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Tea infusion is the main route of human exposure to pollutants in tea, and it is vital to investigate the transfer rate of pollutants from tea leaves to infusion. Carbendazim is a commonly used systemic fungicide, the transfer behavior of which in Yingshan Yunwu tea is not clear. Hence, this study firstly established the analytical method for determination of carbendazim with great accuracy and precision via modified QuEChERS method and ultra-performance liquid chromatography-fluorescence detection (UPLC-FLD). Then, the transfer behavior of carbendazim from tea leaves and infusion was systematically investigated. Results indicated that water temperature and proportion of tea to water ration would obviously increase the transfer rate of carbendazim, but times of infusion repetition showed negative correlation with the transfer rate of carbendazim. In addition, brewing time seemed to have lesser impact on the transfer rate. This study will be helpful for the risk assessment of carbendazim residue and provide the guidance for tea brewing. [ABSTRACT FROM AUTHOR]