Search

Your search keyword '"Reiter, Eric"' showing total 27 results
Start Over Author "Reiter, Eric" Topic fsh
27 results on '"Reiter, Eric"'

1. Receptors | Thyroid-Stimulating Hormone/Luteinizing Hormone/Follicle-Stimulating Hormone Receptors

Author

Combarnous, Yves, Crépieux, Pascale, Reiter, Eric, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Dynamiques de populations multi-échelles pour des systèmes physiologiques (MUSCA), Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE)

Subjects
LH, Gonadotropin, Thyroid, endocrine system, 0303 health sciences, TSH, [SDV]Life Sciences [q-bio], Lutropin, Ovary, Thyrotropin, Follitropin, TSHR, 03 medical and health sciences, Thyrotropin receptor autoantibodies TRAbs, 0302 clinical medicine, FSHR, 030220 oncology & carcinogenesis, FSH, LHCGR, Glycoprotein hormone, G protein-coupled receptor, Testes, hormones, hormone substitutes, and hormone antagonists, 030304 developmental biology
Abstract

International audience; The thyroid-stimulating hormone receptor (TSHR), luteinizing hormone receptor (LHCGR), and follicle-stimulating hormone receptor (FSHR) are collectively referred to as the glycoprotein hormone receptors because they bind structurally similar glycoprotein hormones. The glycoprotein hormones consist of the pituitary thyroid-stimulating hormone (thyrotropin, TSH), luteinizing hormone (lutropin, LH), and follicle-stimulating hormone (follitropin, FSH) as well as the chorionic gonadotropin (choriogonadotropin, CG) placental hormone. The glycoprotein hormones are each composed of two dissimilar subunits (α and β) that are noncovalently associated. Within a given species, the α-subunit is identical and the β-subunits are distinct but homologous. Due to the largely similar nature of LHβ and CGβ, the LHCGR binds either LH or CG. The binding specificity of LH/CG to the LHCGR, FSH to the FSHR, and TSH to the TSHR is fairly strict. However, in some cases, extremely high levels of hormone can cause activation of the inappropriate receptor. Because the LHCGR and FSHR are localized primarily to the gonads, these two receptors are also referred to as the gonadotropin receptors. The glycoprotein hormone receptors belong to the family A (rhodopsin-like) of G protein-coupled receptors (GPCRs). However, they share the relatively unique feature of having a large extracellular domain that mediates high affinity binding of the hormone. In spite of their different physiological roles, the glycoprotein hormone receptors share a similar structural organization and mechanism of action.

Published
2021
Full Text
View/download PDF

3. Pleotropism of gonadotropin action

Author

Simoni, Manuela, Paradiso, Elia, Lockhart, Véronique, Reiter, Eric, Casarini, Livio, Pellissier, Lucie, Crépieux, Pascale, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Università degli Studi di Modena e Reggio Emilia = University of Modena and Reggio Emilia (UNIMORE), LE STUDIUM (LE STUDIUM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de recherche pour le développement [IRD] : UR-Centre National de la Recherche Scientifique (CNRS), This project has been supported by LE STUDIUM Loire Valley Institute for Advanced Studies, Orléans & Tours, France with funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 665790, Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Università degli Studi di Modena e Reggio Emilia (UNIMORE), Bureau de Recherches Géologiques et Minières (BRGM) (BRGM)-Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de recherche pour le développement [IRD] : UR-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Gonadotropin action, LH, endocrine system, Granulosa cells, FSHR, FOS: Biological sciences, [SDV]Life Sciences [q-bio], FSH, FOS: Health sciences, infertility, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

