12 results on '"Montfort, Jérôme"'
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2. Transcriptomic profiling of eggs of pikeperch (Sander lucioperca) reveals novel egg-quality-associated transcripts
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Żarski, D., Le Cam, Aurélie, Nynca, J., Klopp, Christophe, Ciesielski, S., Montfort, Jérôme, Fontaine, Pascal, Ciereszko, A., Bobe, Julien, Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de la Recherche Agronomique (INRA), Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), This work was funded by National Science Center of Poland (HARMONIA project No. UMO-2016/22/M/NZ9/00590)., European Aquaculture Society (EAS). BEL., Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), and Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA)
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fish ,qualité des gamètes ,qualité des oeufs ,[SDV]Life Sciences [q-bio] ,reproduction ,oeuf de poisson ,lucioperca lucioperca ,poisson ,aquaculture ,walleye (fish) ,embryonic structures ,percidae ,transcriptome ,sandre ,expression des gènes - Abstract
Transcriptomic profiling of eggs of pikeperch (Sander lucioperca) reveals novel egg-quality-associated transcripts. Aquaculture Europe 2019
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- 2019
3. Transcriptional rewiring, post-transcriptional regulation and neo-functionalization: how the master sex-determining gene of medaka was born
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Herpin, Amaury, Schmidt, Cornelia, Kneitz, Susanne, Gobe, Clara, Regensburger, Martina, Le Cam, Aurélie, Montfort, Jérôme, Adolfi, Mateus C., Lillesaar, Christina, Kraeussling, Michael, Mourot, Brigitte, Porcon, Béatrice, Pannetier, Maelle, Pailhoux, Eric, Ettwiller, Laurence, Dolle, Dirk, Guiguen, Yann, Schartl, Manfred, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Physiological Chemistry, Biocenter, Julius-Maximilians-Universität Würzburg [Wurtzbourg, Allemagne] (JMU), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Centre for Organismal Studies (COS), Department of developmental Biology, Heidelberg University, Comprehensive Cancer Center Mainfranken, Leibniz Institute of Freshwater Ecology and Inland Fisheries (IGB). DEU., Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and University of Heidelberg
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fish ,détermination du sexe ,medaka ,[SDV]Life Sciences [q-bio] ,education ,gène déterminant majeur du sexe ,sex determination ,oryzias latipes ,reproduction ,poisson ,gène dmrt1bY ,adrianichthyidae ,gonadogénèse ,transcription ,expression des gènes - Abstract
International audience; Transcriptional rewiring, post-transcriptional regulation and neo-functionalization: how the master sex-determining gene of medaka was born. Workshop: Paradigm shift in sex chromosome evolution.
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- 2019
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- View/download PDF
4. Functional and proteomic characterisation of salmonid coelomic fluid
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Guého, Aurélie, Rime, Hélène, Com, Emmanuelle, Lavigne, Régis, Guevel, Blandine, Montfort, Jérôme, Pineau, Charles, Bobe, Julien, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), U1085 - Plate-forme protéomique de Biogenouest (PROTIM), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), ANR grant # 16-CE20-001 EggPreserve, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR), European Aquaculture Society (EAS). BEL., Corvaisier, Maryse, Iddentification de protéines du fluide ovarien impliquées dans la préservation de la qualité des oeufs de poisson - - EggPreserve2016 - ANR-16-CE20-0001 - AAPG2016 - VALID, Proteomics Core Facility (Protim), Université de Rennes (UR)-Plateforme Génomique Santé Biogenouest®, ANR-16-CE20-0001,EggPreserve,Iddentification de protéines du fluide ovarien impliquées dans la préservation de la qualité des oeufs de poisson(2016), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Plateforme Génomique Santé Biogenouest®, and ProdInra, Archive Ouverte
