1. Rapid screening and identification of compounds with DNA-binding activity from Folium Citri Reticulatae using on-line HPLC-DAD-MS(n) coupled with a post column fluorescence detection system.
- Author
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Fu Q, Zhang C, Lin Z, Sun H, Liang Y, Jiang H, Song Z, Wang H, and Chen S
- Subjects
- Acids, Carbocyclic analysis, Acids, Carbocyclic chemistry, Flavonoids chemistry, Hesperidin analysis, Linear Models, Plant Extracts chemistry, Plant Leaves chemistry, Quercetin analysis, Reference Standards, Reproducibility of Results, Chromatography, High Pressure Liquid methods, Citrus chemistry, DNA chemistry, Flavonoids analysis, Fluorescence, Mass Spectrometry methods
- Abstract
To study the interactions between natural compounds and deoxyribonucleic acid (DNA), a method has been established combining a high-performance liquid chromatography-diode array detector-multi-stage mass spectrometer with a fluorescence detector (HPLC-DAD-MS(n)-FLD). The FLD was used to monitor fluorescence intensity of the ethidium bromide-DNA (EB-DNA) complex when a compound separated by HPLC was introduced. This novel method was used to simultaneously obtain the HPLC fingerprint, UV spectra, MS(n) fragments and DNA-binding activity profile of various components in Folium Citri Reticulatae. As a result, 35 compounds were identified, of which 25 were found in the extract of Folium Citri Reticulatae for the first time, and 33 compounds showed DNA-binding activities, with the most active being feruloylhexaric and p-coumaroylhexaric acids. In addition, the precision, stability and reproducibility of this method were validated by two positive controls, quercetin and hesperidin. This new on-line method is accurate, precise and reliable for further high-throughput screening of DNA-binding compounds from food samples and other complex matrices., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2016
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