dorsal thalamus, epithalamus, and synencephalon resembles the histological pattern of other teleosts. However, the parvocellular preoptic nucleus of sea bass has been subdivided into parvocellular and anteroventral parts for morphological and functional reasons. The hypothalamus of the sea bass seems to differ slightly from that of other teleosts. An elaborated lateral tuberal nucleus, with five subdivisions, and three different nuclei around the lateral recesses were recognized. A medial nucleus of the inferior lobe, which has been reported previously in the perciform Sparus aurata, is also present in the hypothalamus of sea bass but has not been described before in another advanced teleost. The organization of the pretectum and the accessory optic system is essentially similar in sea bass to that described in other perciforms with highly developed vision. The migrated portion of the posterior tuberculum of sea bass appears to differ from this region of the diencephalon in other teleosts. In sea bass, three cell masses that have been described previously only in the perciform Sparus aurata have been assigned to the migrated area of the posterior tuberculum. This study will provide the neuroanatomical basis for future morpho-functional studies to be done in the sea bass brain., J.M.C-R was a recipient ofa fellowship from Balaguer-Gonel Foundation. Contract grant sponsor: CICYT; Contract grant number: CYTMARMAR95-1888-C03-02; Contract grant sponsor: UE; Contract grantnumber: FAIR CT97-3785
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- 2001
10. Time-course studies on plasma glucose, insulin, and cortisol in sea bass (Dicentrarchus labrax) held under different photoperiodic regimes
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Cerdá-Reverter, José Miguel, Zanuy, Silvia, Carrillo, Manuel, Madrid, Juan Antonio, and European Commission
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Blood Glucose ,endocrine system ,medicine.medical_specialty ,Hydrocortisone ,medicine.medical_treatment ,Photoperiod ,Experimental and Cognitive Psychology ,Daily rhythms ,Cortisol ,Eating ,Behavioral Neuroscience ,Feeding behavior ,Internal medicine ,medicine ,Animals ,Insulin ,Circadian rhythm ,Sea bass ,Pancreatic hormone ,photoperiodism ,biology ,digestive, oral, and skin physiology ,biology.organism_classification ,Food restriction ,Endocrinology ,Fish ,Glucose ,Bass ,Dicentrarchus ,Energy Metabolism ,Food Deprivation ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug ,Hormone - Abstract
Daily variations of insulin, cortisol, and glucose are studied in animals adapted to two different photoperiodic regimes, with the intervals between feeding times and photoperiodic events kept constant. Data support the existence of a daily rhythm of plasma glucose which seems to be photoperiodic. In contrast, the daily patterns of insulin in sea bass seem to be mainly influenced by feeding time; however, an effect of photoperiod can not be excluded. When the digestive tract is absent of food, insulin levels are generally minimal at feeding times and maximal during the inter-meals periods, suggesting the central control of insulin secretion during short-term food deprivation. Contrarily, the nadir values of plasma cortisol were reached at midday during the inter-meal period and peak plasma levels were evident at both light onset and offset. Disruption between metabolite and hormone patterns suggest that they are under different controls. Such results could be explained under the existence of a multioscillator system, including a food entrainable oscillator in addition to the master light entrainable oscillator., This research was supported by European Commission Project no: FAR Project AQ 2417. The authors wish to thank J. Gutiérrez and W. Hanke, who were also involved in EC FAR Project AQ. 2417, for kindly providing their facilities for the insulin and cortisol assays, respectively. We are also grateful to L. A. Sorbera for critical review of the manuscript and to J. Ramos, M. Blázquez, and X. Simó for their immense help in fish sampling.