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gamete quality ,[SDV]Life Sciences [q-bio] ,nature conservation ,hplc ,approche protéomique ,reproduction ,oeuf de poisson ,poisson ,rainbow ,salmonids ,viabilité ,fish reproduction ,liquide coelomique ,ComputingMilieux_MISCELLANEOUS ,high pressure liquid chromatography ,mass spectrometry ,fish ,salmonidae ,trout ,oncorhynchus mykiss ,viability ,conservation ,rainbow trout ,[SDV] Life Sciences [q-bio] ,aquaculture ,mécanisme biologique ,coelomic fluid ,truite arc en ciel - Abstract
International audience
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- 2019
5. MiR-202 controls female fecundity by regulating medaka oogenesis
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Gay, Stéphanie, Bugeon, Jérôme, Bouchareb, Amine, Henry, Laure, Delahaye, Clara, Legeai, Fabrice, Montfort, Jérôme, Le Cam, Aurélie, Siegel, Anne, Bobe, Julien, Thermes, Violette, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Centre National de la Recherche Scientifique (CNRS), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Scalable, Optimized and Parallel Algorithms for Genomics (GenScale), Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-CentraleSupélec-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Dynamics, Logics and Inference for biological Systems and Sequences (Dyliss), ANR-16-COFA-0004, Agence Nationale de la Recherche, ANR-11-INBS-0014,TEFOR,Transgenèse pour les Etudes Fonctionnelles sur les Organismes modèles(2011), ANR-16-COFA-0004,AquaCrispr,Optimization of the CRISPR/Cas9 knock-in technology and application in salmon and trout(2016), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), and ANR-11-INBS-0014/11-INBS-0014,TEFOR,Transgenèse pour les Etudes Fonctionnelles sur les Organismes modèles(2011)
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Male ,MiR-202 ,Physiology ,[SDV]Life Sciences [q-bio] ,Oryzias ,Biochemistry ,Epithelium ,Animals, Genetically Modified ,Gene Knockout Techniques ,oeuf de poisson ,Oogenesis ,poisson ,Animal Cells ,Reproductive Physiology ,Medicine and Health Sciences ,Gene Editing ,Obstetrics and Gynecology ,Gene Expression Regulation, Developmental ,ovogenèse ,microRNAs ,Ovaries ,Nucleic acids ,OVA ,micro arn ,Female ,Anatomy ,Cellular Types ,Genital Anatomy ,Research Article ,expression des gènes ,lcsh:QH426-470 ,qualité des oeufs ,Urology ,fecundity ,oryzia latipes ,reproduction ,Genetics ,Animals ,adrianichthyidae ,analyse du transcriptome ,Non-coding RNA ,Gonads ,CRISPR/Cas9 ,knock out ,fish ,medaka ,Granulosa Cells ,Gene Expression Profiling ,ovaire ,Reproductive System ,Biology and Life Sciences ,Subfertility ,Epithelial Cells ,Cell Biology ,Female Subfertility ,mécanisme de régulation ,Gene regulation ,lcsh:Genetics ,Germ Cells ,Biological Tissue ,Fertility ,Infertility ,fécondite ,Oocytes ,croissance folliculaire ,RNA ,Women's Health ,Lutein Cells ,ovary ,Gene expression ,CRISPR-Cas Systems - Abstract
Female gamete production relies on coordinated molecular and cellular processes that occur in the ovary throughout oogenesis. In fish, as in other vertebrates, these processes have been extensively studied both in terms of endocrine/paracrine regulation and protein expression and activity. The role of small non-coding RNAs in the regulation of animal reproduction remains however largely unknown and poorly investigated, despite a growing interest for the importance of miRNAs in a wide variety of biological processes. Here, we analyzed the role of miR-202, a miRNA predominantly expressed in male and female gonads in several vertebrate species. We studied its expression in the medaka ovary and generated a mutant line (using CRISPR/Cas9 genome editing) to determine its importance for reproductive success with special interest for egg production. Our results show that miR-202-5p is the most abundant mature form of the miRNA and that it is expressed in granulosa cells and in the unfertilized egg. The knock out (KO) of mir-202 gene resulted in a strong phenotype both in terms of number and quality of eggs produced. Mutant females exhibited either no egg production or produced a dramatically reduced number of eggs that could not be fertilized, ultimately leading to no reproductive success. We quantified the size distribution of the oocytes in the ovary of KO females and performed a large-scale transcriptomic analysis approach to identified dysregulated molecular pathways. Together, cellular and molecular analyses indicate