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Start Over Author "Cui, Shangjin" Topic fluorescence Topic laboratory swine
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1. Establishment of real-time TaqMan-fluorescence quantitative RT-PCR assay for detection and quantitation of ten kinds of porcine inflammation markers mRNA.

Author

Lin, Wencheng, Wang, Zhongtian, Qiu, Zheng, Liu, Qinfang, and Cui, Shangjin

Subjects
*INFLAMMATORY mediators, *FLUORESCENCE, *POLYMERASE chain reaction, *MESSENGER RNA, *LABORATORY swine, *CYTOKINES
Abstract

Highlights: [•] Quantitative real-time PCR of 8 cytokines was established. [•] The established assays were highly specific and sensitive. [•] The transcriptive levels of mRNA of IFN-α, IFN-β, RIG-1 and IRF-7 were up-regulated. [•] The levels of TLR-9, IRF-3, JAK-1, JAK-2, STAT-1 and STAT-2 have barely changes. [ABSTRACT FROM AUTHOR]

Published
2013
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