Search

β-Galactosidase (β-gal), an enzyme related to cell wall degradation, plays an important role in regulating cell wall metabolism and reconstruction. However, activatable fluorescence probes for the detection and imaging of β-gal fluctuations in plants have been less exploited. Herein, we report an activatable fluorescent probe based on intramolecular charge transfer (ICT), benzothiazole coumarin-bearing β-galactoside (BC-βgal), to achieve distinct in situ imaging of β-gal in plant cells. It exhibits high sensitivity and selectivity to β-gal with a fast response (8 min). BC-βgal can be used to efficiently detect the alternations of intracellular β-gal levels in cabbage root cells with considerable imaging integrity and imaging contrast. Significantly, BC-βgal can assess β-gal activity in cabbage roots under heavy metal stress (Cd2+, Cu2+, and Pb2+), revealing that β-gal activity is negatively correlated with the severity of heavy metal stress. Our work thus facilitates the study of β-gal biological mechanisms. [Display omitted] • An activatable fluorescence probe (BC-βgal) for detecting β-galactosidase was designed. • BC-βgal was high sensitivity and selectivity towards β-gal with fast response (8 min). • BC-βgal imaged the β-gal fluctuation in situ in cabbage roots. • BC-βgal evaluated the β-gal activity under heavy metal stress for the first time. [ABSTRACT FROM AUTHOR]