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[Display omitted] • Fluorescence-method for specific determination of natamycin was designed. • Determination of the studied drug in yoghurt samples. • Facile application of the designed fluorescence-method. • The developed method can present a fascinating methodology for natamycin analysis. This study aims to develop a novel and selective method for the detection of natamycin (E235) in yoghurt. The suggested method adopts an application of Hantzsch reaction to turn on the fluorescence behavior of natamycin (blue fluorescence), allowing its sensitive and selective determination in yoghurt samples without any overlapping at 485 nm. The originality of the research lies in the fact that this application takes place for the first time, also the detection (LOD) and quantification (LOQ) limits were very low (0.02 and 0.06 μ g mL−1, respectively) with a linear concentration range of 0.1–1.0 μ g m L - 1 . Moreover, the developed method was employed for the detection of E235 in yoghurt sample with a good recoveries (98.80 ± 1.20–99.20 ± 1.15 (%), over a concentration range of 0.5–1.0 μ g m L - 1 , (LOD = 0.04 and LOQ = 0.12 μ g m L - 1 ). Furthermore, the specificity and convenient application of our intended method is an attempt to determine E235 in milk and dairy products with easily followable steps. [ABSTRACT FROM AUTHOR]

In this work, the fluorescence of mirabegron was turned-on using one-pot Hantzsch 1,4 dihydropyridine synthesis, permitting its sensitive and selective determination in human plasma and pharmaceutical dosage form. [Display omitted] • A novel fluorescence-method for selective determination of mirabegron was designed. • The developed method can present a fascinating methodology for mirabegron analysis. • The sensing strategy displayed a good linear range and excellent application prospects. • Optimization of the experimental conditions through design of experiments. Here, a novel fluorescence strategy was established for the detection of mirabegron (MBG) sensitively on the basis of hantzsch dihydropyridine synthesis. The developed method adopts turn-on fluorescence of MBG for the first time, permitting its selective determination in spiked human plasma at 486 nm after excitation at 410 nm. The developed method exhibited a good linear range from 0.5 μ g m L - 1 to 2.0 μ g m L - 1 with detection and quantification limits of 0.05 and 0.2 (μ g m L - 1), respectively. The profitable applicability of the developed method in spiked human plasma samples was demonstrated, achieving limit of detection below the previously levels reported by spectroscopic methods, allowing application of the developed method for selective determination of MBG in its tablets and spiked human plasma samples with good recovery. [ABSTRACT FROM AUTHOR]