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- 1998
11. Ghrelin modulates hypothalamic fatty acid-sensing and control of food intake in rainbow trout.
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Velasco, Cristina, Librán-Pérez, Marta, Otero-Rodiño, Cristina, López-Patiño, Marcos A, Mıguez, Jesús M, Cerdá-Reverter, José Miguel, and Soengas, José L
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GHRELIN ,HYPOTHALAMIC hormones ,PHYSIOLOGICAL effects of fatty acids ,INGESTION ,RAINBOW trout - Abstract
There is no information available on fish as far as the possible effects of ghrelin on hypothalamic fatty acid metabolism and the response of fatty acid-sensing systems, which are involved in the control of food intake. Therefore, we assessed in rainbow trout the response of food intake, hypothalamic fatty acid-sensing mechanisms and expression of neuropeptides involved in the control of food intake to the central treatment of ghrelin in the presence or absence of a long-chain fatty acid such as oleate. We observed that the orexigenic actions of ghrelin in rainbow trout are associated with changes in fatty acid metabolism in the hypothalamus and an inhibition of fatty acid-sensing mechanisms, which ultimately lead to changes in the expression of anorexigenic and orexigenic peptides resulting in increased orexigenic potential and food intake. Moreover, the response to increased levels of oleate of hypothalamic fatty acid-sensing systems (activation), expression of neuropeptides (enhanced anorexigenic potential) and food intake (decrease) were counteracted by the simultaneous treatment with ghrelin. These changes provide evidence for the first time in fish of a possible modulatory role of ghrelin on the metabolic regulation by fatty acid of food intake occurring in the hypothalamus. [ABSTRACT FROM AUTHOR]
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- 2016
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12. A cytoarchitectonic study of the brain of a perciform species, the sea bass (Dicentrarchus labrax): The midbrain and hindbrain
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Cerdá-Reverter, José Miguel, Muriach, Borja, Zanuy, Silvia, and Muñoz-Cueto, José Antonio
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SEA basses , *BRAIN stem , *EUROPEAN seabass , *MORPHOLOGY - Abstract
Summary: This study is the third part of a comprehensive series of publications on the cytoarchitectonic organization of the brain of the European sea bass, Dicentrarchus labrax. This study provides an atlas of the brain stem based on Nissl-stained transverse sections as well as a description of cell masses and a discussion on comparative aspects of brain stem nuclei, including methodological studies in other species. By external examination, the sea bass exhibits a prominent Optic tectum and Corpus cerebelli as expected in a predator species with a highly developed visual system. However, no hypertrophy of the facial and vagal lobes was observed as reported in other non-perciform teleosts. The general organization pattern of the mesencephalon and rhombencephalon of the sea bass brain resembles that reported for other perciform teleosts. However, the Valvula cerebelli has been subdivided into anterior, central and posterior parts. In addition, the ventricular surface of the granular layer of the Valvula cerebelli appears to be in contact with those of the Torus longitudinalis. This cell apposition could be interpreted as a direct connection, but more studies demonstrating the absence of ependyma between both structures are needed. Furthermore, we have tentatively described the electro/mechano receptive pre-eminential nucleus in the rhombencephalon of the sea bass. This study completes one of the few descriptions, as well as the most complete and detailed available, of the brain of any marine perciform species. [Copyright &y& Elsevier]
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- 2008
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13. Molecular characterization and central distribution of the estradiol receptor alpha (ERα) in the sea bass (Dicentrarchus labrax)
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Muriach, Borja, Cerdá-Reverter, José Miguel, Gómez, Ana, Zanuy, Silvia, and Carrillo, Manuel
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SEA basses , *ESTRADIOL , *GONADOTROPIN , *IN situ hybridization - Abstract
Abstract: Three different estrogen receptors (ERs) have been cloned and characterized in teleosts fish, i.e. ERα, ERβ or ERβ1 and ERγ or ERβ2. In order to study the sea bass ER subtype involved in the regulation of gonadotropin production, as well as to elucidate the possible involved neuronal pathways, we characterized the transactivation properties of the cloned sea bass ERα (sbERα) and studied its distribution in the brain and gonadotropic cells of the sea bass by in situ hybridization. The results revealed that sbERα transactivates promoters containing estradiol responsive elements (ERE) in a dose–response manner. The sbERα showed the highest affinity for 17-β-estradiol. In situ hybridization studies demonstrated that ERα mRNA positive neurons are widely distributed within the sea bass brain, including the telencephalon, preoptic area, thalamus, hypothalamus, mesencephalic tectum and tegmentum and rhombencephalon. New estrogen dependent nuclei were described in all above areas. The sbERα was profusely expressed in the main neuroendocrine areas such as the preoptic area and hypothalamus, thus suggesting the steroidal modulation of the hypophysiotropic neurons. The presence of sbERα expression in the FSHβ and LHβ cells suggests a direct effect of estrogens in the control of gonadotropin hormone synthesis. [Copyright &y& Elsevier]
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- 2008
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14. In situ localization of preprogalanin mRNA in the goldfish brain and changes in its expression during feeding and starvation
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Unniappan, Suraj, Cerdá-Reverter, José Miguel, and Peter, Richard E.
