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Your search keyword '"Mosquito Flavivirus"' showing total 4 results
Start Over Descriptor "Mosquito Flavivirus" Topic flavivirus
4 results on '"Mosquito Flavivirus"'

1. In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides .

Author

Huang Y, Zhang H, Li X, Zhao L, Cai D, Wang S, Ren N, Ma H, Huang D, Wang F, Yuan Z, Zhang B, and Xia H

Subjects
Animals, China, Phylogeny, RNA, Aedes, Ceratopogonidae, Culicidae, Flavivirus, Insect Viruses genetics
Abstract

Mosquito-specific flaviviruses comprise a group of insect-specific viruses with a single positive RNA, which can affect the duplication of mosquito-borne viruses and the life growth of mosquitoes, and which have the potential to be developed as a vaccine platform for mosquito-borne viruses. In this study, a strain of mosquito flavivirus (MFV) YN15-283-02 was detected in Culicoides collected from Yunnan, China. The isolation of the purified MFV YN15-283-02 from cell culture failed, and the virus was then rescued by an infectious clone. To study the biological features of MFV YN15-283-02 in vitro and in vivo, electron microscopy, phylogenetic tree, and viral growth kinetic analyses were performed in both cell lines and mosquitoes. The rescued MFV (rMFV) YN15-283-02 duplicated and reached a peak in C6/36 cells at 6 d.p.i. with approximately 2 × 10 6 RNA copies/μL (RNA to cell ratio of 0.1), but without displaying a cytopathic effect. In addition, the infection rate for the rMFV in Ae. aegypti show a low level in both larvae (≤15%) and adult mosquitoes (≤12%).

Published
2022
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2. Screening for Viruses and Lemur-Associated Filara in Wild-Caught Mosquitoes From Madagascar.

Author

Klein A, Strube C, Becker SC, and Naccache F

Subjects
Aedes parasitology, Aedes virology, Animals, Anopheles parasitology, Anopheles virology, Biodiversity, Culex parasitology, Culex virology, Disease Reservoirs, Filariasis transmission, Lemur, Madagascar, Virus Diseases transmission, Flavivirus isolation & purification, Mosquito Vectors parasitology, Mosquito Vectors virology, Nematoda isolation & purification
Abstract

Madagascar is a hotspot of biodiversity, but poverty and population growth provoke a high risk of conflict between food security and biodiversity conservation in this tropical country. Numerous vector-borne diseases, including viral infections, affect public health in Madagascar and a continuous expansion of anthropogenically used areas intensifies contact on the human-wildlife interface. However, data on human and animal pathogens in potential insect vectors is limited. Therefore, we conducted a parasitological and virological survey of 785 adult female mosquitoes between March and May 2016 at the Ankarafantsika National Park in northwestern Madagascar. Screening included Alpha-, Phlebo-, and Flaviviridae and the recently described filarial nematode species, Lemurfilaria lemuris. The predominant mosquito genus was Culex (91%), followed by Mansonia (4.1%), Anopheles (3.4%), and Aedes (0.9%). Viral screening revealed no arboviruses, but an insect-specific flavivirus in two Culex sitiens pools. No pools screened positive for the lemur-specific filarial nematode L. lemuris., (© The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Published
2021
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3. Field-captured Aedes vexans (Meigen, 1830) is a competent vector for Rift Valley fever phlebovirus in Europe

Author

Ana I. Núñez, Carles Aranda, Núria Busquets, Sandra Talavera, Sebastian Napp, Lotty Birnberg, Producció Animal, and Sanitat Animal