LE STUDIUM Multidisciplinary Journal. This is the final version of the article, which has been published in final form at http://www.lestudium-ias.com/content/pleotropism-gonadotropin-action-0; International audience; Evidence exists that the gonadotropins LH and FSH can substitute to each other under certain circumstances, in addition to the fact that they can act together in granulosa cells. The aim of this study is to investigate how the two human gonadotropins influence each other in granulosa cells expressing both receptors, or by co-culturing cells expressing either the LHCGR or the FSHR (as a model granulosa/theca interaction). Plasmids encoding the c-myc-tagged-LHCGR and the FLAG-tagged FSHR under the control of an inducible coumermycin-responsive or doxycycline-responsive promoter, respectively were produced. These plasmids were used to permanently transfect human granulosa cell-derived KGN cells and HEK293 cells. The following cell lines were obtained and partially characterized: #1 c-myc-tagged-LHCGR-KGN; #2 FLAG-tagged FSHR_HEK293; #3 FLAG-tagged FSHR-KGN; #4 Double, c-myc-tagged-LHCGR and FLAG-tagged FSHR-KGN. After induction of receptor expression, the cell lines #1 and #2 and #3 responded to hCG and FSH stimulation, respectively by producing cAMP. Receptor expression was demonstrated by RT-PCR and flow cytometry. The characterization of the cell line #4 is ongoing. These cell lines are now available for the study of cell signaling and steroid synthesis, as well as in silico modeling, to gain insight into the dynamics of the intertwined cell response to FSH and LH in granulosa cells. These experiments will continue in parallel in both laboratories involved. Our cell lines represent new, very valuable instruments for the study of molecular pharmacology of FSH and LH, in order to improve infertility treatment, (multi)follicular growth for assisted reproduction, ovulation and spermatogenesis.

Published
2020
Full Text
View/download PDF

4. The translatome of the FSH receptor reveals tha several FSH-responsive signaling effectors are co-translated in primary rat Sertoli cells

Author

Trefier, Aurélie, Yvinec, Romain, Bourquard, Thomas, Léon-Huaman, Kelly, Musnier, Astrid, Boulo, Thomas, Morales, Julia, Langonné, Nathalie, Guillou, Florian, Reiter, Eric, Poupon, Anne, Crepieux, Pascale, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), UMR 8227, Centre National de la Recherche Scientifique (CNRS), Sorbonne Universités, Université Pierre et Marie Curie (Paris 6), INRA PHASE Department, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 (UPMC), Université François Rabelais (Tours). Tours, FRA. Centre National de la Recherche Scientifique (CNRS), FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Archive Ouverte, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Station biologique de Roscoff [Roscoff] (SBR), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
endocrine system, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], fsh receptor, [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], récepteur hormonal, translatome, G protein coupled receptor, récepteur couplé aux protéines G, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, récepteur fsh, GPCR, signalisation, FSH, cellule de sertoli, hormonal receptor, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, hormones, hormone substitutes, and hormone antagonists
Abstract

The translatome of the FSH receptor reveals tha several FSH-responsive signaling effectors are co-translated in primary rat Sertoli cells. 2. Journées du GdR 3606 Repro

Published
2017

5. Intégration des contraintes spatiales dans la modélisation dynamique d’un système biologique complexe : le réseau de signalisation FSH

Author

Ayoub, Mohammed Akli, Boulo, Thomas, Bourquard, Thomas, Crépieux, Pascale, Guillou, Florian Jean Louis, León, Kelly, Morales, Julia, Musnier, Astrid, Poupon, Anne, Reiter, Eric, Tréfier, Aurélie, Yvinec, Romain, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de Recherche Agronomique (INRA). UAR Département Physiologie Animale et Systèmes d'Elevage (0558)., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], gpcr, cellule de Sertoli, [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], fsh, traduction régionalisé, ComputingMilieux_MISCELLANEOUS, modélisation
Abstract

National audience

Published
2016

6. Glycosylation Pattern and in vitro Bioactivity of Reference Follitropin alfa and Biosimilars.

Author

Riccetti, Laura, Sperduti, Samantha, Lazzaretti, Clara, Klett, Danièle, De Pascali, Francesco, Paradiso, Elia, Limoncella, Silvia, Potì, Francesco, Tagliavini, Simonetta, Trenti, Tommaso, Galano, Eugenio, Palmese, Angelo, Satwekar, Abhijeet, Daolio, Jessica, Nicoli, Alessia, Villani, Maria Teresa, Aguzzoli, Lorenzo, Reiter, Eric, Simoni, Manuela, and Casarini, Livio

Subjects
GONADOTROPIN, GLYCANS, FOLLICLE-stimulating hormone, BIOSIMILARS, GLYCOSYLATION, KRUSKAL-Wallis Test, GRANULOSA cells
Abstract

Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use. They have specific FSH activity and a unique glycosylation profile dependent on source cells. The aim of the study is to compare the originator (reference) follitropin alfa (Gonal-f®)- with biosimilar preparations (Bemfola® and Ovaleap®)-induced cellular responses in vitro. Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns of glycan species (Kruskal-Wallis test; p < 0.05, n = 6) and by glycotope mapping. Increasing concentrations of Gonal-f® or biosimilar (1 × 10−3-1 × 103 ng/ml) were used for treating human primary granulosa lutein cells (hGLC) and FSH receptor (FSHR)-transfected HEK293 cells in vitro. Intracellular cAMP production, Ca2+ increase and β-arrestin 2 recruitment were evaluated by BRET, CREB, and ERK1/2 phosphorylation by Western blotting. 12-h gene expression, and 8- and 24-h progesterone and estradiol synthesis were measured by real-time PCR and immunoassay, respectively. We found preparation-specific glycosylation patterns by lectin assay (Kruskal-Wallis test; p < 0.001; n = 6), and similar cAMP production and β-arrestin 2 recruitment in FSHR-transfected HEK293 cells (cAMP EC50 range = 12 ± 0.9–24 ± 1.7 ng/ml; β-arrestin 2 EC50 range = 140 ± 14.1–313 ± 18.7 ng/ml; Kruskal-Wallis test; p ≥ 0.05; n = 4). Kinetics analysis revealed that intracellular Ca2+ increased upon cell treatment by 4 μg/ml Gonal-f®, while equal concentrations of biosimilars failed to induced a response (Kruskal-Wallis test; p < 0.05; n = 3). All preparations induced both 8 and 24 h-progesterone and estradiol synthesis in hGLC, while no different EC50s were demonstrated (Kruskal-Wallis test; p > 0.05; n = 5). Apart from preparation-specific intracellular Ca2+ increases achieved at supra-physiological hormone doses, all compounds induced similar intracellular responses and steroidogenesis, reflecting similar bioactivity, and overall structural homogeneity. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

7. Dynamic model of FSH signalling pathways in Sertoli cells adapted to two stages

Author

Heitzler, Domitille, Musnier, Astrid, Boulo, Thomas, Tesseraud, Sophie, Durand, Guillaume, Lécureuil, C., Guillou, H., Reiter, Eric, Crepieux, Pascale, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Constraint programming (CONTRAINTES), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), The Babraham Institute [Cambridge, UK], ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Babraham Institute, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], SIGNAL INTRACELLULAIRE, ODE, [SDV]Life Sciences [q-bio], FSH, RAT, [INFO]Computer Science [cs], [INFO] Computer Science [cs], rpS6, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2009

8. Developmentally regulated integration of PKA- and PI-3 kinase-mediated signalling on p70S6K by the FSH-R

Author

Musnier, Astrid, Lécureuil, C., Tesseraud, Sophie, Boulo, Thomas, Durand, Guillaume, Guillou, H., Reiter, Eric, Crepieux, Pascale, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), The Inositide Laboratory, Babraham Institute, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], FSH, HORMONE GONADOTROPE, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2008

9. Selective ß-arrestin-dependent signalling by FSHR mutants

Author

Durand, Guillaume, Piketty, Vincent, Heitzler, Domitille, Tranchant, Thibaud, Musnier, Astrid, Wehbi, Vanessa, Decourtye, Jeremy, Gauthier, Christophe, Dupuy, Laurence, Maurel, Marie-Christine, Yao, Richard, Lichtarge, Oiivier, Crépieux, Pascale, Reiter, Eric, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Department of Molecular and Human Genetics, Baylor College of Medicine (BCM), Baylor University-Baylor University, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], FSH, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2008

10. Modelling of FSH receptor signalling network

Author

Heitzler, Domitille, Fages, F., Clement, Frédéric, Reiter, Eric, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Constraint programming (CONTRAINTES), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), SIgnals and SYstems in PHysiology & Engineering (SISYPHE), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], FSH, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, RECEPTEUR
Abstract

