1. A Phenanthrenequinone-Based Ratiometric Fluorescent Probe for Rapid Detection of Peroxynitrite with Imaging in Osteoblast Precursor Cells.
- Author
-
Karak A, Banik D, Ganguly R, Banerjee S, Ghosh P, Maiti A, Mandal D, and Mahapatra AK
- Subjects
- Molecular Structure, Optical Imaging, Limit of Detection, Animals, Humans, Spectrometry, Fluorescence, Fluorescent Dyes chemistry, Fluorescent Dyes chemical synthesis, Peroxynitrous Acid analysis, Osteoblasts drug effects, Phenanthrenes chemistry
- Abstract
In the current situation, peroxynitrite (ONOO
- ) is drawing the increasing attention of researchers for its pivotal role in diverse pathological and physiological processes on grounds of robust oxidation and nitrification. Herein, we have successfully designed and synthesized a phenanthrenequinone benzyl borate-based chemosensor for fast and selective detection of ONOO- . The probe PTDP itself had an orange fluorescence, which was changed to strong blue fluorescence upon the addition of ONOO- , indicating the ratiometric response of the probe. This is so because of the cleavage of the benzyl boronate-protecting group of PTDP upon the addition of ONOO- with simultaneous releasing of pyridinyl-based chemosensor PPI . The PTDP showed outstanding performance in the various photophysical studies such as good selectivity, excellent sensitivity with a very low detection limit of 2.74 nM, and a very fast response time (<15 s). Furthermore, for practical applicability, it was successfully applied in the ratiometric detection of ONOO- in osteoblast precursor cells.- Published
- 2024
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