Subjects
0301 basic medicine, Phlebovirus, Rift Valley Fever, Rain, Aedes vexans, 0302 clinical medicine, Aedes, Zoonoses, Chlorocebus aethiops, Rift Valley fever, education.field_of_study, biology, Zoonosis, Ruminants, Specific Pathogen-Free Organisms, Europe, Flavivirus, Infectious Diseases, Mosquito Flavivirus, Female, 030231 tropical medicine, Population, Alphavirus, Mosquito Vectors, lcsh:Infectious and parasitic diseases, Cell Line, 03 medical and health sciences, medicine, Animals, Humans, lcsh:RC109-216, education, Saliva, Vero Cells, Research, RVFV, Water, medicine.disease, biology.organism_classification, Rift Valley fever virus, Virology, Floods, Clone Cells, 030104 developmental biology, Parasitology, Spain, Cattle, Vector competence, Chickens
Abstract

BackgroundAedes vexans(Meigen) is considered a nuisance species in central Europe and the Mediterranean region. It is an anthropophilic and mammalophilic floodwater mosquito involved in the transmission of several arboviruses. Rift Valley fever (RVF) is a relevant mosquito-borne zoonosis, affecting mainly humans and ruminants, that causes severe impact in public health and economic loses. Due to globalization and climate change, the European continent is threatened by its introduction. The main purpose of the present study was to evaluate the vector competence of a European field-collectedAe. vexanspopulation.MethodsAedes vexansfield-collected larvae were reared in the laboratory under field-simulated conditions. To assess the vector competence for Rift Valley fever phlebovirus (RVFV) transmission, adult F0 females were exposed to infectious blood meals containing the 56/74 RVFV strain. Additionally, intrathoracic inoculations with the same virus strain were performed to evaluate the relevance of the salivary gland barriers. Natural circulation of alphavirus, flavivirus and phlebovirus was also tested.ResultsTo our knowledge, an autochthonousAe. vexanspopulation was experimentally confirmed as a competent vector for RVFV for the first time. This virus was capable of infecting and disseminating within the studiedAe. vexansmosquitoes. Moreover, infectious virus was isolated from the saliva of disseminated specimens, showing their capacity to transmit the virus. Additionally, a natural infection with a circulating Mosquito flavivirus was detected. The co-infection with the Mosquito flavivirus seemed to modulate RVFV infection susceptibility in field-collectedAe. vexans, but further studies are needed to confirm its potential interference in RVFV transmission.ConclusionsOur results show that field-collected EuropeanAe. vexanswould be able to transmit RVFV in case of introduction into the continent. This should be taken into consideration in the design of surveillance and control programmes.

Published
2019

4. Isolation and genomic characterization of Chaoyang virus strain ROK144 from Aedes vexans nipponii from the Republic of Korea

Author

Terry A. Klein, John S. Lee, Nathan D. Grubaugh, Monica L. O'Guinn, Michael J. Turell, John P. Kondig, and Heung-Chul Kim

Subjects
Sequence analysis, viruses, Arbovirus, Genome, Polymerase Chain Reaction, law.invention, Cell Line, law, Aedes, Virology, parasitic diseases, Republic of Korea, medicine, Animals, Arthropod-specific flavivirus, Vero Cells, Polymerase chain reaction, Cells, Cultured, Phylogeny, Aedes vexans, biology, Base Sequence, Strain (biology), Flavivirus, Mosquito flavivirus, Genomics, Sequence Analysis, DNA, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Isolation (microbiology), medicine.disease, Insect Vectors, Vector surveillance, RNA, Viral
Abstract

During June 2003, mosquito surveillance was conducted at a US Army installation and a US Military training site 2km south of the demilitarized zone, Republic of Korea. Mosquitoes were collected using Mosquito Magnets™, sorted to species, and assayed for the presence of arboviruses. From the 3,149 mosquitoes that were sorted into 126 pools, one Aedes vexans nipponii pool (out of 73 pools) tested positive for flavivirus RNA by reverse transcription-PCR. After isolation from C6/36 cell culture supernatant, the viral genome was sequenced and found to be 98.9% related to Chaoyang virus, a potential arthropod-specific flavivirus. This report details the first identification of Chaoyang virus in the Republic of Korea and highlights its relationship to other flaviviruses.

Published
2012

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