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Reported here is a novel ratiometric and colorimetric fluorescent probe 1 for hypochlorite based on cyanobiphenyl and diaminomaleonitrile. This probe 1 was designed based on the mechanism that ClO− selectively cleaved the hydrazone bond (-C=N-) in this probe and released the fluorophore, 3'-formyl-4'-hydroxy-4-biphenylcarbonitrile. The addition of ClO− to the solution of probe 1 resulted in a very large blue-shift in both fluorescence (107 nm) spectra and an obvious fluorescence color change from red to green. Furthermore, this probe displays a rapid response (30 s) and a low detection limit (3.34 × 10−7 M, based on LOD = 3σ/slope) in detecting ClO−. Importantly, practical utility of this probe for the selective detection of ClO− in living cells has been successfully demonstrated, illustrating the great potential for biological analysis. Additionally, the probe 1 was also successfully applied to the detection of ClO− in real water sample. Unlabelled Image • A ratiometric and colorimetric fluorescent probe for ClO− was designed and synthesized. • The addition of ClO− resulted in a very large blue-shift in emission spectra and an obvious fluorescence color change. • This probe displays a rapid response and a low detection limit in detecting ClO−. • This probe for the selective detection of ClO− in living cells has been successfully demonstrated. • The probe 1 was also successfully applied to the detection of ClO− in real water sample. [ABSTRACT FROM AUTHOR]

A fluorescence probe (HBM) based on conjugation of 3′-formyl-4′-hydroxy-4-biphenylcarbonitrile to 2,4-dinitrophenylhydrazune for the detection of hypochlorite has been developed. The probe exhibited a highly sensitive and selective response toward hypochlorite (ClO − ) with a low detection limit of 49 nM. The sensing mechanism was considered that the hypochlorite triggered oxidative hydrolysis of probe HBM and the cyanobiphenyl fluorophore was released, which also have been evidenced by the MS analysis. Most notably, the response time of the probe HBM to the OCl − is only a few seconds. The probe was also successfully applied to fluorescence imaging of OCl − in the living cells. [ABSTRACT FROM AUTHOR]