Search

Your search keyword '"Streptomyces murinus"' showing total 4 results
Start Over Descriptor "Streptomyces murinus" Topic fructose
4 results on '"Streptomyces murinus"'

1. Microwave irradiation-assisted isomerization of glucose to fructose by immobilized glucose isomerase

Author

Chuanming Wang, Ludong Liu, Aijun Zhang, Xuexun Fang, Li Tian, Hao Wu, and Dahai Yu

Subjects
Glucose-6-phosphate isomerase, Inorganic chemistry, Bioengineering, Fructose, Applied Microbiology and Biotechnology, Biochemistry, chemistry.chemical_compound, chemistry, Yield (chemistry), Irradiation, Magnesium ion, Isomerization, Microwave, Streptomyces murinus, Nuclear chemistry
Abstract

The isomerization of glucose to fructose by immobilized glucose isomerase (IGI, produced from Streptomyces murinus and immobilized on silica) was conducted under two different conditions—microwave irradiation and conventional heating to compare their overall effects. It was found that compared with conventional heating, microwave irradiation significantly enhanced the activity of IGI and the effects of acceleration might be non-thermal microwave effect. Under the optimum conditions of microwave irradiation-assisted isomerization (magnesium ion concentration of 50 mM, 0.1 M pH 7.5 phosphate buffered saline (PBS) buffer, 70 °C, microwave power of 480 W, agitation speed of 150 rpm, glucose concentration of 0.8 M and 2% IGI based on the solution weight percentage), 45% yield of fructose could be achieved in 16 h, while only 43% yield of fructose was obtained until 24 h by using conventional heating. These results indicated that microwave irradiation might be a fast and efficient method for enzyme-catalyzed isomerization of glucose to fructose.

Published
2011
Full Text
View/download PDF

2. Safety evaluation of the food enzyme glucose isomerase from Streptomyces murinus (strain NZYM‐GA).

Author

Silano, Vittorio, Barat Baviera, José Manuel, Bolognesi, Claudia, Brüschweiler, Beat Johannes, Cocconcelli, Pier Sandro, Crebelli, Riccardo, Gott, David Michael, Grob, Konrad, Lampi, Evgenia, Mortensen, Alicja, Rivière, Gilles, Steffensen, Inger‐Lise, Tlustos, Christina, Van Loveren, Henk, Vernis, Laurence, Zorn, Holger, Glandorf, Boet, Herman, Lieve, Marcon, Francesca, and Penninks, André

Subjects
*GLUCOSE isomerase, *STREPTOMYCES, *FRUCTOSE, *GENETICALLY modified foods, *GENETIC toxicology
Abstract

The food enzyme is a glucose isomerase (d‐xylose aldose‐ketose‐isomerase; EC 5.3.1.5) produced with a non‐genetically modified Streptomyces murinus strain NZYM‐GA by Novozymes A/S. The glucose isomerase is intended only to be used in an immobilised form in glucose isomerisation for the production of high fructose syrups. Residual amounts of total organic solids are removed by the purification steps applied during the production of high fructose syrups using the immobilised enzyme; consequently, dietary exposure was not calculated. Genotoxicity tests did not raise a safety concern. Similarity of the amino acid sequence to those of known allergens was searched and no match was found. The Panel considered that, under the intended conditions of use, the risk of allergic sensitisation and elicitation reactions by dietary exposure cannot be excluded, but the likelihood to occur is considered to be low. Based on the data provided, the immobilisation process and the removal of total organic solids during the production of high fructose syrups, the Panel concluded that this food enzyme does not give rise to safety concerns under the intended conditions of use. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

3. Glucose isomerase (EC 5.3.1.5) as a reagent in carbohydrate synthesis: success and failures with the isomerisation of non-natural derivatives of d

Author

Christian W. Ekhart, Michael Ebner, Arnold E. Stütz, A. De Raadt, A. Lechner, M. Fechter, and M. Strobi

Subjects
Xylose isomerase, Glucose-6-phosphate isomerase, Stereochemistry, Ketose, Carbohydrate synthesis, Fructose, General Chemistry, Isomerase, Catalysis, chemistry.chemical_compound, chemistry, Organic chemistry, Intramolecular Oxidoreductases, Streptomyces murinus
Abstract

The properties of glucose isomerase (EC 5.3.1.5) and its synthetic applications are briefly reviewed and recent results with non-natural substrates are discussed. Commercially available immobilized glucose isomerase from Streptomyces murinus, previously discovered to accept d -glucose as well as d -fructose derivatives with modifications at C-3, C-5, and C-6, was also found to be an efficient catalyst for the conversion of 5,6-dimodified derivatives of d -glucose as well as 5-modified d -xylofuranose into the corresponding open-chain 2-ketoses. The equilibrium mixture in all but one case investigated favoured the respective ketose in ratios of 3:1 to 4:1. These results are in agreement with molecular modelling experiments suggesting that the free energies of the furanoid aldoses serving as starting materials are generally slightly higher than the values for the corresponding open-chain ketose. Dimerisation of the open-chain sugar derivatives was not observed.

Published
1994
Full Text
View/download PDF

4. A New Immobilized Glucose Isomerase with High Productivity Produced by a Strain ofStreptomyces murinus

Author

O. B. Jørgensen, Susanne Rugh, S. Pedersen, L. G. Karlsen, and N. B. Nielsen

Subjects
Glucose-6-phosphate isomerase, Immobilized enzyme, biology, Streptomycetaceae, Organic Chemistry, Fructose, Isomerase, biology.organism_classification, Streptomyces, chemistry.chemical_compound, chemistry, Biochemistry, Actinomycetales, Streptomyces murinus, Food Science
Abstract

Description des proprietes biochimiques et fonctionnelles de cette nouvelle enzyme utilisee en fabrication de sirop de glucose a forte teneur en fructose

Published
1988
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results