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GALANIN , *BRAIN , *FISHES , *MESSENGER RNA - Abstract
In this paper, we report (i) the in situ localization, and (ii) meal time related and starvation induced changes in preprogalanin mRNA expression in the goldfish brain. The specific brain nuclei that express galanin mRNA are the area ventralis telencephali pars ventralis, nucleus preopticus periventricularis, nucleus lateralis tuberis, and the nucleus recessus lateralis. No changes in preprandial preprogalanin mRNA expression were found in the brain regions studied. No changes in postprandial preprogalanin mRNA expression were found compared to the preprandial levels. However, in unfed fish, a significant increase in preprogalanin mRNA expression was found in the telencephalon (1 and 3 h) and hypothalamus (3 h) after the scheduled feeding time. The postprandial preprogalanin mRNA expression in the telencephalon and hypothalamus of fed fish at 1 and 3 h were significantly lower than the mRNA expression levels in the unfed fish at the same time. Preprogalanin mRNA expression levels remain unaltered during 7 days of starvation. The presence of preprogalanin mRNA in brain nuclei involved in the regulation of food intake and pituitary hormone secretion suggests important physiological roles for galanin in goldfish. [Copyright &y& Elsevier]
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- 2004
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15. Brain transcriptome profile after CRISPR-induced ghrelin mutations in zebrafish
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José Miguel Cerdá-Reverter, A.I. Valenciano, María Jesús Delgado, Lucia Soletto, Raúl Cortés, Elisa Sánchez, Ayelén Melisa Blanco, Juan Ignacio Bertucci, Ministerio de Economía y Competitividad (España), Ministerio de Educación y Ciencia (España), Cerdá-Reverter, José Miguel, and Cerdá-Reverter, José Miguel [0000-0003-1405-5750]
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Physiology ,RNA-Seq ,Aquatic Science ,Biology ,Biochemistry ,Transcriptome ,03 medical and health sciences ,CRISPR ,Gene silencing ,Animals ,Clustered Regularly Interspaced Short Palindromic Repeats ,Zebrafish ,CRISPR/Cas9 ,030304 developmental biology ,Ghrelinergic system ,0303 health sciences ,Gene knockdown ,Oxygen transport ,Brain ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Ghrelin ,Cell biology ,MRNA Sequencing ,Fish ,Transcriptomic ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Knockdown ,RNA-seq - Abstract
Ghrelin (GRL) is a gut-brain hormone with a role in a wide variety of physiological functions in mammals and fish, which points out the ghrelinergic system as a key element for the appropriate biological functioning of the organism. However, many aspects of the multifunctional nature of GRL remain to be better explored, especially in fish. In this study, we used the CRISPR/Cas9 genome editing technique to generate F0 zebrafish in which the expression of grl is compromised. Then, we employed high-throughput mRNA sequencing (RNA-seq) to explore changes in the brain transcriptome landscape associated with the silencing of grl. The CRISPR/Cas9 technique successfully edited the genome of F0 zebrafish resulting in individuals with considerably lower levels of GRL mRNAs and protein and ghrelin O-acyl transferase (goat) mRNAs in the brain, intestine, and liver compared to wild-type (WT) zebrafish. Analysis of brain transcriptome revealed a total of 1360 differentially expressed genes (DEGs) between the grl knockdown (KD) and WT zebrafish, with 664 up- and 696 downregulated DEGs in the KD group. Functional enrichment analysis revealed that DEGs are highly enriched for terms related to morphogenesis, metabolism (especially of lipids), entrainment of circadian clocks, oxygen transport, apoptosis, and response to stimulus. The present study offers valuable information on the central genes and pathways implicated in functions of GRL, and points out the possible involvement of this peptide in some novel functions in fish, such as apoptosis and oxygen transport., This study was supported by a grant from the Spanish Ministry of Economy and Competitiveness (MINECO; AGL2016-74857-C3-2-R and AGL2016-74857-C3-3-R) to MJD and JMCR. AMB was a predoctoral fellow funded by the Spanish Ministry of Education and Science when this study was carried out.
