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Your search keyword '"Aasted, B."' showing total 2 results
Start Over Author "Aasted, B." Topic flow cytometry Topic monoclonal antibodies
2 results on '"Aasted, B."'

1. Analyses of monoclonal antibodies reacting with porcine wCD6 Results from the Second International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Bullido, R., Trebichavsky, I., Novikov, B., Valpotic, I., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Petrinec, N., and Saalmüller, A.

Subjects
Antigens, Differentiation, T-Lymphocyte, General Veterinary, Swine, T-cells, Immunology, Antibodies, Monoclonal, Flow Cytometry, Antigen-Antibody Reactions, Porcine wCD6, Antigens, CD, T-Lymphocyte Subsets, Monoclonal antibodies, porcine wCD6, Animals
Abstract

Among the 57 monoclonal antibodies analyzed within the T-cell group of the Second International Swine CD Workshop, one mAb fell within cluster T14a that included the CD6 standard a38b2 (No. 175). The new mAb MIL8 (No. 082) and a38b2 both precipitated from activated T-cells a 150 kDa monomeric protein. Staining patterns on the various cell types were similar. There was no inhibition of binding of either mAb to peripheral blood T-cells with the opposite mAb. The new mAb, MIL8, reacts with a separate epitope on porcine wCD6.

Published
1998

2. Identification of monoclonal antibodies that cross-react with cytokines from different animal species

Author

Pedersen, L.G., Castelruiz, Y., Jacobsen, S., and Aasted, B.

Subjects
*MONOCLONAL antibodies, *CYTOKINES, *FLOW cytometry
Abstract

Eleven monoclonal antibodies specific for ovine, bovine and human cytokines were investigated by flow cytometry for cross-reactivities with cytokines produced by peripheral blood mononuclear cells (PBMCs) from sheep, cattle, goat, swine, horse, dog, mink, rabbit and human. Four antibodies specific for IL-4, IL-8, IFN-γ and TNF-α cross-reacted with cytokines from a majority of the species investigated. These antibodies can be applied to flow cytometric studies of cytokine production by PBMCs from several veterinary species. Another five antibodies specific for IL-2, IL-6, GM-CSF and IFN-γ (two antibodies) cross-reacted weakly and with a variable number of animal species. These antibodies could in certain situations be useful in flow cytometry. In a number of cases the immunological cross-reactivities were confirmed by Western blot analyses. Overall, the results of this study will remedy some of the lack of species-specific anti-cytokine antibodies in veterinary research. [Copyright &y& Elsevier]

Published
2002
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