Search

In this paper, the novel fluorescence probe XP based on Schiff-base was designed, synthesized and characterized, which could detect Y3+selectively and sensitively. The recognition mechanism of XP toward Y3+ was studied by Job's plot and HRMS. It was investigated that stoichiometric ratio of the probe XP conjugated with Y3+ was 1:2. And the detection limit was calculated as 0.30 μM. In addition, Y3+ was recognized by the test paper made from XP. And the probe XP could detect Y3+ selectively in Caenorhabditis elegans and the main organs of mice. Thus, XP was considered to have some potential for application in bioimaging. [ABSTRACT FROM AUTHOR]

Tin is a crucial trace element in the human body, playing a significant role in various physiological activities and overall human health. In this study, we present a novel fluorescent probe (CDM) capable of detecting Sn2+ through both colorimetric and fluorometric channels. The probe allows for visual detection by the naked eye and exhibits long-wavelength fluorescence emission. With a detection limit of 4.72 × 10−8 M and 9.95 × 10−9 M, respectively, CDM surpasses the World Health Organization's specified limit of 8.4 × 10−4 M and outperforms previously reported probes. As far as we know, this is the first fluorescent probe to enable naked-eye and long-wavelength emission-based detection of Sn2+. Additionally, a smartphone-based sensing platform was developed to recognize Sn2+. CDM not only demonstrates sensitive detection of Sn2+ in common food products but also successfully imaging Sn2+ in 4T1 cells, Caenorhabditis elegans, and mice. [Display omitted] • The probe to detect Sn2+ based on the dual-channel and long-wavelength emission was reported firstly. • The portable smartphone sensing platform to detect Sn2+ was produced. • The probe was successfully applied to bioimaging of food, C. elegans, mice and 4T1 cells. [ABSTRACT FROM AUTHOR]

[Display omitted] • The AXS probe can rapidly detect Zn2+ by near-infrared fluorescence. • Smartphone and fluorescent dual-model detection were achieved. • Qualitative and quantitative determination of Zn2+ in common foodstuffs. • Successful applications for bioimaging in Caenorhabditis elegans and mice. Zn2+ plays a vital role in regulating various life processes, such as gene expression, cell signaling, and brain function. In this study, a near-infrared fluorescent probe AXS was synthesized to detect Zn2+ with good fluorescence specificity, high selectivity, and high sensitivity; the detection limit of Zn2+ was 6.924 × 10−11 M. The mechanism of Zn2+ recognition by the AXS probe was investigated by 1H nuclear magnetic resonance titrations, UV–visible spectroscopy, fluorescence spectroscopy, Fourier-transform infrared spectroscopy, and high-resolution mass spectrometry. Test paper experiments showed that the AXS probe could detect Zn2+ in real samples. In addition, quantitative and qualitative detection of Zn2+ in common foodstuffs was achieved. For portable Zn2+ detection, a smartphone detection platform was also developed based on the AXS probe. Importantly, the AXS probe showed good bioimaging capabilities in Caenorhabditis elegans and mice. [ABSTRACT FROM AUTHOR]

Pb2+ is a heavy metal ion pollutant that poses a serious threat to human health and ecosystems. The conventional methods for detecting Pb2+ have several limitations. In this study, we introduce a novel fluorescent probe that enables the detection of Pb2+ in the near-infrared region, free from interference from other common ions. A unique characteristic of this probe is its ability to rapidly and accurately identify Pb2+ through ratiometric measurements accompanied by a large Stokes shift of 201 nm. The limit of detection achieved by probe was remarkably low, surpassing the standards set by the World Health Organization, and outperforming previously reported probes. To the best of our knowledge, this is the first organic small-molecule fluorescent probe with both near-infrared emission and ratiometric properties for the detection of Pb2+. We present a triple-mode sensing platform constructed using a probe that allows for the sensitive and selective recognition of Pb2+ in common food items. Furthermore, we successfully conducted high-quality fluorescence imaging of Pb2+ in various samples from common edible plants, HeLa cells, Caenorhabditis elegans , and mice. Importantly, the probe-Pb2+ complex exhibited tumour-targeting capabilities. Overall, this study presents a novel approach for the development of fluorescent probes for Pb2+ detection. [Display omitted] • This study develops a novel probe for ratiometric and NIR detection of Pb2+ for the first time. • A fluorescence/colorimetric/smartphone sensing platform for Pb2+ detection was fabricated. • The triple-mode sensing platform was successfully applied to the analysis of Pb2+ in various foods. • The probe was successfully applied to bioimaging and has tumour-targeting capabilities. [ABSTRACT FROM AUTHOR]

