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Mangaba (Hancornia speciosa Gomes) is a native fruit of Brazil that has high antioxidant activity and may benefit human health. The goal of this study was to discriminate between accessions in the ...

Made available in DSpace on 2021-06-25T11:03:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01 Using microsatellite loci, we assessed the mating system and genetic diversity of the dioecious tropical tree Genipa americana in a natural population (NP) and a progeny test (PT). For NP, we also estimated the paternity correlation within and among fruits and mean pollen dispersal distance. As expected for dioecious species, all offspring originated from outcrossing (t = 1). Mating among relatives (1-ts) and paternity correlation (rp) were variable among progenies (1-ts = 0.03-0.19; rp = 0.04-0.40), but greater in NP than in PT. Fixation index (F) was generally significant and lower in adults than in offspring, indicating selection against inbred individuals. Paternity correlation was higher within (0.40) than among (0.26) fruits, indicating a lower effective number of pollen donors (Nep) within (2.5) than among (3.8) fruits. Due to the higher rp in NP, the effective size within progenies (Ne) was lower (2.69) than PT (3.27). The pollen dispersal pattern was strongly leptokurtic, suggesting long-distance pollen dispersal (mean of 179 m). The results show that both populations can be used for seed collection in environmental reforestation programmes; however, considering that PT is structured in maternal progenies, NP is more suitable for seed collection due to the lower probability of mating among related trees. Universidade Federal Do Alagoas Campus de Engenharias e Ciências Agrárias Instituto Florestal de São Paulo, C.P. 1322 Universidade Estadual Paulista Instituto de Biotecnologia Unesp Embrapa Tabuleiros Costeiros Cia Energética de São Paulo Usina Porto Primavera Universidade Estadual Paulista Faculdade de Engenharia Unesp Universidade Estadual Paulista Instituto de Biotecnologia Unesp Universidade Estadual Paulista Faculdade de Engenharia Unesp

Mangaba (Hancornia speciosa Gomes) is a fruit species native to Brazil, belonging to the Apocynaceae family. Its cultivation is predominantly extractivist and its antioxidant properties have been recently reported. The objective of this study was to evaluate the bioactive compounds and antioxidant activity in fruits of accession from the Active Germplasm Bank of Embrapa Coastal Tablelands (BI, CA, LG, PR, PT and TC). The extracts were analyzed by high-performance liquid chromatography (HPLC), which identified chlorogenic acid (93.71 - 131.66 mg.100g-1), ferulic acid (0.85 - 2.27 mg.100g-1) and rutin (238.59 -442.94 mg.100g-1). The accessions CA and PR showed the highest values of total phenols (1179.39 and 1167.05 mg GAE.100g-1, respectively). The accessions CA and TC had the highest concentration of the major compound rutin (436.78 and 442.94 mg.100g-1, respectively). Antioxidant activity values ranged from 125.95 to 158.67 g.g-1 DPPH. Principal component analysis grouped the accessions into four groups, due to the genetic variability verified in previous studies. The results will be useful to guide actions of selection and future breeding program of the species. RESUMO A mangabeira (Hancornia speciosa Gomes) é uma espécie frutífera nativa do Brasil, pertencente à família Apocynaceae. Seu cultivo é predominantemente extrativista e suas propriedades antioxidantes têm sido recentemente relatadas. O objetivo do presente trabalho foi avaliar os compostos bioativos e atividade antioxidante em frutos de acessos oriundos do Banco Ativo de Germoplasma da Embrapa Tabuleiros Costeiros (BI, CA, LG, PR, PT e TC). Os extratos foram analisados em cromatografía líquida de alta eficiência (HPLC) e identificou-se o ácido clorogênico (93,71 - 131,66 mg.100g-1); ácido ferúlico (0,85 - 2,27 mg.100g-1) e rutina (238,59 - 442,94 mg.100g-1). Os acessos CA e PR apresentaram os maiores valores de fenóis totais (1179,39 e 1167,05 mg EAG.100g-1, respectivamente). Os acessos CA e TC apresentaram a maior concentração do composto majoritário rutina (436,78 e 442,94 mg.100g-1, respectivamente). Os valores da atividade antioxidante variaram entre 125,95 e 158,67 g.g-1 DPPH. A análise de componentes principais agrupou os acessos em quatro grupos, em consequência da variabilidade genética previamente verificada em estudos anteriores. Os resultados serão úteis para direcionar ações de seleção e futuro programa de melhoramento genético da espécie.

The ex situ conservation of seeds in cryobanks demands previous studies of dehydration procedures for the adjustment of the adequate moisture that makes long-term conservation possible. The objective of this work was to evaluate the effect of dehydration time on the regenerative capacity of embryonic axes of two genipap accessions (Umbaúba and Núcleo Bandeirante). Seeds were submitted to desiccation in policarbonate box containing silica gel for 0, 12, 16 and 20 h at room temperature and after each period, seed moisture content was determined. Seeds were inoculated in germination medium and samples were inserted into cryotubes and immersed in liquid nitrogen (-196ºC). Desiccation in silica gel was efficient in reducing the moisture content in both accessions. Umbaúba accession presented 100% germination at all desiccation treatments before cryopreservation and Núcleo Bandeirante was more sensitive to desiccation with 10% germination in 20 h. Umbaúba accession showed a reduction of growth variables with increased desiccation time. The treatments tested did not affect the growth variables of Núcleo Bandeirante accession. The desiccation, although changes observed in the ultra-structures of embryonic axis, did not influence the regeneration percentage of cryopreserved accessions.

