1. A two-photon fluorescent probe for HNO rapid visualization in endoplasmic reticulum.
- Author
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Peng, Shuxin, Li, Zipeng, Zhang, Yongru, Cao, Wenbo, Liu, Jianfei, Zhu, Weimin, and Ye, Yong
- Subjects
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ENDOPLASMIC reticulum , *FLUORESCENT probes , *REACTIVE nitrogen species , *VISUALIZATION , *DETECTION limit - Abstract
• A new two-photon fluorescent probe for HNO was developed. • The probe showed faster response time (2 min), lower detection limit (32 nm) and strong anti-interference ability. • The probe can target the ER and visualization of NO and H 2 S cross-talk on the ER for the first time. Nitroxyl (HNO) is a one-electron reduction product of nitric oxide (NO), which mainly exists in protonated form during physiological processes. As a kind of reactive nitrogen species (RNS), HNO has significant correlations with many physiological and pathological pathways. In this study, we report a two-photon fluorescent probe R2, which can detect HNO based on the reaction with a triarylphosphine group. Upon exposure to HNO, the probe R2 holds "naked eye" recognition, high selectivity and fast response (2 min). These excellent spectral properties promoted the detection of exogenous and endogenous HNO in MCF-7 cells. Furthermore, the probe could be selectively located in the endoplasmic reticulum (ER) with high biocompatibility and successfully apply to visualize NO/H 2 S cross-talk to generate HNO. These results support that R2 will provide a powerful tool to elucidate the physiological and pathological functions of HNO in the ER. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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