1. Red‐ and Far‐Red‐Emitting Zinc Probes with Minimal Phototoxicity for Multiplexed Recording of Orchestrated Insulin Secretion.
- Author
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Zhang, Junwei, Peng, Xiaohong, Wu, Yunxiang, Ren, Huixia, Sun, Jingfu, Tong, Shiyan, Liu, Tianyan, Zhao, Yiwen, Wang, Shusen, Tang, Chao, Chen, Liangyi, and Chen, Zhixing
- Subjects
SECRETION ,INSULIN ,ZINC ,FLUORIMETRY ,ISLANDS ,RED - Abstract
Zinc biology, featuring intertwining signaling networks and critical importance to human health, witnesses exciting opportunities in the big data era of physiology. Here, we report a class of red‐ and far‐red‐emitting Zn2+ probes with Kd values ranging from 190 nM to 74 μM, which are particularly suitable for real‐time monitoring the high concentration of Zn2+ co‐released with insulin during vesicular secretory events. Compared to the prototypical rhodamine‐based Zn2+ probes, the new class exploits morpholino auxochromes which eliminates phototoxicity during long‐term live recording of isolated islets. A Si‐rhodamine‐based Zn2+ probe with high turn‐on ratio (>100), whose synthesis was enabled by a new route featuring late‐stage N‐alkylation, allowed simultaneous recording of Ca2+ influx, mitochondrial signal, and insulin secretion in isolated mouse islets. The time‐lapse multicolor fluorescence movies and their analysis, enabled by red‐shifted Zn2+ and other orthogonal physiological probes, highlight the potential impact of biocompatible fluorophores on the fields of islet endocrinology and system biology. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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