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The purpose of this paper is to introduce a promising, novel method to aid in the assessment of bone quality in forensically relevant skeletal remains. BMD is an important component of bone's nutritional status and in skeletal remains of both juveniles and adults, and it can provide information about bone quality. For adults remains, it can provide information on pathological conditions or when bone insufficiency may have occurred. In juveniles, it provides a useful metric to elucidate cases of fatal starvation or neglect, which are generally difficult to identify. This paper provides a protocol for the anatomical orientation and analysis of skeletal remains for scanning via dual-energy X-ray absorptiometry (DXA). Three case studies are presented to illustrate when DXA scans can be informative to the forensic practitioner. The first case study presents an individual with observed longitudinal fractures in the weight bearing bones and DXA is used to assess bone insufficiency. BMD is found to be normal suggesting another etiology for the fracture pattern present. The second case study employed DXA to investigate suspected chronic malnutrition. The BMD results are consistent with results from long bone lengths and suggest the juvenile had suffered from chronic malnutrition. The final case study provides an example where fatal starvation in a fourteen-month infant is suspected, which supports autopsy findings of fatal starvation. DXA scans showed low bone mineral density for chronological age and is substantiated by traditional assessments of infant health. However, when dealing with skeletal remains taphonomic alterations should be considered before applying this method.

Simple Summary: Understanding bone diagenesis, or alteration, in juvenile and fetal remains has important implications for forensic science. First, it can suggest information about the deposition of the remains and a possible postmortem interval (PMI). Second, it can assist in evaluating bone integrity and the potential for molecular testing of these remains for forensic purposes. This study investigates how early bone diagenesis is observed in fetal and juvenile mammalian remains as well as differences in degradation based on the deposition of the remains (e.g., blanket wrapping, shallow burial, etc.). We found that there were differences in the extent of bone diagenesis between depositions, with bagged remains exhibiting relatively less degradation over time than the other three depositions, while buried remains exhibited the greatest extent of degradation over time. However, all the remains showed bone diagenesis regardless of time of interment or deposition, with all remains exhibiting alteration as early as three months. This is consistent with adult remains, although the presentation of alteration differs and is likely related to developmental differences between subadult and adult bone. It is well understood that intrinsic factors of bone contribute to bone diagenesis, including bone porosity, crystallinity, and the ratio of organic to mineral components. However, histological analyses have largely been limited to adult bones, although with some exceptions. Considering that many of these properties are different between juvenile and adult bone, the purpose of this study is to investigate if these differences may result in increased degradation observed histologically in fetal and juvenile bone. Thirty-two fetal (n = 16) and juvenile (n = 16) Sus scrofa domesticus femora subject to different depositions over a period of two years were sectioned for histological observation. Degradation was scored using an adapted tunneling index. Results showed degradation related to microbial activity in both fetal and juvenile remains across depositions as early as three months. Buried juvenile remains consistently showed the greatest degradation over time, while the blanket fetal remains showed more minimal degradation. This is likely related to the buried remains' greater contact with surrounding soil and groundwater during deposition. Further, most of the degradation was seen in the subendosteal region, followed by the subperiosteal region, which may suggest the initial microbial attack is from endogenous sources. [ABSTRACT FROM AUTHOR]

One of the goals of the Forensic Anthropology Society of Europe (FASE) is to map the existing education and practice opportunities in the field of forensic anthropology in order to support the development of the discipline and to optimize the training courses provided by the Society. To address this goal, an online questionnaire was sent to European and South African practitioners of forensic anthropology and related disciplines in 2016. The results of the questionnaire showed that the status and roles of forensic anthropologists vary depending on the national legal systems, education, and employment status of the practitioners. Despite the fact that the expertise of forensic anthropologists has been increasingly requested in a variety of investigations and the spectrum of tasks has become broader, including identification of living persons, specialized education in forensic anthropology is still restricted to a few graduate and postgraduate programs in European countries and to annual FASE courses. [ABSTRACT FROM AUTHOR]

Estimating age‐at‐death of individuals represented only by skeletonized human remains is a fundamental aspect of forensic anthropological casework. Recently, several researchers have proposed that bone mineral density (BMD) is a useful predictor of age‐at‐death in forensic contexts. Navega et al. (JFS 63(2):497–503) developed an online application called DXAGE for calculating age‐at‐death from BMD parameters. This study tests the utility of DXAGE by utilizing data from the National Health and Nutrition Examination Survey (NHANES). BMD data from a female subsample (n = 470) of the NHANES 2007–2008 dataset were analyzed, and the relationship between predicted age and real age was examined. Inaccuracy was 14.25 years, and bias was −7.20 years. Results show that there is a weak correlation between predicted and actual age (r = 0.47) using the DXAGE application. While BMD data are potentially useful for predicting age age‐at‐death, the DXAGE application should be used cautiously in forensic anthropological contexts. [ABSTRACT FROM AUTHOR]