International audience

Published
2008

11. Modelling of FSHR-induced signalling network

Author

Heitzler, Domitille, Durand, Guillaume, Dupuy, Laurence, Gauthier, Christophe, Piketty, Vincent, Crepieux, Pascale, Rizk, Aurélien, Soliman, Sylvain, Fages, Francois, Clement, Frédéric, Reiter, Eric, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Constraint programming (CONTRAINTES), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), SIgnals and SYstems in PHysiology & Engineering (SISYPHE), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], RESEAU DE SIGNALISATION, [SDV]Life Sciences [q-bio], FSH, [INFO]Computer Science [cs], BETA-ARRESTINE, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, MECANISME DE TRANSDUCTION
Abstract

International audience

Published
2008

12. Modelling of FSH signalling network in ovarian follicle cells with BIOCHAM

Author

Clement, F., Fages, F., Heitzler, Domitille, Reiter, Eric, SIgnals and SYstems in PHysiology & Engineering (SISYPHE), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Constraint programming (CONTRAINTES), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], FSH, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2007

13. Bases moléculaires de la modulation de la réponse FSH et LH de la eCG induite par des anticorps anti-eCG potentialisants

Author

Wehbi, Vanessa, Decourtye, Jeremy, Durand, Guillaume, Reiter, Eric, Maurel, Marie-Christine, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
LH, [SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], eCG, FSH, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, VOIE DE SIGNALISATION
Abstract

National audience

Published
2007

14. G protein-coupled receptor kinase 2 and beta-arrestins are recruited to FSH receptor in stimulated rat primary Sertoli cells

Author

Marion, Sébastien, Kara, Elodie, Crépieux, Pascale, Piketty, Vincent, Martinat, Nadine, Guillou, Florian Jean Louis, Reiter, Eric, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, FSH, RAT, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

15. Multiple roles for beta-arrestins in FSH signaling: a 5 Ser/Thr cluster in the C-terminus of the receptor is important for beta-arrestin recruitment, desensitization and internalization but not beta-arrestin-mediated ERK activation

Author

Kara, Elodie, Crépieux, Pascale, Gauthier, Christophe, Martinat, Nadine, Piketty, Vincent, Guillou, Florian Jean Louis, Reiter, Eric, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], ERK, [SDV]Life Sciences [q-bio], FSH, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, RECEPTEUR
Abstract

National audience

Published
2005

16. Role of a serine/threonine cluster present in the C-terminal tail of the follicle stimulating hormone receptor

Author

Kara, Elodie, Gauthier, Christophe, Martinat, Nadine, Marion, S., Piketty, Vincent, Guillou, Florian Jean Louis, Crepieux, Pascale, Reiter, Eric, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], GRK, [SDV]Life Sciences [q-bio], FSH, [INFO]Computer Science [cs], HORMONE GONADOTROPE, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2005

17. FSH activates p70s6 kinase by protein kinase A-mediated dephosphorylation of Thr421/Ser424 and modulates mRNA translability in differentiated primary Sertoli cells

Author

Lécureuil, C., Tesseraud, Sophie, Kara, Elodie, Martinat, Nadine, Sow, A., Fontaine, I., Gauthier, Christophe, Reiter, Eric, Guillou, Florian Jean Louis, Crepieux, Pascale, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], FSH, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2004

18. G Protein-coupled receptor kinases and beta arrestins are relocalized and attenuate cyclic 3', 5' -adenosine monophosphate response to follicle-stimulating hormone in rat primary sertoli cells

Author

Marion, S., Robert, F., Crépieux, Pascale, Martinat, Nadine, Troispoux, Carine, Guillou, Florian Jean Louis, Reiter, Eric, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
FSH, AMP-C, RAT, HORMONE GONADOTROPE, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2002

19. A novel messenger ribonucleic acid homologous to human MAGE-D is strongly expressed in rat Sertoli cells and weakly in Leydig cells and is regulated by follitropin, lutropin, and prolactin

Author

Hennuy, B., Reiter, Eric, Cornet, Aziyadé, Bruyninx, M., Daukandt, M., Houssa, P., N'Guyen, V.H., CLOSSET, J., HENNEN, G., ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], EXPRESSION DU GENE, [SDV]Life Sciences [q-bio], FSH, RAT, [INFO]Computer Science [cs], HORMONE GONADOTROPE, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2000

20. Beta 2 adrenergic receptors mediate cAMP, tissue-type plasminogen activator and transferrin production in rat Sertoli cells

Author

Troispoux, Carine, Reiter, Eric, Combarnous, Yves, Guillou, Florian Jean Louis, Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], FSH, AMP-C, RAT, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, SERTOLI
Abstract

International audience

Published
1998

21. Direct impact of gonadotropins on glucose uptake and storage in preovulatory granulosa cells: Implications in the pathogenesis of polycystic ovary syndrome.

