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This study aimed to identify the chemical composition of the Spondias tuberosa aqueous leaf and root extracts (EALST and EARST) and to evaluate their effect, comparatively, against opportunistic pathogenic fungi. Ultra-Performance Liquid Chromatography Coupled to a Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF) was employed for chemical analysis. Candida albicans and C. tropicalis standard strains and clinical isolates were used (CA INCQS 40006, CT INCQS 40042, CA URM 5974, and CT URM 4262). The 50% Inhibitory Concentration for the fungal population (IC50) was determined for both the intrinsic action of the extracts and the extract/fluconazole (FCZ) associations. The determination of the Minimum Fungicidal Concentration (MFC) and the verification of effects over fungal morphological transitions were performed by subculture in Petri dishes and humid chambers, respectively, both based on micro-dilution. UPLC-MS-ESI-QTOF analysis revealed the presence of phenolic and flavonoid compounds. The association of the extracts with fluconazole, resulted in IC50 values from 2.62 µg/mL to 308.96 µg/mL. The MFC of the extracts was ≥16,384 µg/mL for all tested strains, while fluconazole obtained an MFC of 8192 µg/mL against C. albicans strains. A reduction in MFC against CA URM 5974 (EALST: 2048 µg/mL and EARST: 1024 µg/mL) occurred in the extract/fluconazole association. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the behavior of slow and fast-darkening carioca beans during storage, and the profile of phenolics and saponins, aiming to identify which compounds may be involved in the browning process of the integument of these grains. Cultivars Madreperola and Dama presented the lowest total color difference (ΔE) and the highest luminosity (L*) values during storage time indicating to be slow-darkening cultivars. In contrast, the cultivars Notavel and Imperador rapidly darkened with storage time, evidenced by the highest ΔE and lowest L*. Regarding the content of phenolic during storage, it was observed a reduction for free phenolics in fast-darkening beans and the maintenance/increase in slow-darkening beans. In the PCA of the phenolic profile, Notavel and Imperador were similar, differing from Dama and Madreperola, indicating that fast-darkening grains have similar phenolic profile. The OPLS/DA indicated that aged grains from bright beans had more kaempferol than darker beans. For saponins, the cultivars Imperador and Notavel presented higher content of Soyasaponin Bd, which might be related to the darkening process. Besides, kaempferol was found at higher concentrations in slow-darkening aged grains and can be a chemical marker of darkening in carioca beans. • The content of phenolic compounds reduces in fast darkening beans during storage. • Kaempferol can be a marker to differ carioca bean with fast darkening profile. • Beans with higher content of Soyasaponin Bd darken fast. [ABSTRACT FROM AUTHOR]

Cordiera myrciifolia is an abundant species in Northeast Brazil that presents metabolites of biological/therapeutic interest. From this perspective, the present study aimed to investigate the chemical constituents and evaluate the in vitro antimicrobial activity of hexane (HECM) and ethanolic (EECM) extracts of C. myrciifolia leaves. The extracts were analyzed by chromatographic techniques (GC and UPLC) coupled with mass spectrometry. The antimicrobial activity of the extracts and the extracts combined with conventional drugs was evaluated by microdilution. The in vitro effect of the treatments on Candida 's morphological transition was verified through cultivation in humid chambers. In HECM, 11 constituents including fatty acids, and triterpenes, including phytosterols, alkanes, tocols, and primary alcohols were identified. Triterpenes represented >40% of the identified constituents, with Lupeol being the most representative. In EECM, 13 constituents were identified, of which eight belonged to the class of flavonoids. High antibacterial activity of HECM was detected against Escherichia coli and Staphylococcus aureus , with Minimum Inhibitory Concentrations of 8 and 16 μg/mL, respectively. The combined activity was more effective when combined with Norfloxacin and Imipenem. In anti- Candida activity, the IC 50 of the extracts ranged from 36.6 to 129.1 μg/mL. There was potentiating effect when associated with Fluconazole. Both extracts inhibited the filamentous growth of C. tropicalis at a concentration of 512 μg/mL. C. myrciifolia extracts prove to be candidates for the development of new therapeutic formulations to treat bacterial and fungal infections. [Display omitted] • The hexane extract of Cordiera myrciifolia (HECM) has antibacterial effect. • The ethanolic extract (EECM) potentiates the action of Norfloxacin and Imipenem. • HECM and EECM showed antifungal activity against Candida. • HECM shows lupeol as major compound and EECM contains a large number of flavonoids. [ABSTRACT FROM AUTHOR]