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Fluvial biological habitat types are classified using the diversity in physical characteristics of a water channel. Recent ecological management studies have highlighted the potential of underwater sound as a quantitative indicator of habitat characteristics. We investigate the relationship between underwater acoustic characteristics and hydraulic factors of 12 habitat types in the Namdae Stream in Yangyang, Korea, namely riffles, pools, and step riffle habitats. In the riffles and pools, the underwater sound levels were measured as sound pressure levels (SPLs). SPL(RMS) and 1/3 octave band have been measured in the frequency range between 8 Hz and 20 kHz. Among riffles, high SPL corresponded to the descending level of flow velocity. Pools generally had a low SPL. Low-frequency sound waves in the upper regions are better transmitted in the deeper water. To quantitatively analyze the water depth and flow velocity, we used a regression between the observed water depth, flow velocity, and acoustic SPL. The application of this study was certificated. The correlation coefficients between SPL and flow velocity/water depth revealed specific frequency bands with very strong positive correlations between SPL and flow rate in riffles and very strong negative correlations between SPL and pool water depth. Consequently, underwater sound can be used as an alternative for evaluating biological habitats. [ABSTRACT FROM AUTHOR]