Author

Chahal, Nidhi, Geethadevi, Anjali, Kaur, Surleen, Lakra, Ruchi, Nagendra, Anjali, Shrivastav, T.G., De Pascali, Francesco, Reiter, Eric, Crépieux, Pascale, Devi, M. Gouri, Malhotra, Neena, Muralidhar, K., and Singh, Rita

Subjects
GRANULOSA cells, POLYCYSTIC ovary syndrome, GLUCOSE, PHOSPHOPROTEIN phosphatases, GLYCOGEN
Abstract

Polycystic ovary syndrome (PCOS) is often associated with higher levels of LH, and arrested ovarian follicular growth. The direct impact of high LH on FSH mediated metabolic responses in PCOS patients is not clearly understood. In order to investigate the impact of FSH and LH on glucose metabolism in preovulatory granulosa cells (GCs), we used [U 14C]-2 deoxyglucose, D-[U 14C]-glucose or 2-NBD glucose to analyse glucose uptake and its incorporation into glycogen. To reproduce the high androgenic potential in PCOS patients, we administered hCG both in vitro and in vivo. The role of IRS-2/PI3K/Akt2 pathway was studied after knockdown with specific siRNA. Immunoprecipitation and specific assays were used for the assessment of IRS-2, glycogen synthase and protein phosphatase 1. Furthermore, we examined the in vivo effects of hCG on FSH mediated glycogen increase in normal and PCOS rat model. HEK293 cells co-expressing FSHR and LHR were used to demonstrate glucose uptake and BRET change by FSH and hCG. In normal human and rat granulosa cells, FSH is more potent than hCG in stimulating glucose uptake, however glycogen synthesis was significantly upregulated only by FSH through increase in activity of glycogen synthase via IRS-2/PI3K/Akt2 pathway. On the contrary, an impaired FSH-stimulated glucose uptake and glycogen synthesis in granulosa cells of PCOS-patients indicated a selective defect in FSHR activation. Further, in normal human granulosa cells, and in immature rat model, the impact of hCG on FSH responses was such that it inhibited the FSH-mediated glucose uptake as well as glycogen synthesis through inhibition of FSH-stimulated IRS-2 expression. These findings were further validated in HEK293 cells overexpressing Flag-LHR and HA-FSHR, where high hCG inhibited the FSH-stimulated glucose uptake. Notably, an increased BRET change was observed in HEK293 cells expressing FSHR-Rluc8 and LHR-Venus possibly suggesting increased heteromerization of LHR and FSHR in the presence of both hCG and FSH in comparison to FSH or hCG alone. Our findings confirm a selective attenuation of metabolic responses to FSH such as glucose uptake and glycogen synthesis by high activation level of LHR leading to the inhibition of IRS-2 pathway, resulting in depleted glycogen stores and follicular growth arrest in PCOS patients. • Gonadotropins have a direct role in glucose uptake, storage and utilization in preovulatory granulosa cells. • FSH and not hCG upregulates glycogen synthesis through IRS-2 pathway. • PCOS patients with or without insulin resistance have impaired FSH-stimulated glucose uptake and glycogen synthesis. • Attenuation of FSH-stimulated glucose uptake and storage results after co-treatments with FSH and hCG. • Defective glucose uptake in HEK293 cells in the presence of FSH+hCG is associated with increased proximity of FSHR and LHR. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

22. Follicle-Stimulating Hormone (FSH) Action on Spermatogenesis: A Focus on Physiological and Therapeutic Roles.

Author

Santi, Daniele, Crépieux, Pascale, Reiter, Eric, Spaggiari, Giorgia, Brigante, Giulia, Casarini, Livio, Rochira, Vincenzo, and Simoni, Manuela

Subjects
FOLLICLE-stimulating hormone, SPERMATOGENESIS, HUMAN reproduction, SERTOLI cells, MALE infertility
Abstract