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- 2019
16. Hypothalamic Integration of Metabolic, Endocrine, and Circadian Signals in Fish: Involvement in the Control of Food Intake
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María Jesús Delgado, José L. Soengas, José Miguel Cerdá-Reverter, Ministerio de Economía y Competitividad (España), European Commission, Cerdá-Reverter, José Miguel [0000-0003-1405-5750], Soengas, José L. [0000-0002-6847-3993], Cerdá-Reverter, José Miguel, and Soengas, José L.
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0301 basic medicine ,Cart ,Leptin ,nutrient sensing ,circadian rhythm ,medicine.medical_specialty ,food intake ,Biología ,Population ,Circadian clock ,Hypothalamus ,Review ,Biology ,leptin ,lcsh:RC321-571 ,03 medical and health sciences ,Food intake ,Internal medicine ,medicine ,Circadian rhythm ,hypothalamus ,education ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Fisiología animal ,fish ,education.field_of_study ,Nutrient sensing ,General Neuroscience ,digestive, oral, and skin physiology ,Neuropeptide Y receptor ,Ghrelin ,030104 developmental biology ,Endocrinology ,Fish ,nervous system ,ghrelin ,Peces ,hormones, hormone substitutes, and hormone antagonists ,Neuroscience - Abstract
The regulation of food intake in fish is a complex process carried out through several different mechanisms in the central nervous system (CNS) with hypothalamus being the main regulatory center. As in mammals, a complex hypothalamic circuit including two populations of neurons: one co-expressing neuropeptide Y (NPY) and Agouti-related peptide (AgRP) and the second one population co-expressing pro-opiomelanocortin (POMC) and cocaine- and amphetamine-regulated transcript (CART) is involved in the integration of information relating to food intake control. The production and release of these peptides control food intake, and the production results from the integration of information of different nature such as levels of nutrients and hormones as well as circadian signals. The present review summarizes the knowledge and recent findings about the presence and functioning of these mechanisms in fish and their differences vs. the known mammalian model, The authors acknowledge grants from Spanish Agencia Estatal de Investigación (AEI) and European Fund of Regional Development (FEDER) to MD (AGL2016-74857-C3-2-R), JC (AGL2016-74857-C3-3-R), and JS (AGL2016-74857-C3-1-R).