that the lack of miR-202 impairs the early steps of oogenesis/folliculogenesis and decreases the number of large (i.e. vitellogenic) follicles, ultimately leading to dramatically reduced female fecundity. This study sheds new light on the regulatory mechanisms that control the early steps of follicular development, including possible targets of miR-202-5p, and provides the first in vivo functional evidence that a gonad-predominant microRNA may have a major role in female reproduction., Author summary The role of small non-coding RNAs in the regulation of animal reproduction remains poorly investigated, despite a growing interest for the importance of miRNAs in a wide variety of biological processes. Here, we analyzed the role of miR-202, a miRNA predominantly expressed in gonads in vertebrate. We studied its expression in the medaka ovary and knocked out the mir-202 gene to study its importance for reproductive success. We showed that the lack of miR-202 results in the sterility of both females and males. In particular, it led to a drastic reduction of both the number and the quality of eggs produced by females. Mutant females exhibited either no egg production or produced a drastically reduced number of eggs that could not be fertilized, ultimately leading to no reproductive success. Quantitative histological and molecular analyses indicated that mir-202 KO impairs oocyte development and is also associated with the dysregulation of many genes that are critical for reproduction. This study sheds new light on the regulatory mechanisms that control oogenesis, including possible targets of miR-202-5p, and provides the first in vivo functional evidence that a gonad-predominant microRNA may have a major role in female reproduction.
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- 2018
- Full Text
- View/download PDF
6. MiR-202 regulates female fecundity by controlling early follicle development in medaka ovary
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Gay, Stéphanie, Bugeon, Jérôme, Bouchareb, Amine, Henry, Laure, DELAHAYE, Clara, Montfort, Jérôme, Le Cam, Aurélie, Bobe, Julien, Thermes, Violette, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Instituto Nacional de Pesquisas da Amazônia (INPA). Manaus, BRA., and Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)
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fish ,medaka ,qualité des oeufs ,fecundity ,[SDV]Life Sciences [q-bio] ,fungi ,education ,analyse d'image ,ovogenèse ,humanities ,oryzias latipes ,reproduction ,oeuf de poisson ,poisson ,fécondite ,adrianichthyidae ,fluorescence ,analyse du transcriptome ,CRISPR/Cas9 ,health care economics and organizations ,reproductive and urinary physiology ,miRNA ,expression des gènes - Abstract
MiR-202 regulates female fecundity by controlling early follicle development in medaka ovary. 11. International Symposium on Reproductive Physiology of Fish
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- 2018
7. A transcriptomic comparison suggests an influence of the domestication process on egg quality in Eurasian Perch (Perca fluviatilis)
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Rocha de Almeida, Taina, Alix, M., Schaerlinger, B., Le Cam, Aurélie, Montfort, Jérôme, Toomey, L., Bobe, Julien, Chardard, D., Fontaine, Pascal, Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), ANR-13-BSV7-0015 - CNPq (233389/2014-8) - MESR, CNPq (233389/2014-8) - MESR, Instituto Nacional de Pesquisas da Amazônia (INPA). Manaus, BRA., ANR-13-BSV7-0015,Maternal Legacy,Portait moléculaire d'un oeuf de poisson de bonne qualité(2013), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Unité de Recherches Animal et Fonctionnalités des Produits Animaux ( URAFPA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Lorraine ( UL ), Laboratoire de Physiologie et Génomique des Poissons ( LPGP ), and Institut National de la Recherche Agronomique ( INRA ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
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fish ,perca fluviatilis ,[ SDV ] Life Sciences [q-bio] ,qualité des oeufs ,[SDV]Life Sciences [q-bio] ,education ,physiologie de la reproduction ,humanities ,reproduction ,perch ,oeuf de poisson ,domestication ,poisson ,perche ,percidae ,arn maternel ,health care economics and organizations ,plant domestication - Abstract