Background: Human reproduction is regulated by the combined action of the follicle-stimulating hormone (FSH) and the luteinizing hormone (LH) on the gonads. Although FSH is largely used in female reproduction, in particular in women attending assisted reproductive techniques to stimulate multi-follicular growth, its efficacy in men with idiopathic infertility is not clearly demonstrated. Indeed, whether FSH administration improves fertility in patients with hypogonadotropic hypogonadism, the therapeutic benefit in men presenting alterations in sperm production despite normal FSH serum levels is still unclear. In the present review, we evaluate the potential pharmacological benefits of FSH administration in clinical practice. Methods: This is a narrative review, describing the FSH physiological role in spermatogenesis and its potential therapeutic action in men. Results: The FSH role on male fertility is reviewed starting from the physiological control of spermatogenesis, throughout its mechanism of action in Sertoli cells, the genetic regulation of its action on spermatogenesis, until the therapeutic options available to improve sperm production. Conclusion: FSH administration in infertile men has potential benefits, although its action should be considered by evaluating its synergic action with testosterone, and well-controlled, powerful trials are required. Prospective studies and new compounds could be developed in the near future. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

23. FSH for the Treatment of Male Infertility.

Author

Casarini, Livio, Crépieux, Pascale, Reiter, Eric, Lazzaretti, Clara, Paradiso, Elia, Rochira, Vincenzo, Brigante, Giulia, Santi, Daniele, and Simoni, Manuela

Subjects
LUTEINIZING hormone releasing hormone, SERTOLI cells, MALE sterility in plants, OLIGOSPERMIA, MALE infertility, FOLLICLE-stimulating hormone, TARGETED drug delivery, INFERTILITY
Abstract

Follicle-stimulating hormone (FSH) supports spermatogenesis acting via its receptor (FSHR), which activates trophic effects in gonadal Sertoli cells. These pathways are targeted by hormonal drugs used for clinical treatment of infertile men, mainly belonging to sub-groups defined as hypogonadotropic hypogonadism or idiopathic infertility. While, in the first case, fertility may be efficiently restored by specific treatments, such as pulsatile gonadotropin releasing hormone (GnRH) or choriogonadotropin (hCG) alone or in combination with FSH, less is known about the efficacy of FSH in supporting the treatment of male idiopathic infertility. This review focuses on the role of FSH in the clinical approach to male reproduction, addressing the state-of-the-art from the little data available and discussing the pharmacological evidence. New compounds, such as allosteric ligands, dually active, chimeric gonadotropins and immunoglobulins, may represent interesting avenues for future personalized, pharmacological approaches to male infertility. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

24. Biased signalling in follicle stimulating hormone action.

Author

Landomiel, Flavie, Gallay, Nathalie, Jégot, Gwenhael, Tranchant, Thibaud, Durand, Guillaume, Bourquard, Thomas, Crépieux, Pascale, Poupon, Anne, and Reiter, Eric

Subjects
*FOLLICLE-stimulating hormone, *CELLULAR signal transduction, *G protein coupled receptors, *PROTEIN conformation, *CYCLIC adenylic acid, *GENETIC polymorphisms
Abstract

Highlights: [•] FSHR-induced signalling is a highly complex process that is not limited to coupling with the Gαs–cAMP–PKA pathway. [•] A specific subset of GPCR conformations are stabilized upon ligand binding and association with effector proteins. [•] It is possible to selectively control the signalling pathways activated at the FSHR using biased ligands. [•] Specific mutations or polymorphisms occurring at the receptor level can lead to biased signalling. [•] It is crucial to use adapted experimental methods and data processing to properly detect and quantify signalling biases. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
View/download PDF

25. Preferential β-arrestin signalling at low receptor density revealed by functional characterization of the human FSH receptor A189 V mutation

Author

Tranchant, Thibaud, Durand, Guillaume, Gauthier, Christophe, Crépieux, Pascale, Ulloa-Aguirre, Alfredo, Royère, Dominique, and Reiter, Eric

Subjects
*GENETIC mutation, *CELLULAR signal transduction, *PHOSPHORYLATION, *CELL membranes, *GENE expression, *HUMAN fertility, *PROTEIN kinases
Abstract

