Stable carbon and nitrogen isotope signatures have been determined for littoral fishes from three localities in the Balearic Islands (NW Mediterranean) for trophic assessment. Variation in the isotopic composition was examined at multiple levels: the individual organism, individual populations (ontogenetic variation) and at area effects (different islands). Cluster analyses based on d13C and d15N resulted in two very different dendrograms for the fish assemblages from Menorca and Formentera. In both schemes, congeners of the generaDiplodusandLabrusdid not cluster together, suggesting that closely related species do not necessarily feed on similar foods. Significant differences in d15N were detected between locations forScorpaena porcus, Scorpaena scrofaandSciaena umbra,and significant differences in d13C were detected forScorpaena porcusandScorpaena scrofa. Overall there were significant spatial differences in the trophic position of littoral fishes among the three Balearic Islands, and fishes from Palma, Mallorca tended to feed at a higher trophic level than fishes from either Menorca and Formentera. Large-scale geographic comparisons, using earlier data from three sites in western Mallorca and in Corsica revealed that throughout the western Mediterranean generally, the average trophic level of fishes differs remarkably little. However, large discrepancies were observed in d15N for individual species, notablyScorpaena porcus,D. annularis, D. vulgarisandSerranus scriba.13C composition of fishes did not seem to change greatly among the locations, although marked discrepancies were observed forD. puntazzoandMullus surmuletus. Given the large-scale patterns we have observed, it is possible that the extent of multichannel omnivory which has been purported to exist in complex aquatic systems, may have been greatly overstated. Regression of d15N and d13C with fish body size revealed clear and significant trends for some species. In particular, d15N appeared to increase with body length in the macrocarnivorous fishesScorpaena porcus, Scorpaena scrofa,Serranus scribaandSciaena umbra. Trends were less apparent in animals which feed primarily on small benthic invertebrates, notably the fourDiplodusspecies (D. vulgaris, D. sargus, D. annularis, D. puntazzo), and the labridsLabrus viridisandL. merula. [ABSTRACT FROM AUTHOR]