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The article focuses on the study which identifies the causes of plant virus Nigrospora sphaerica to the leaf spots and twig and shoot blight on Blueberry in Argentina. The study evaluates pathogenicity of the leaves through the punctured of flamed needles and sprayed with spore suspension of distilled water. It was found out that after 20 days of inoculation, all wounded inoculated plants started to show disease symptoms. Moreover, fungus can create the wounds of the plants through the bites of the insects after a long-term wetness duration.