Abstract: The A189 V inactivating mutation of the human FSH receptor (FSHR) leads to subfertility in men and primary ovarian failure in women. This mutation has previously been associated with intracellular retention of the FSHR and impaired cAMP production. Here, we show that the A189 V FSHR stably expressed in HEK293N cells provoked ERK MAP kinases phosphorylation through β-arrestins, independently of the canonical cAMP/PKA pathway. Interesting, both the A189 V and wild-type (Wt) FSHRs selectively activated cAMP-independent ERK phosphorylation when expressed at low plasma membrane densities. These data indicate that the selective intracellular signalling triggered by the A189 V FSHR resulted from reduced membrane expression rather than by switching receptor coupling. Hence, receptor density at the plasma membrane might control the balance between distinct signal transduction mechanisms. Furthermore, our results help to clarify why mutations of FSHβ are more deleterious to human fertility than the FSHR A189 V mutation which preserves parts of receptor signalling repertoire. [ABSTRACT FROM AUTHOR]

Published
2011
Full Text
View/download PDF

26. FSH-stimulated PTEN activity accounts for the lack of FSH mitogenic effect in prepubertal rat Sertoli cells

Author

Dupont, Joëlle, Musnier, Astrid, Decourtye, Jérémy, Boulo, Thomas, Lécureuil, Charlotte, Guillou, Hervé, Valet, Sophie, Fouchécourt, Sophie, Pitetti, Jean-Luc, Nef, Serge, Reiter, Eric, and Crépieux, Pascale

Subjects
*FOLLICLE-stimulating hormone, *SERTOLI cells, *LABORATORY rats, *LEYDIG cells, *PHOSPHOINOSITIDES, *PHOSPHATASES, *CHROMOSOMES
Abstract

Abstract: Follicle-stimulating hormone (FSH) controls the proliferation and differentiation of Sertoli cells of the testis. FSH binds a G protein-coupled receptor (GPCR) to stimulate downstream effectors of the phosphoinositide-3 kinase (PI3K)-dependent pathway, without enhancing PI3K activity. To clarify this paradox, we explored the activity of phosphatase and tensin homolog deleted in chromosome 10 (PTEN), the PI3K major regulator, in primary cultures of rat Sertoli cells. We show that, within minutes, FSH increases PTEN neo-synthesis, requiring the proteasomal degradation of an unidentified intermediate, as well as PTEN enzymatic activity. Importantly, introducing an antisense cDNA of PTEN into differentiating Sertoli cells restores FSH-dependent cell proliferation. In conclusion, these results provide a new mechanism of PTEN regulation, which could serve to block entry into S phase of Sertoli cells, while they are proceeding through differentiation in prepubertal animals. [Copyright &y& Elsevier]

Published
2010
Full Text
View/download PDF

27. Dichlorodiphenyltrichloroethane impairs follicle-stimulating hormone receptor-mediated signaling in rat Sertoli cells

Author

Bernard, Laure, Martinat, Nadine, Lécureuil, Charlotte, Crépieux, Pascale, Reiter, Eric, Tilloy-Ellul, Anne, Chevalier, Stéphane, and Guillou, Florian

Subjects
*DDT (Insecticide), *ORGANOCHLORINE compounds, *SERTOLI cells, *FOLLICLE-stimulating hormone
Abstract

Abstract: Any toxicant that affects Sertoli cell development can potentially disturb male fertility. So far, the effects of organochlorine compounds have been poorly investigated in male. Here, we studied the effects of dichlorodiphenyltrichloroethane (DDT), an organochloride pesticide, on Sertoli cells. DDT inhibited the cAMP response to follicle-stimulating hormone (FSH), the major endocrine control of Sertoli cell development, and to a β2-agonist, isoproterenol. DDT exposure decreased the level of FSH binding sites. Direct adenylyl cyclase activation by Forskolin was unaltered by DDT, while the activation of Gαs by cholera toxin was decreased by DDT. The DDT inhibitory effect on the FSH response was also observed in Ser W3 cells, a Sertoli cell-derived immortalized cell line. All these effects were reproduced by the lipophilic aromatic bisphenol A but not by structurally unrelated CisPlatin. In conclusion, these results are a first step in understanding the molecular basis of DDT deleterious effects in spermatogenesis. [Copyright &y& Elsevier]

Published
2007
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results