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Biothiols play an important role in cell protein metabolism and signal transduction, especially Hcy was considered as a new risk factor closely related to many diseases. However, there remain some limitations in selectively detecting Hcy due to its structural similarity to Cys. It is urgent for us to develop a novel indicator to study the pathological process of Hcy. Herein, we constructed a new fluorescent probe TCF-SBD composed of tricyanofuran fluorophore and SBD reaction site for detecting Hcy. The probe TCF-SBD exhibited a strong yellow emission response to Hcy in an "off-on" manner, and showed good selectivity for Hcy over Cys and GSH. The sensing mechanism was confirmed by HPLC, NMR titration and DFT studies. The probe was sensitive to the detection of Hcy with LOD low to 0.23 μM. The powerful probe TCF-SBD succeeded in real-time and selectively visualizing Hcy in living cells. [Display omitted] • Fluorescence probe based on substitution-rearrangement process was synthesized for selective detection of Hcy. • The probe displayed high selective and sensitive for Hcy (detection limit is 0.23 μM). • The probe was applied for fluorescence imaging of exogenous Hcy in living cells. [ABSTRACT FROM AUTHOR]

[Display omitted] • A simple naphthalimide-based chemosensor was synthesized based on ICT process for ratiometric detection of SO 2 derivatives. • The probe displayed high selective and sensitive for derivatives (detection limit is 1.53 μM). • The probe was applied for exogenous and endogenous SO 2 imaging by dual emission channels in living Hela and HepG2 cells. SO 2 is a well-known signal molecule and one of reactive sulfur species, which closely participates in many metabolic processes. While unbalanced metabolism of sulfur dioxide can lead to serious complications of various diseases. Therefore, a rapid and accurate monitoring of SO 2 derivatives with high selectivity and sensitivity would be beneficial for their bio-analytic studies. Herein, a novel ratiometric fluorescent probe (NG-TCF) based on ICT mechanism for monitoring SO 2 was developed. The probe underwent a nucleophilic addition of HSO 3 -/SO 3 2- to give rise to a 120 nm blue-shift dual-emission signal changes in enhanced green channel and subdued red channel under a single wavelength excitation. The probe showed fast response rate (within 7 min), good sensitivity (the detection limit is 1.53 µM), and specific response toward HSO 3 -/SO 3 2- over other bio-species, including H 2 S and ClO-. Moreover, the probe can be applied for visual ratio imaging of exogenous and endogenous SO 2 derivatives in living cells. [ABSTRACT FROM AUTHOR]