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Your search keyword '"Mallavialle, Aude"' showing total 2 results
Start Over Author "Mallavialle, Aude" Topic forkhead transcription factors
2 results on '"Mallavialle, Aude"'

1. Forkhead box O3 (FOXO3) transcription factor mediates apoptosis in BCG-infected macrophages.

Author

Haoues M, Refai A, Mallavialle A, Barbouche MR, Laabidi N, Deckert M, and Essafi M

Subjects
Apoptosis genetics, Blotting, Western, Cell Line, Tumor, Forkhead Box Protein O3, Forkhead Transcription Factors genetics, Humans, Reverse Transcriptase Polymerase Chain Reaction, Apoptosis physiology, Forkhead Transcription Factors metabolism, Macrophages microbiology, Mycobacterium bovis physiology
Abstract

Enhanced apoptosis of BCG-infected macrophages has been shown to induce stronger dendritic cell-mediated cross-priming of T cells, leading to higher protection against tuberculosis (TB). Uncovering host effectors underlying BCG-induced apoptosis may then prove useful to improve BCG efficacy through priming macrophage apoptosis. Her we report that BCG-mediated apoptosis of human macrophages relies on FOXO3 transcription factor activation. BCG induced a significant apoptosis of THP1 (TDMs) and human monocytes (MDMs)-derived macrophages when a high moi was used, as shown by annexin V/7-AAD staining. BCG-induced apoptosis was associated with dephosphorylation of the prosurvival activated threonine kinase (Akt) and its target FOXO3. Cell fractionation and immunofluorescence microscopy showed translocation of FOXO3 to the nucleus in BCG-infected cells, concomitantly with an increase of FOXO3 transcriptional activity. Moreover, FOXO3 expression knock-down by small interfering RNA (siRNA) partially inhibited the BCG-induced apoptosis. Finally, real-time quantitative PCR (qRT-PCR) analysis of the expression profile of BCG-infected macrophages showed an upregulation of two pro-apoptotic targets of FOXO3, NOXA and p53 upregulated modulator of apoptosis (PUMA). Our results thus indicate that FOXO3 plays an important role in BCG-induced apoptosis of human macrophages and may represent a potential target to improve vaccine efficacy through enhanced apoptosis-mediated cross-priming of T cells., (© 2014 John Wiley & Sons Ltd.)

Published
2014
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2. Forkhead box O3 (FOXO3) transcription factor mediates apoptosis in BCG-infected macrophages

Author

Haoues, Meriam, Refai, Amira, Mallavialle, Aude, Barbouche, Mohamed-Ridha, Laabidi, Nizar, Deckert, Marcel, Essafi, Makram, Université de Tunis El Manar (UTM), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), and This work was supported by funds from the United Nations Development Program/World Bank/World Health Organization Special Program for Research and Training in Tropical Diseases (TDR), TDR ID No.A70529 and funds from the Tunisian Ministry for Higher Education, Research, and Technology. MH is a recipient of a two months travel/training fellowship from Pasteur International network (RIIP).

Subjects
Reverse Transcriptase Polymerase Chain Reaction, [SDV]Life Sciences [q-bio], Cell Line, Tumor, Macrophages, Blotting, Western, Forkhead Box Protein O3, Humans, Apoptosis, Forkhead Transcription Factors, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Mycobacterium bovis
Abstract

International audience; Enhanced apoptosis of BCG-infected macrophages has been shown to induce stronger dendritic cell-mediated cross-priming of T cells, leading to higher protection against tuberculosis (TB). Uncovering host effectors underlying BCG-induced apoptosis may then prove useful to improve BCG efficacy through priming macrophage apoptosis. Her we report that BCG-mediated apoptosis of human macrophages relies on FOXO3 transcription factor activation. BCG induced a significant apoptosis of THP1 (TDMs) and human monocytes (MDMs)-derived macrophages when a high moi was used, as shown by annexin V/7-AAD staining. BCG-induced apoptosis was associated with dephosphorylation of the prosurvival activated threonine kinase (Akt) and its target FOXO3. Cell fractionation and immunofluorescence microscopy showed translocation of FOXO3 to the nucleus in BCG-infected cells, concomitantly with an increase of FOXO3 transcriptional activity. Moreover, FOXO3 expression knock-down by small interfering RNA (siRNA) partially inhibited the BCG-induced apoptosis. Finally, real-time quantitative PCR (qRT-PCR) analysis of the expression profile of BCG-infected macrophages showed an upregulation of two pro-apoptotic targets of FOXO3, NOXA and p53 upregulated modulator of apoptosis (PUMA). Our results thus indicate that FOXO3 plays an important role in BCG-induced apoptosis of human macrophages and may represent a potential target to improve vaccine efficacy through enhanced apoptosis-mediated cross-priming of T cells.

Published
2013
Full Text
View/download PDF

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