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To realize sensing and labeling biomarkers is quite challenging in terms of designing multimodal imaging probes. In this study, we developed a novel β‐galactosidase (β‐gal) activated bimodal imaging probe that combines near‐infrared (NIR) fluorescence and magnetic resonance imaging (MRI) to enable real‐time visualization of activity in living organisms. Upon β‐gal activation, Gal‐Cy‐Gd‐1 exhibits a remarkable 42‐fold increase in NIR fluorescence intensity at 717 nm, allowing covalent labeling of adjacent target enzymes or proteins and avoiding molecular escape to promote probe accumulation at the tumor site. This fluorescence reaction enhances the longitudinal relaxivity by approximately 1.9 times, facilitating high‐resolution MRI. The unique features of Gal‐Cy‐Gd‐1 enable real‐time and precise visualization of β‐gal activity in live tumor cells and mice. The probe's utilization aids in identifying in situ ovarian tumors, offering valuable assistance in the precise removal of tumor tissue during surgical procedures in mice. The fusion of NIR fluorescence and MRI activation through self‐immobilizing target enzymes or proteins provides a robust approach for visualizing β‐gal activity. Moreover, this approach sets the groundwork for developing other activatable bimodal probes, allowing real‐time in vivo imaging of enzyme activity and localization. [ABSTRACT FROM AUTHOR]

Keywords: 19F MRI; Biosensors; Fluorescence Imaging; Hypoxia; Nitroreductase EN 19F MRI Biosensors Fluorescence Imaging Hypoxia Nitroreductase 1 1 1 12/07/22 20221212 NES 221212 B Eine bimodale Bildgebungssonde b FCy7-NO SB 2 sb wurde eingesetzt, um die bei Lungenkrebs überexprimierte Nitroreduktase in vivo abzubilden, wie Xin Zhou et al. in ihrem Forschungsartikel berichten (e202213495). 19F MRI, Biosensors, Fluorescence Imaging, Hypoxia, Nitroreductase Die Integration zweier komplementärer Bildgebungstechnologien in FCy7-NO SB 2 sb ermöglicht einen empfindlichen Nachweis von Nitroreduktase in einem breiten Konzentrationsbereich ohne Gewebetiefenbegrenzung. [Extracted from the article]