A transcriptomic comparison suggests an influence of the domestication process on egg quality in Eurasian Perch (Perca fluviatilis). 11. International Symposium on Reproductive Physiology of Fish
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- 2018
8. miR-202, un microARN clé pour le succès reproducteur des femelles chez le poisson médaka
- Author
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Gay, Stéphanie, Bugeon, Jérôme, Bouchareb, Amine, Henry, Laure, Montfort, Jérôme, Le Cam, Aurélie, Bobe, Julien, Thermes, Violette, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ProdInra, Archive Ouverte
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fish ,medaka ,breeding success ,qualité des oeufs ,[SDV]Life Sciences [q-bio] ,ovaire ,ovogenèse ,microRNAs ,oryzias latipes ,[SDV] Life Sciences [q-bio] ,reproduction ,oeuf de poisson ,poisson ,femelle ,micro arn ,ovary ,adrianichthyidae ,succes reproducteur ,CRISPR/Cas9 ,expression des gènes - Abstract
miR-202, un microARN clé pour le succès reproducteur des femelles chez le poisson médaka. Journées d'Animation Scientifique du département Phase (JAS Phase 2018)
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- 2018
9. MicroRNA-202 (miR-202) controls female fecundity by regulating medaka oogenesis
- Author
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Gay, Stéphanie, Bugeon, Jérôme, Bouchareb, Amine, Henry, Laure, Montfort, Jérôme, Le Cam, Aurélie, Bobe, Julien, and Thermes, Violette
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medaka ,fish ,fertility ,ovary ,gonads ,microRNAs ,quantitative image analyses ,CRISPR/cas9 ,microarray ,ovaire ,analyse d'image ,mécanisme de régulation ,oryzias latipes ,gonade ,reproduction ,fertilité ,reproduction femelle ,poisson ,micro arn ,adrianichthyidae ,analyse du transcriptome ,expression des gènes - Abstract
Female gamete production relies on coordinated molecular and cellular processes that occur in the ovary throughout oogenesis. In fish, as in other vertebrates, these processes have been extensively studied both in terms of endocrine/paracrine regulation and protein expression and activity. The role of small non-coding RNAs in the regulation of animal reproduction remains however largely unknown and poorly investigated, despite a growing interest for the importance of miRNAs in a wide variety of biological processes. Here, we analyzed the role of miR-202, a miRNA predominantly expressed in male and female gonads in several vertebrate species. We studied its expression in the medaka ovary and generated a mutant line (using CRISPR/Cas9 genome engineering) to determine its importance for reproductive success with special interest for egg production. Our results show that miR-202-5p is the biologically active form of the miRNA and that it is expressed in granulosa cells and in the unfertilized egg. The knock out (KO) of miR-202 resulted in a strong phenotype both in terms of number and quality of eggs produced. Mutant females exhibited either no egg production or produced a drastically reduced number of eggs that could not be fertilized, ultimately leading to no reproductive success. We quantified the size distribution of the oocytes in the ovary of KO females and performed a genome-wide transcriptomic analysis approach to identified dysregulated molecular pathways. Together, cellular and molecular analyses indicate that lack of miR-202 impairs the early steps of oogenesis/folliculogenesis and decreases the number of large (i.e. vitellogenic) follicles, ultimately leading to dramatically reduced female fecundity. This study sheds new light on the regulatory mechanisms that control the early steps of follicular development and provides the first in vivo functional evidence that an ovarian-predominant microRNA may have a major role in female reproduction.
- Published
- 2018
10. Expression of miRNA and target genes was affected by genetic selection of rainbow trout and plant-based diet
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ZHU, Tengfei, Corraze, Geneviève, Plagnes- Juan, Elisabeth, Montfort, Jérôme, Bobe, Julien, Quillet, Edwige, Dupont-Nivet, Mathilde, Skiba, Sandrine, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), and European Aquaculture Society (EAS). BEL.
