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Abstract It is not straightforward to link biofilm parameters to frictional drag, because of the heterogeneous distribution and viscoelasticity of the produced matrix. Here we present the design and calibration of a flow cell in which marine biofilms can be cultured under flow and then assessed for drag, structural and mechanical properties. The flow cell test section comprised a rectangular channel constructed by sandwiching together rigid PVC panels and side panels of clear acrylic, which allow natural light to enter. The Fanning friction factor (C f) of the flow cell was found by measuring the pressure drop (ΔP) at various flow velocities (u). Flow cell calibration was carried out using a clean inert marine coating, and various roughness grades of sandpaper sheets to find C f for each rigid roughness. ΔP was proportional to u 2, indicating flow was turbulent (R2 = 0.99). The top panel of the flow cell can be substituted with a clear acrylic lid to allow simultaneous measurement of C f and biofilm physico-mechanical properties by optical coherence tomography (OCT). Here, we demonstrated that the flow cell can be used to image microbial fouling using OCT. Future experiments will assess physico-mechanical and drag properties of marine fouling biofilms under flow. [ABSTRACT FROM AUTHOR]

Abstract A novel fouling marine flow cell was designed and fitted with a clear plastic lid to allow real-time imaging of biofilms using optical coherence tomography (OCT). Marine biofilms were grown under controlled shear flow on coupons coated with 6 different biocidal antifouling coatings (SPC1, SPC2, SPC3, SPC4, CDP1 and CDP2, AkzoNobel) and one inert coating which contained no biocidal actives (NB-D) for 8 weeks. One set of coupons coated with NB was statically immersed in sea water during the same time period (NB-S). Biofilm removal was assessed by increasing the flow velocity while OCT simultaneously measured the biofilm cross-sectional area (CSA). The highest initial removal rates were observed for NB-S, NB-D, and SPC2. Percent biofilm cross-sectional area reduction (%CSAred) was higher on SPCs (>60%) compared to CDPs (<50%). SPCs had the highest percent reduction in biofilm surface area coverage (%SACred >60%) compared to the CDPs (<20%). The marine biofilm flow cell combined with OCT can be used to screen for coating-specific differences in biofilm growth and removal in real time rather than traditional before and after surface area coverage measurements. Future testing will focus on how the biofilm-coatings interactions interact with biofilm mechanical and structural properties to produce drag. [ABSTRACT FROM AUTHOR]

Biofilm heterogeneity and adaptability complicates efforts to link biofilm structural and mechanical properties to frictional drag. As a result, rigid structures are typically used as the benchmark for studying biofilm-associated drag. Elastomeric sandpaper replicas were generated to be used as model systems for investigating the effect of roughness and elasticity on drag, over the Reynolds number range of approximately 2.0 × 104 to 5.2 × 104 Re using a marine biofilm flow cell. To control for roughness parameters and surface topography the replicas were created for sandpaper grit numbers: P40, P80 and P240 with average measured roughness (S a) of 108, 49 and 16 μm, respectively. Profilometry confirmed that there was no significant difference between the roughness of the rigid sandpaper sources and the material replicas. The marine biofilm flow cell was fitted with a clear lid, which allowed real-time visualisation of the replicas' surface topography using Optical Coherence Tomography. Pressure drop measurements, expressed as a friction coefficient, revealed that the elastomeric sandpaper replicas had a significantly higher associated drag, of up to 52%, when compared to the rigid counterparts. From statistical analysis it was confirmed that material mechanical properties, such as elasticity, and surface roughness both significantly affect drag. Elastic model systems can be used to enhance our understanding of biofilm physico-mechanics and their role in marine drag. • Created a novel method for creating an elastomeric biofilm model. • Derived mesoscopic structural parameters in-situ using OCT. • Roughness and elasticity had a significant shared and independent effect on drag. • Elastomeric replicas increased drag by up to 52% compared to rigid structures. • Importance of the effect of elasticity on drag is highlighted. [ABSTRACT FROM AUTHOR]