1. Characterization of the mechanism underlying stonustoxin-mediated relaxant response in the rat aorta in vitro.
- Author
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Sung JM, Low KS, and Khoo HE
- Subjects
- Animals, Aorta physiopathology, Drug Interactions, Enzyme Inhibitors pharmacology, Male, Neurokinin-1 Receptor Antagonists, Nitric Oxide Synthase antagonists & inhibitors, Nitric Oxide Synthase Type II, Potassium Channel Blockers pharmacology, Rats, Rats, Sprague-Dawley, Thiazines pharmacology, Tryptophan analogs & derivatives, Tryptophan pharmacology, Aorta drug effects, Fish Venoms pharmacology, Substance P metabolism, Vasodilation drug effects
- Abstract
Stonustoxin (SNTX) is a lethal factor isolated from the venom of the stonefish Synanceja horrida. Although SNTX exhibits a multitude of biological activities, the primary cause of death upon administration of the toxin is attributed to marked hypotension. We investigated the possible mechanisms underlying the vascular hyporeactivity of this novel toxin. Cumulative doses of SNTX (5-320 ng/mL) induced concentration-dependent relaxation in phenylephrine (PE)--precontracted rat aortic rings with intact endothelium. Endothelium removal abolished the relaxation induced by SNTX. Tetraethylammonium (TEA), an inhibitor of K(+) channels, partially inhibited SNTX-induced relaxation. Similarly, SNTX-induced relaxation was partially attenuated by the SP receptor antagonist (NATB), whereas the inducible iNOS inhibitor, AMT-HCl, completely abolished the relaxation caused by SNTX. From the results obtained, it can be postulated that a component of SNTX-mediated vasorelaxation is via binding of either SNTX or SP to the SP receptors that are located on the endothelial cells. Occupation of these SP receptors causes subsequent production of NO and activation of K(+) channels, thus leading to vasorelaxation of the rat aortic rings.
- Published
- 2002
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