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Start Over Author "Nothisen M" Topic fluorescence resonance energy transfer
1 results on '"Nothisen M"'

1. Bio-specific and bio-orthogonal chemistries to switch-off the quencher of a FRET-based fluorescent probe: application to living-cell biothiol imaging.

Author

Egloff C, Jacques SA, Nothisen M, Weltin D, Calligaro C, Mosser M, Remy JS, and Wagner A

Subjects
3T3 Cells, Animals, Mice, Fluorescence Resonance Energy Transfer methods, Fluorescent Dyes chemistry, Molecular Imaging methods, Sulfhydryl Compounds analysis
Abstract

We report the first molecular system that is responsive to both a bio-specific and a bio-orthogonal stimulus. This dual activation process was applied to the design of a biothiol-specific FRET-based fluorescent probe that could be turned-on via an original concept of quencher bleaching.

Published
2014
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