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• A new two-photon fluorescent probe for HNO was developed. • The probe showed faster response time (2 min), lower detection limit (32 nm) and strong anti-interference ability. • The probe can target the ER and visualization of NO and H 2 S cross-talk on the ER for the first time. Nitroxyl (HNO) is a one-electron reduction product of nitric oxide (NO), which mainly exists in protonated form during physiological processes. As a kind of reactive nitrogen species (RNS), HNO has significant correlations with many physiological and pathological pathways. In this study, we report a two-photon fluorescent probe R2, which can detect HNO based on the reaction with a triarylphosphine group. Upon exposure to HNO, the probe R2 holds "naked eye" recognition, high selectivity and fast response (2 min). These excellent spectral properties promoted the detection of exogenous and endogenous HNO in MCF-7 cells. Furthermore, the probe could be selectively located in the endoplasmic reticulum (ER) with high biocompatibility and successfully apply to visualize NO/H 2 S cross-talk to generate HNO. These results support that R2 will provide a powerful tool to elucidate the physiological and pathological functions of HNO in the ER. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • A new N-oxide based and turn on fluorescent probe for Fe2+ was developed. • The probe possessed a high selectivity for Fe2+ among various biologically relevant species and fast rapid response. • Monitoring Fe2+ with TPM fluorescence imaging for the first time. Abstract Iron is an essential element for human body, involved in important biochemical processes in the body, especially in biological oxidation. Misregulation of iron levels in the cell is concerned with serious diseases. Here, a novel two photon and turn on NIR-fluorescent probe based ICT for detection of Fe2+ is first reported. The probe exhibited brilliant selectivity and excellent sensitivity (DL = 4.5 μM), rapid response (15 min) and "naked-eye colorimetric sensor" for monitoring of Fe2+. More importantly, the probe was successfully applied to real-time monitor Fe2+ in living cell through two-photon microscopy fluorescence imaging with excitation 680 nm. [ABSTRACT FROM AUTHOR]

A ER-targetable probe R1 for HOCl was synthesized. R1 shows excellent selectivity and high sensitivity, excellent anti-interference, and can quickly reach the response platform. • A new ER-targetable ICT-based fluorescent probe R1 for HClO was developed. • Probe have rapid response (30 s), big Stock's shift and a wide range of pH(7–13). • The imaging in EC1 cells demonstrated its value of practical application. The endoplasmic reticulum is the largest organelle in the cell. Studies have shown that the concentration of HClO is related to many diseases. A new type of probe R1 has positioned to the endoplasmic reticulum. The probe shows good positioning effect, excellent selectivity and high sensitivity, excellent anti-interference, and can quickly reach the response platform. The obvious color change can be used for naked eye observation. Due to its low cytotoxicity, probe R1 was successfully used for EC1 cells imaging under a laser scanning confocal microscope. [ABSTRACT FROM AUTHOR]