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Blood cockle (Anadara granosa) is one of the marine biotas that can be used as a bioindicator of the pollution level of sea water. The nature of blood cockle stays in one place because of their slow movement and they are non-selective filter feeders which filter water in order to get food. The use of activated charcoal during soaking is to keep the food safety from blood cockle contaminated with heavy metals. The purpose of this study is to determine the effectivity test of soaking duration on blood cockle (Anadara granosa) and activated charcoal toward reducing metals lead (Pb). This study employed Randomized Complete Design (RCD) using three different soaking periods such as 30 minutes, 60 minutes, and 90 minutes. The samples were analyzed by Inductively Coupled Plasma Emission (ICPE). The parametric data was analyzed with One Way Anova test. The result of the study showed that the soaking duration among 30 minutes, 60 minutes, and 90 minutes in activated charcoal showed significantly different (P< 0.05) toward the levels of lead.

Metabolite profiles of plant organs and their in vitro cultures of Justicia gendarussa have been studied by using Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight-Mass Spectrometry (UPLC-Qtof-MS). Samples of leaves, stems, roots, and shoot cultures showed similar patterns of metabolites, while samples of root cultures gave very different profiles. Concentrations of secondary metabolites in shoot cultures were relatively low compared to those in the leaves and roots of the plants. The results suggested that secondary metabolites in J. gendarussa were biosynthetized in the leaves, then transported to the roots.