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The committee has been quite active since the 2001 AOAC Meeting and Exposition. Five collaboratives in various stages are progressing toward committee approval. First was the Alternative Methanol Extraction Procedure for Method 2001.12; on June 13 a conference call was held to discuss and ratify Method 2002.04, Gravimetric Determination of Amylase Treated Neutral Detergent Fiber in Feeds Using Refluxing Beakers or Crucibles. Currently the committee is processing the following collaboratives: IC-017, Crude Fat, Diethyl Ether Extraction in Feed, Cereal Grain, and Forage (Randall/Soxtec/Submersion Method); IC28 Crude Fat, Hexane Extraction in Feed, Cereal Grain, and Forage (Randall/Soxtec/Submersion Method); and IC29, Determination by Liquid Chromatography of Urea in Certain Liquid Fertilizers Containing Substantial Amounts of Water-Soluble Urea-Formaldehyde Reaction Products and in Aqueous Urea Solutions. The goal is to have all reviews completed, reviews by the 2 designated OMB members, and the committee vote set for September at the Committee meeting in Los Angeles.

Cyanide-resistant O(2) consumption can be stimulated by either treating whole white potato tubers (Norchip) with ethylene, in the presence of 100% O(2), or aging slices obtained from untreated potato tubers. A comparison of alternative pathway activity elicited by either treatment was undertaken. The proportion of electrons flowing through the alternative path in the presence of intermediate concentrations of KCN and at various concentrations of salicylhydroxamic acid was identical in both cases. However, the respiration of slices from ethylene-treated tubers was in every case stimulated by KCN, whereas the aged slices never exhibited this phenomenon. Furthermore, the metabolism of d-[U-(14)C]glucose was several hundred times greater in aged slices than in fresh slices from C(2)H(4)-treated tubers. These results, along with the respiratory kinetics of aged slices from ethylene-treated tubers, suggest that aged slices and fresh slices from ethylene-treated tubers are biochemically dissimilar.