[Display omitted] • The probe toward CN− with large Stokes shift was firstly reported based on ESIPT-AIE. • The probe showed high selectivity, rapid response and the low limit of detection. • It was detect endogenous CN− in food samples and living system successfully. Cyanide (CN–) is one of the strongest and fastest-acting toxic substances, and its excessive presence will cause great harm to the environment, food and human health. Although several fluorescent probes that recognize CN– have been extensively reported, developing the fluorescent probe with aggregation-induced emission (AIE) and large Stokes shift is still an extremely difficult problem due to lack of effective strategies. Thus, continuing efforts to seek novel, structurally distinct and functionally specific structures with large Stokes shift and AIE features are highly anticipated. Herein, we design and synthesize a novel asymmetric bis-Schiff base block (named as HPBC) based on the excited state intramolecular proton transfer (ESIPT) and AIE mechanism. Benefiting from restriction of intramolecular rotation and –CN electron-withdraw nature promoting the ESIPT effect of HPBC in the recognition system, HPBC shows large Stokes shift (243 nm), high selectivity, rapid identification of CN– and AIE effects. Excitingly, the limit of detection (LOD) was calculated to be 1.32 × 10-7 M, which was much lower than the 1.9 × 10-6 M specified by the World Health Organization and better than other previously reported probes. More importantly, further investigations demonstrated that the molecule we designed was successfully applied to sense endogenous CN– in food samples and the low cytotoxicity of HPBC was allowed to discover CN– in C. elegans , mice and HeLa cells. Overall, this work provides a new viewpoint of the rational design and synthesis of fluorescent probe toward CN– with AIE and large Stokes shift features, and triggers the discovery of new functions and properties of biological materials, which would open a new frontier for probes. [ABSTRACT FROM AUTHOR]

Carbon monoxide (CO), a significant gas transmitter, plays a vital role in the intricate functioning of living systems and is intimately linked to a variety of physiological and pathological processes. To comprehensively investigate CO within biological system, researchers have widely adopted CORM-3, a compound capable of releasing CO, which serves as a surrogate for CO. It aids in elucidating the physiological and pathological effects of CO within living organisms and can be employed as a therapeutic drug molecule. Therefore, the pivotal role of CORM-3 necessitates the development of effective probes that can facilitate the visualization and tracking of CORM-3 in living systems. However, creating fluorescent probes for real-time imaging of CORM-3 in living species has proven to be a persisting challenge that arises from factors such as background interference, light scattering and photoactivation. Herein, the BNDN fluorescent probe, a brand-new near-infrared is proposed. Remarkably, the BNDN probe offers several noteworthy advantages, including a substantial Stokes shift (201 nm), heightened sensitivity, exceptional selectivity, and an exceedingly low CORM-3 detection limit (0.7 ppb). Furthermore, the underlying sensing mechanism has been meticulously examined, revealing a process that revives the fluorophore by reducing the complex Cu2+ to Cu+. This distinctive NIR fluorescence "turn-on" character, coupled with its larger Stokes shift, holds great promise for achieving high resolution imaging. Most impressively, this innovative probe has demonstrated its efficacy in detecting exogenous CORM-3 in living animal. It is important to underscore that these endeavors mark a rare instance of a near-infrared probes successfully detecting exogenous CORM-3 in vivo. These exceptional outcomes highlighted the potential of BNDN as a highly promising new tool for in vivo detection of CORM-3. Considering the impressive imaging capabilities demonstrated by BNDN presented in this study, we anticipate that this tool may offer a compelling avenue for shedding light on the roles of CO in future research endeavors. A novel NIR fluorescent probe BNDN for detection of CORM-3 in living cells and animalA novel robust NIR fluorescent probe for the detection of CORM-3 has been reported. The probe not only demonstrates remarkable selectivity towards CORM-3 over other potential interfering species, but it also exhibits high sensitivity to CORM-3, yielding 20-fold fluorescence enhancement. Particularly, the probe effectively tracks and images CORM-3 in vivo such as mice. These findings suggest that BNDN holds significant promise as a tool for exploring CO-related physiological and pathological processes in living systems. [Display omitted] • Reporting a novel probe for simultaneously detecting CORM-3 in living cells and animals. • This probe shows enhanced fluorescence in the NIR region with a large Stokes shift. • The underlying sensing mechanism has been meticulously examined. [ABSTRACT FROM AUTHOR]

Real-time and accurate detection of intracellular GSH content is crucial for clinical diagnosis, disease treatment, and pathogen detection. Creating fluorescent probes that possess aggregation induced emission (AIE) and precisely detect GSH remains a formidable challenge despite the widespread reporting of numerous fluorescent probes for GSH recognition. This may due to the absence of effective strategies. Herein, using a "turn off - turn on" strategy, a novel fluorescent probe DPND with AIE characteristic has been devised and synthesized. It can detect GSH selectively and uninhibitedly in the presence of various ions. The limit of detection of DPND -Cu2+ for GSH was calculated to be 1.17 μM, demonstrating the probe's ability to detect GSH quantitatively. More crucially, the probe exhibits low toxicity and solid biocompatibility while recognizing GSH in HeLa cells and mices, indicating that it may be a promising candidate for in vivo detection of GSH. Consequently, this work offers the potential to precisely tailor molecular recognition of GSH in addition to providing ongoing exploration of approaches for monitoring GSH. • A novel and convenient strategy for detecting GSH with high sensitivity, selectivity and low detection limit. • It is quite rare for AIE-based fluorescent probes for detecting GSH. • The probe was demonstrated in various exogenous cells, presenting excellent cell biocompatibility in living cells. [ABSTRACT FROM AUTHOR]