The aim of this study was to evaluate the in vitro morphogenic potential of genipap (Genipa americana L.) zygotic embryos. Seeds obtained from ripe fruits had their zygotic embryos excised and inoculated in MS medium with 4.44µM of 6-benzylaminopurine (BAP) and supplemented with 0.0; 1.07; 2.14 and 3.21µM of naphthalene acetic acid (NAA). The potential of explants regeneration and the shoot length and number of leaves in plantlets were evaluated. The in vitro regeneration of genipap is possible from the conversion of zygotic embryos in a MS medium with 4.44µM BAP supplemented with 3.21µM NAA. RESUMO: O objetivo do trabalho foi avaliar o potencial morfogênico in vitro de embriões zigóticos de jenipapeiro (Genipa americana L.). Sementes obtidas de frutos tiveram seus embriões zigóticos excisados e inoculados em meio MS com 4,44µM de 6-benzilaminopurina (BAP) suplementado com 0,0; 1,07; 2,14 e 3,21µM de ácido naftaleno acético (ANA). O potencial de regeneração dos explantes e o comprimento da parte aérea e o número de folhas nas plântulas formadas foi avaliado. Observou-se que é possível a regeneração in vitro de jenipapeiro a partir da conversão de embriões zigóticos em meio MS com 4,44µM de BAP, suplementado com 3,21µM de ANA.

Hancornia speciosahas bioactive molecules such as phenolic compounds which exhibit antioxidant and anti-microbial properties. Nevertheless, the separation of these substances from a medicinal plant can be differentaccording to the extraction process and solvent used. Thus, the present study aimed was to separate phenoliccompounds fromHancornia speciosaleaves in three steps by using a sequential pressurized liquid extraction andevaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to60° C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 ± 0.2%)showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 °C exhibited thehighest amount of total phenolic compounds (347.0 ± 4.12 mg/g extract). Fractionated ethyl acetate extract(FAE) at 60 °C presented improved quantity of totalflavonoids (230.4 ± 3.5 mg/g extract) and rutin content(16.6 ± 0.3 mg/mL extract). The antioxidant activity results for IC50of DPPH indicated that FEE(3.8 ± 0.1 ?g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of mul-tidrug-resistantE. coli. Made available in DSpace on 2020-01-14T18:12:36Z (GMT). No. of bitstreams: 1 1s2.0S138358661930334Xmain.pdf: 429605 bytes, checksum: 20ef950a051821a5194a4f8d0e0b85df (MD5) Previous issue date: 2019

Made available in DSpace on 2020-01-14T18:11:40Z (GMT). No. of bitstreams: 1 5631172761PB.pdf: 1838315 bytes, checksum: d984c62afca3a68e7cf8670714980a6f (MD5) Previous issue date: 2019

Made available in DSpace on 2019-03-30T00:31:45Z (GMT). No. of bitstreams: 1 geneticdiversitymangabagenebankusing.pdf: 287372 bytes, checksum: edc2dc58ce0564fe492c8d3b83b3d3f8 (MD5) Previous issue date: 2019

Made available in DSpace on 2019-03-29T00:33:58Z (GMT). No. of bitstreams: 1 geneticdiversitynativegenipaamericana.pdf: 276785 bytes, checksum: 2084478b49abdc372dee0fb5db61b72e (MD5) Previous issue date: 2019

Made available in DSpace on 2020-01-14T18:12:13Z (GMT). No. of bitstreams: 1 5462217302PB.pdf: 784123 bytes, checksum: c1b665f2bd9974659c85e6e7800239f8 (MD5) Previous issue date: 2019

Made available in DSpace on 2019-12-05T18:13:55Z (GMT). No. of bitstreams: 1 CTBEB.MS.ID.555995.pdf: 624321 bytes, checksum: c577cd20d1d85ed4aca041e6c9a3abc9 (MD5) Previous issue date: 2019

Made available in DSpace on 2019-02-01T23:35:22Z (GMT). No. of bitstreams: 1 Antioxidant.pdf: 396519 bytes, checksum: acfb7372790fcc80a6a8e32844b15c10 (MD5) Previous issue date: 2018-12-28

Made available in DSpace on 2019-01-31T23:42:56Z (GMT). No. of bitstreams: 1 Geneticdiversity.pdf: 717626 bytes, checksum: 0f2c116c17763b66d5a65d8e97d6fb1a (MD5) Previous issue date: 2018-12-28

Made available in DSpace on 2019-03-30T00:31:59Z (GMT). No. of bitstreams: 1 Evaluation.pdf: 225433 bytes, checksum: 692ffb72ed8d20552e4298aacaea866d (MD5) Previous issue date: 2018