There is currently a dearth of research investigating the progression and rate of decomposition for juvenile remains. It is thought that juveniles and infants decompose at an increased rate relative to adults due simply to body mass and that skeletal preservation is commonly dependent on intrinsic levels of bone mineral density (BMD). This study investigates the environmental variables important in driving juvenile decomposition as well as examining if currently accepted methodology for quantifying adult decomposition can be applied to juvenile remains. Furthermore, histological analysis is undertaken to test the Histological Index (HI) as a semi-quantitative indicator of decomposition. Thirty-five Sus scrofa ranging between 1.8 and 22.7 kg were deposited to simulate body mass of human infant and juvenile remains. Pigs were deposited every season over two years in the southeastern US with five depositional types: bagged, blanket wrapped, and surface control foetal remains, surface, and buried juvenile remains. Remains were scored quantitatively throughout soft tissue decomposition. Following study completion and skeletonization, a femur was selected from each set of remains for histological analysis. Thick sections were assessed under standard brightfield light and scored using Oxford Histological Index (OHI). Results indicate that seasonal variation is an important factor to consider even when using a standardized time variable such as accumulated degree days (ADD), particularly variation in soil moisture. Soil moisture was a consistent significant variable in the mixed effects model. The pattern of decomposition using total body score (TBS) was similar to that observed by others prior to log transformation with a rapid incline early in decomposition with levelling off. The correlation between time in days, ADD, and TBS was not as strong as those previously reported (R2 = 0.317 and 0.499, respectively) suggesting that TBS as it is currently formulated cannot be directly applied to juvenile remains. Finally, the OHI model performed moderately well, but was variable even within seasons across multiple years. [ABSTRACT FROM AUTHOR]

Abstract: The structural breakdown of skeletal remains follows a lengthy decomposition process, progressing from the appearance of cracking along the bone to complete loss of shape and skeletal integrity, that can occur in as early as 6 years or as long as 30. The length of time a set of skeletal remains takes to decompose depends heavily upon its micro-environment, the local context in which decomposition agents act on remains. Critical changes in the micro-context can drastically increase or slow the process. The burial of remains below the surface has been found to effectively shut out many of the most destructive decaying agents, such as the natural elements and insect and animal activity. This causes an increase in the amount of time needed to breakdown the skeletal remains. A better understanding of the decomposition process in varying micro-contexts is important for the ability to correctly estimate time since burial. This can aid not only in understanding of archaeological remains, but also in the identification of individuals in modern forensic and human rights cases. This study details the observations made during the excavation and analysis of burials on the Isla de Coiba off the coast of Panama. The excavation took place during the summer 2003 in an effort to identify several individuals who were believed to be part of the ‘disappeared’, people who went missing and believed to have been murdered under orders by the Torrijos and Noriega military regimes. Through the fieldwork analysis a decomposition timeline, specific for the Coiba micro-environment, was created. This new set of stages can be used to estimate time since burial in other areas whose local context is similar to that found on Coiba. [ABSTRACT FROM AUTHOR]

Sex determination of the human skeleton is best assessed from the os coxa. The present study explored the possibility of using three-dimensional landmark coordinate data collected from various landmarks located over the entire bone to determine whether there were significant sex differences local to the landmarks. Thirty-six landmarks were digitized on 200 African American and European American male and female adult human os coxae. MANCOVA results show that sex and size have a significant effect on shape for both European Americans (Sex, F = 17.50, d.f. = 36, 63, p > F = 0.0001; Size, F = 2.56, d.f. = 36, 63, p > F = 0.0022) and African Americans (Sex, F = 21.18, d.f. = 36, 63, p > F = 0.0001; Size, F = 2.59, d.f. = 36, 63, p > F = 0.0005). The discriminant analysis shows that sexing accuracy for European Americans is 98% for both males and females, 98% for African American females, and 100% for African American males. [ABSTRACT FROM AUTHOR]

