Search

Scanning electron microscopy was used to evidence the aggregated structure of a forest soil as well as the presence of fungal hyphae external to soil aggregates. The supernatant of soil suspension in water mainly contained isolated bacteria, while ultrathin sections of aggregates frequently revealed groups of bacteria surrounded by a sheath of mucilage with adhering clay minerals on the outside. These results confirm the existence of two particular biotopes in the soil studied: one is located inside aggregates, and the other, in the inter-aggregate spaces.

Variations between seasonal samplings of a forest soil were studied during a one year cycle. The total number of microorganisms was maximal in march, while qualitative variations of the bacterial and fungal flora were unimportant. Electron microscope studies showed physiological differences and enabled to evaluate the seasonal variations of the type of associations of soil microbes.