Made available in DSpace on 2018-12-29T23:30:28Z (GMT). No. of bitstreams: 1 Inductionand.pdf: 129123 bytes, checksum: 09f4f2e24a0454abbf70e502be2a5ea3 (MD5) Previous issue date: 2018-12-28

Made available in DSpace on 2019-02-01T23:35:35Z (GMT). No. of bitstreams: 1 Effectof.pdf: 1519184 bytes, checksum: 97b1297f4609bef301b730dc931341b8 (MD5) Previous issue date: 2018-12-28

Made available in DSpace on 2018-01-31T00:49:58Z (GMT). No. of bitstreams: 1 EXTRACAODEDNAEMMANGABEIRAHancorniaspeciosaGomes.pdf: 417996 bytes, checksum: f19db142afd8d17177f468ee961bcc5d (MD5) Previous issue date: 2018-01-30

Made available in DSpace on 2017-09-29T10:33:50Z (GMT). No. of bitstreams: 1 Artigo6MangabaCAjun.pdf: 774987 bytes, checksum: e3d0c5913c0a9898c88aba6e602861ec (MD5) Previous issue date: 2017-09-28

Made available in DSpace on 2018-01-26T23:43:27Z (GMT). No. of bitstreams: 1 AV1.pdf: 451417 bytes, checksum: 8912979d21acb1d6813cb88c90b1f2d5 (MD5) Previous issue date: 2017-01-20

Cambui (Myrciaria tenella O. Berg) is a native species from Brazil, which belongs to the family Myrtaceae. Molecular characterization is one of the most used tools for the study of the biotechnological potential of species because the diversity level between individuals can be inferred. Analysis of enetic diversity is fundamental to the direction of the strategies necessary to form and maintain a germplasm. This study aimed to evaluate the genetic diversity in a natural population of cambui using inter-simple sequence repeat (ISSR) molecular markers. The natural population, which provided the plant material, is found at the Private Reserve of Natural Heritage of Caju, which belongs to the experimental field of Embrapa Tabuleiros Costeiros, in the municipality of Itaporanga d’Ajuda, SE, Brazil. Young leaves of each individual were collected for DNA extraction and analysis of PCR-ISSR. Thirty primers were tested and the top 10 were selected. The use of these primers resulted in 71 fragments with 98.3% polymorphism. Similarity of individuals ranged between 0.30 and 0.92. The most similar individuals were C13 and C17 and the most distant were C1 and C41. Through UPGMA, six distinct groups were identified. This information may be used for conservation of these genetic resources, germplasm exchange, creation of germplasm bank and in future studies with this species. Made available in DSpace on 2018-01-26T23:43:50Z (GMT). No. of bitstreams: 1 AV3.pdf: 1169452 bytes, checksum: c364a3570c8658819048eacf2ab64212 (MD5) Previous issue date: 2017-01-20

O objetivo do presente trabalho foi estudar as respostas morfogenéticas de explantes de jenipapeiro em diferentes condições de cultura in vitro para subsidiar futuros protocolos de multiplicação e produção in vitro de metabólitos secundários. Após 90 dias de cultivo, as plântulas de jenipapeiro foram segmentadas (segmentos nodal e foliar) e transferidas para meio MS suplementado com 30 g L-1 de sacarose e diferentes concentrações de 2,4 - Diclorofenoxiacético - 2,4-D (0; 4 e 8 mg L-1) combinadas com quatro concentrações de benzilaminopurina-BAP (0; 1; 2 e 3 mg L-1). Aos 120 dias a porcentagem de explantes com resposta morfoge-nética quanto à formação de calos e/ou organogênese foi avaliada. O 2,4-D nas concentrações de 4 e 8 mg L-1 induz aumento na resposta morfogenética, principalmente na formação de calos, segmentos foliar e nodal de Genipa americana L. acesso SIR. O BAP a 1,77 mg L-1 induz maior formação de calos em segmentos foliares e maior regeneração de brotos nos segmentos foliar e nodal. As concentrações de 2,4-D e BAP estudadas não induzem à formação de calos embriogênicos nos segmentos foliar e nodal de jenipapeiro do acesso SIR

Made available in DSpace on 2017-07-19T23:53:53Z (GMT). No. of bitstreams: 1 Physiological.pdf: 330863 bytes, checksum: ea4c5112dd905d0b0528ddee7f43ab8a (MD5) Previous issue date: 2016-04-07

Made available in DSpace on 2017-07-19T23:53:25Z (GMT). No. of bitstreams: 1 Josue.artigoAfricanGenipa.pdf: 452953 bytes, checksum: 0173b8639fcaf5367e5ae94ea407a28d (MD5) Previous issue date: 2016-01-26

Made available in DSpace on 2018-01-26T23:42:36Z (GMT). No. of bitstreams: 1 diversityandgenetic.pdf: 774691 bytes, checksum: eb7b21b3d34f111e2d5febcace4cba74 (MD5) Previous issue date: 2016-04-07