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- 2016
17. Fish pigmentation and the melanocortin system.
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Cal, Laura, Suarez-Bregua, Paula, Rotllant, Josep, Cerdá-Reverter, José Miguel, and Braasch, Ingo
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MELANOCORTIN receptors , *NEUROENDOCRINE system , *FISH pigments , *AGOUTI-related peptide , *MELANOSOMES - Abstract
The melanocortin system is a complex neuroendocrine signaling mechanism involved in numerous physiological processes in vertebrates, including pigmentation, steroidogenesis and metabolic control. This review focuses at one of its most fascinating function in fish, its regulatory role in the control of pigmentation, in which the melanocortin 1 receptor (Mc1r), its agonist α-melanocyte stimulating hormone (α-Msh), and the endogenous antagonist agouti signaling protein (Asip1) are the main players. Functional control of Mc1r, which is highly expressed in fish skin and whose activation stimulates melanin production and melanosome dispersion in fish melanophores, is considered a key mechanism for vertebrate pigment phenotypes. The α-Msh peptide, the most documented Mc1r agonist involved in pigmentation, is produced in the pituitary gland, activating melanin synthesis by binding to Mc1r in fish melanophores. Finally, Asip1 is the putative factor for establishing the evolutionarily conserved dorso-ventral pigment pattern found across vertebrates. However, we are just starting to understand how other melanocortin system components are acting in this complex regulatory network. [ABSTRACT FROM AUTHOR]
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- 2017
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18. Characterization, tissue distribution and regulation by fasting of the agouti family of peptides in the sea bass (Dicentrarchus labrax).
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Agulleiro, Maria Josep, Cortés, Raúl, Leal, Esther, Ríos, Diana, Sánchez, Elisa, and Cerdá-Reverter, José Miguel
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AGOUTI-related peptide , *MELANOCORTIN receptors , *CELLULAR signal transduction , *FISH endocrinology , *CHROMOSOME duplication , *IMMUNOCYTOCHEMISTRY , *SEA basses , *FISHES - Abstract
The melanocortin system is one of the most complex hormonal systems in vertebrates. Atypically, the signaling of melanocortin receptors is regulated by the binding of endogenous antagonists, named agouti-signaling protein (ASIP) and agouti-related protein (AGRP). Teleost specific genome duplication (TSGD) rendered new gene copies in teleost fish and up to four different genes of the agouti family of peptides have been characterized. In this paper, molecular cloning was used to characterize mRNA of the agouti family of peptides in sea bass. Four different genes were identified: AGRP1, ASIP1, AGRP2 and ASIP2. The AGRP1 gene is mainly expressed in the brain whereas ASIP1 is mainly expressed in the ventral skin. Both ASIP2 and AGRP2 are expressed in the brain and the pineal gland but also in some peripheral tissues. Immunocytochemical studies demonstrated that AGRP1 is exclusively expressed within the lateral tuberal nucleus, the homologue of the mammalian arcuate nucleus in fish. Long-term fasting (8–29 days) increased the hypothalamic expression of AGRP1 but depressed AGRP2 expression (15–29 days). In contrast, the hypothalamic expression of ASIP2 was upregulated during short-term fasting suggesting that this peptide could be involved in the short term regulation of food intake in the sea bass. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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19. Effects of dopaminergic system activation on feeding behavior and growth performance of the sea bass (Dicentrarchus labrax): A self-feeding approach.
- Author
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Leal, Esther, Fernández-Durán, Begoña, Agulleiro, Maria Josep, Conde-Siera, Marta, Míguez, Jesús Manuel, and Cerdá-Reverter, José Miguel
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DOPAMINERGIC mechanisms , *SEA basses , *ANIMAL feeding behavior , *GROWTH , *ANIMAL morphology , *DOPA , *ADRENALINE - Abstract
