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This esearch was performed aimed to study the biochemical parameters of the pond frog (Pelophylax lessonae) blood, from the populations inhabiting three biotopes of the Kazan city. Individuals of the pond frog Pelophylax lessonae were caught in July 2018 in three biotopes: a secondary reservoir near the Victory Park in Kazan, the Maloye Glubokoe karst lake and the Krugloye lake, which is a conditionally control area (a reserve). Determination of blood biochemical parameters included the analysis of glucose, lactate, total protein and hemoglobin. Two indicators were studied among the hematological signs: the content of erythrocytes and leukocytes. A lower content of glucose, total protein and an increased level of lactate were found in pond frogs from the population of the city center (Victory Park) in comparison with the conditionally control area and the "green zone" of the city. There was low hemoglobin content among the frogs from urban areas. The individuals from the P. lessonae population living in the Victory Park reservoir (urban population) showed a state of hypoglycemia, an increased blood lactate content, total protein, and hemoglobin decrease, and the increase of specific number of erythrocytes, which may indicate the conditions of hypoxia caused by high level of pollution of urban habitats and eutrophication of water bodies. Probably, various adaptive reactions are observed in anthropogenically disturbed amphibian habitats, consisting in erythrocyte number increase in response to the hemoglobin level decrease and also the change in the metabolic process intensity. [ABSTRACT FROM AUTHOR]