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2 results on '"Selected MS/MS ion monitoring (SMIM)"'

1. Rapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometry

Author

Carrera, Mónica, Cañas, Benito, and Gallardo, José M.

Subjects
*FISHES, *PARVALBUMINS, *MASS spectrometry, *ALLERGENS, *HIGH-intensity focused ultrasound, *TRYPSIN, *METHODOLOGY
Abstract

Abstract: Parvalbumins beta (β-PRVBs) are considered the major fish allergens. A new strategy for the rapid and direct detection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of β-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen β-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the direct detection of the presence of fish β-PRVBs in any food product in less than 2 hours. [Copyright &y& Elsevier]

Published
2012
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2. Rapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometry

Author

Mónica Carrera, José Manuel Gallardo, and Benito Cañas

Subjects
Proteomics, PRVB, Fish Proteins, Parvalbumins, Biophysics, medicine.disease_cause, Mass spectrometry, Biochemistry, Focused ultrasound, Mass Spectrometry, Ion, Allergen, medicine, Animals, Humans, Quadrupole ion trap, Selected MS/MS ion monitoring (SMIM), Chromatography, biology, Marine, Chemistry, Fishes, Allergens, Fish, Seafood, Food, biology.protein, %22">Fish , High intensity focused ultrasound (HIFU), Parvalbumin, Food Analysis, Food Hypersensitivity
Abstract

10 páginas, 5 figuras, 2 tablas, Parvalbumins beta (β-PRVBs) are considered the majorfishallergens. A new strategy for the rapid and directdetection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of β-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen β-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the directdetection of the presence of fish β-PRVBs in any food product in less than 2 hours. This new affordable assay would be very useful for the sanitary and inspect authorities to protect sensitive consumers from allergic reactions and to guarantee their safety, This work was supported by the Comisión Interministerial de Ciencia y Tecnología (CICyT) (Project AGL2000-0440-P4-02). Dr Mónica Carrera is supported by the Barrié Fundation

Published
2011

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