Abstract: Dopamine is synthesized from l-dopa and subsequently processed into norepinephrine and epinephrine. Any excess neurotransmitter can be taken up again by the neurons to be broken down enzymatically into DOPAC. The effect of dopamine on mammalian food intake is controversial. Mice unable to synthesize central dopamine die of starvation. However, studies have also shown that central injection of dopamine inhibits food intake. The effect of dopaminergic system in the fish feeding behavior has been scarcely explored. We report that the inclusion of l-dopa in the diets results in the activation of sea bass central dopaminergic system but also in the significant increase of the hypothalamic serotonin levels. Dietary l-dopa induces a decrease of food intake and feed conversion efficiency that drives a decline of all growth parameters tested. No behavioral effects were observed after l-dopa treatment. l-dopa treatment stimulated central expression of NPY and CRF. It suggests that CRF might mediate l-dopa effects on food intake but also that CRF neurons lie downstream of NPY neurons in the hierarchical forebrain system, thus controlling energy balance. Unexpectedly, dietary administration of haloperidol, a D2-receptor antagonist, cannot block dopamine effects but also induces a decline of the food intake. This decrease seems to be a side effect of haloperidol treatment since fish exhibited a decreased locomotor activity. We conclude that oral l-dopa inhibits sea bass food intake and growth. Mechanism could also involve an increase of hypothalamic serotoninergic tone. [Copyright &y& Elsevier]
- Published
- 2013
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20. Role of melanocortin receptor accessory proteins in the function of zebrafish melanocortin receptor type 2
- Author
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Agulleiro, Maria Josep, Roy, Simon, Sánchez, Elisa, Puchol, Sara, Gallo-Payet, Nicole, and Cerdá-Reverter, José Miguel
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PEPTIDE hormones , *ZEBRA danio , *GENE expression , *ADIPOSE tissues , *IMMUNOFLUORESCENCE , *CELL membranes , *ADRENOCORTICOTROPIC hormone - Abstract
Abstract: In this paper, we identify three different MRAPs in zebrafish, zfMRAP1, zfMRAP2a and zfMRAP2b, and demonstrate that zfMC2R is not functional in the absence of MRAP expression. ZfMRAP1 expression was restricted to adipose tissue and the anterior kidney whereas MRAP2a and MRAP2b were expressed in all the tissues tested. Quantification of surface receptor and immunofluorescence studies indicated that the receptor is unable to translocate to membrane in the absence of MRAP isoforms. MRAP1 and MRAP2b are localized in the plasma membrane in the absence of zfMC2R expression but MRAP2b is retained in perinuclear position. MRAP1 and MRAP2a displayed an equivalent translocation capacity to the membrane of zfMC2R but only zfMRAP1 expression led to intracellular cAMP increases after ACTH stimulation. ZfMRAP2b had no effect on zfMC2R activity but both zfMRAP2 isoforms enhanced the zfMRAP1-assisted cAMP intracellular increase, suggesting an interaction between zfMRAP1 and zfMRAP2s when regulating zfMC2R activity. [Copyright &y& Elsevier]
- Published
- 2010
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21. Distribution of estrogen receptor 2 mRNAs (Esr2a and Esr2b) in the brain and pituitary of the sea bass (Dicentrarchus labrax)
- Author
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Muriach, Borja, Carrillo, Manuel, Zanuy, Silvia, and Cerdá-Reverter, José Miguel
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ESTROGEN receptors , *LUTEINIZING hormone , *GLYCOPROTEIN hormones , *GONADOTROPIN , *PITUITARY hormones , *SEA basses , *PHYSIOLOGY - Abstract
Abstract: Three different estrogen receptors (ERs) have been characterized in teleost fish, i.e. Esr1, Esr2a and Esr2b. In this study we carried out in situ hybridizations in the brain and pituitary of the sea bass (Dicentrarchus labrax) to study the putative involvement of Esr2 subtypes in the control of gonadotropin secretion in fish. Our studies demonstrated that both receptors are expressed within the main hypophysiotrophic areas of the sea bass brain thus providing neuroanatomical basis for the involvement of Esr2 subtypes in the long (or indirect) regulatory feedbacks on pituitary function in the sea bass. The results revealed that Esr2b-mRNA distribution was restricted to the preoptic area and tuberal hypothalamus. On the contrary, Esr2a presented a widespread distribution and transcripts were detected within the ventral telencephalon, preoptic area, hypothalamus, thalamus, posterior tubercle, mesencephalon and rhombencephalon. New Esr2-expressing areas were described in all of the above areas. This paper is the first demonstration of Esr2a and Esr2b expression in the follicle-stimulating hormone β-subunit (βFSH)- and luteinizing hormone β-subunit (βLH)-expressing cells in the fish pituitary, thus suggesting the participation of both receptors in the direct effect of estrogen on the control of gonadotropin hormone synthesis. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
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