The Johns Hopkins University Center for Craniofacial Development and Disorders estimates that 1 in 3,000 children born in the United States is diagnosed with a rare form of craniosynostosis. Although the medical literature has documented numerous descriptions of craniofacial disorders from an anthropometric or genetic perspective, considerably fewer reports of these anomalies have been documented in the context of forensic anthropology. Similar genetic origins of many craniofacial anomalies generate ranges of phenotypic variation between and even within documented cases, producing difficulties in acquiring correct diagnoses. Identical physical characteristics manifested in different disorders create further complications in identifying a craniofacial syndrome in skeletal remains. Reported here is an unusual case of a possibly undiagnosed craniofacial abnormality in a set of identified skeletal remains from a North Carolina homicide case. Traditional metric and geometric morphometric approaches were utilized to further investigate morphological shape differences between the case study and a reference sample. Results show significant differences suggesting a nonsyndromic form of craniosynostosis. [ABSTRACT FROM AUTHOR]

This study evaluates issues of precision, repeatability, and validation in three-dimensional (3D) landmark coordinates. Two observers collected 19 homologous cranial landmarks from three skulls during three separate digitizing sessions. Statistical analysis was conducted on the 171 interlandmark distances (ILDs) derived from the original coordinate data. A mixed model ANOVA detected significant within-subject error in 54 of the 171 ILDs (i.e., 32%). A GLM procedure revealed significant between-observer variation in 14 ILDs and significant observer-by-session differences in 13 ILDs. The majority of these differences involved ILDs with type 3 landmarks as endpoints, such as euryon and alare. Unlike type 1 and 2 landmarks which are biologically informative in all directions, type 3 landmarks contain a substantial arbitrary component. Thus, it is not surprising type 3 landmarks displayed significant digitizing error. Given these results, we caution researchers to be mindful of type 3 landmarks measurement discrepancies when selecting landmarks for coordinate data evaluation. [ABSTRACT FROM AUTHOR]

Numerous studies have addressed sex estimation from the hands and feet with varying results. These studies have utilized multiple measurements to determine sex from the hands and feet, including measures of robusticity (e.g., base width and midshaft diameter). However, robusticity measurements are affected by activity, which can disguise underlying patterns of sexual dimorphism. The purpose of this study is to investigate the utility of length measurements of the hands and feet to estimate sex. The sample consists of white females ( n=123) and males ( n=136) from the Terry Collection. Discriminant function analysis was used to classify individuals by sex. The left hand outperformed both the right hand and foot producing correct classification rates exceeding 80%. Surprisingly, the phalanges were better sex discriminators than either the metacarpals or metatarsals. This study suggests that length measures are more appropriate than robusticity measures for sex estimation. [ABSTRACT FROM AUTHOR]

Presents a pilot study of the among-sample morphological variation of modern 19th Cubans, Precontact Cubans, 17th Century Spanish and Terry Blacks using landmarks-based Procrustes analysis from geometric morphometrics. Biological variation of Cuban Americans which will facilitate the development of identification criteria specific for the U.S. hybrid Hispanic community; Conclusion that the application of available traditional craniometric and non-metric methods are not appropriate for the Latin population in South Florida.

Simple Summary: Within the practice of forensic anthropology ancestry is oftentimes used as a proxy for social race. This concept and its implications were explored via a content analysis (2009–2019) of the Journal of Forensic Sciences. Our findings revealed antiquated views of race based on the trifecta of continental populations (Asia, Europe, and Africa) continue to be pervasive in the field despite scientific invalidation of the concept of race decades earlier. Moreover, our employment of modern geometric morphometric and spatial analysis methods on craniofacial coordinate anatomical landmarks from several Latin American samples produced results in which the groups were not patterned by ancestry trifecta. Based on our findings we propose replacing the assumption of continental ancestry with a population structure approach that combines microevolutionary and cultural factors with historical events in the examination of population affinity. One of the parameters forensic anthropologists have traditionally estimated is ancestry, which is used in the United States as a proxy for social race. Its use is controversial because the biological race concept was debunked by scientists decades ago. However, many forensic anthropologists contend, in part, that because social race categories used by law enforcement can be predicted by cranial variation, ancestry remains a necessary parameter for estimation. Here, we use content analysis of the Journal of Forensic Sciences for the period 2009–2019 to demonstrate the use of various nomenclature and resultant confusion in ancestry estimation studies, and as a mechanism to discuss how forensic anthropologists have eschewed a human variation approach to studying human morphological differences in favor of a simplistic and debunked typological one. Further, we employ modern geometric morphometric and spatial analysis methods on craniofacial coordinate anatomical landmarks from several Latin American samples to test the validity of applying the antiquated tri-continental approach to ancestry (i.e., African, Asian, European). Our results indicate groups are not patterned by the ancestry trifecta. These findings illustrate the benefit and necessity of embracing studies that employ population structure models to better understand human variation and the historical factors that have influenced it. [ABSTRACT FROM AUTHOR]