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fish ,endocrine system ,disease resistance ,animal structures ,microRNA ,genetic selection ,plant-based diet ,urogenital system ,animal diseases ,[SDV]Life Sciences [q-bio] ,rainbow trout ,digestive system - Abstract
Expression of miRNA and target genes was affected by genetic selection of rainbow trout and plant-based diet . Aquaculture Europe 2017
- Published
- 2017
11. Transcriptomic analysis of Eurasian perch (Perca fluviatilis) eggs assigned to distinct groups of quality
- Author
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ROCHA DE ALMEIDA, Taina, Schaerlinger, Bérénice, Le Cam, Aurélie, Alix, Maud, Montfort, Jérôme, Bobe, Julien, CHARDARD, Dominique, Fontaine, Pascal, ProdInra, Archive Ouverte, Blanc 2013 - Portait moléculaire d'un oeuf de poisson de bonne qualité - - Maternal Legacy2013 - ANR-13-BSV7-0015 - Blanc 2013 - VALID, Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This study is funded in part by ANR (Agence Nationale de la Recherche/ French National Research Agency) Maternal Legacy (ANR-13-BSV7-0015). We are also thankful for INRA PHASE department for supporting this work and for the Brazilian National Council for Scientific and Technological Development (CNPq) and the French ministry of research for TRA and MA PhD fellowships, respectively., University of South Bohemia in České Budějovice. CZE., and ANR-13-BSV7-0015,Maternal Legacy,Portait moléculaire d'un oeuf de poisson de bonne qualité(2013)
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developmental success ,perca fluviatilis ,qualité des oeufs ,[SDV]Life Sciences [q-bio] ,education ,bacterialresistance transfer factor ,genomic ,reproduction ,fertilité ,perch ,oeuf de poisson ,analyse de génome ,poisson ,développement de l'ovocyte ,perche ,egg content ,percidae ,analyse du transcriptome ,oocyte ,fish ,ovocyte ,ovogenèse ,mortality ,sex factors ,[SDV] Life Sciences [q-bio] ,microarray ,mortalité ,expression des gènes - Abstract
Transcriptomic analysis of Eurasian perch (Perca fluviatilis) eggs assigned to distinct groups of quality. 6. International Workshop on the Biology of Fish Gametes
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- 2017
12. Characterization of rainbow trout gonad, brain and gill deep cDNA repertoires using a Roche 454-Titanium sequencing approach
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Le Cam, Aurélie, Bobe, Julien, Bouchez, Olivier, Cabau, Cédric, Kah, Olivier, Klopp, Christophe, Lareyre, Jean-Jacques, Le Guen, Isabelle, Lluch, Jérôme, Montfort, Jérôme, Moreews, Francois, Nicol, Barbara, Prunet, Patrick, Rescan, Pierre-Yves, Servili, Arianna, and Guiguen, Yann
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RAINBOW trout , *ANTISENSE DNA , *ADRENOCORTICOTROPIC hormone , *ADENOSINE monophosphate , *RYANODINE receptors , *ZONA pellucida , *TRANSCRIPTION factors , *NUCLEOTIDE sequence - Abstract
Abstract: Rainbow trout, Oncorhynchus mykiss, is an important aquaculture species worldwide and, in addition to being of commercial interest, it is also a research model organism of considerable scientific importance. Because of the lack of a whole genome sequence in that species, transcriptomic analyses of this species have often been hindered. Using next-generation sequencing (NGS) technologies, we sought to fill these informational gaps. Here, using Roche 454-Titanium technology, we provide new tissue-specific cDNA repertoires from several rainbow trout tissues. Non-normalized cDNA libraries were constructed from testis, ovary, brain and gill rainbow trout tissue samples, and these different libraries were sequenced in 10 separate half-runs of 454-Titanium. Overall, we produced a total of 3million quality sequences with an average size of 328bp, representing more than 1Gb of expressed sequence information. These sequences have been combined with all publicly available rainbow trout sequences, resulting in a total of 242,187 clusters of putative transcript groups and 22,373 singletons. To identify the predominantly expressed genes in different tissues of interest, we developed a Digital Differential Display (DDD) approach. This approach allowed us to characterize the genes that are predominantly expressed within each tissue of interest. Of these genes, some were already known to be tissue-specific, thereby validating our approach. Many others, however, were novel candidates, demonstrating the usefulness of our strategy and of such tissue-specific resources. This new sequence information, acquired using NGS 454-Titanium technology, deeply enriched our current knowledge of the expressed genes in rainbow trout through the identification of an increased number of tissue-specific sequences. This identification allowed a precise cDNA tissue repertoire to be characterized in several important rainbow trout tissues. The rainbow trout contig browser can be accessed at the following publicly available web site (http://www.sigenae.org/). [Copyright &y& Elsevier]
- Published
- 2012
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